Assessment of molecular markers associated with resistance or susceptibility to Fusarium wilt TR4 in Musa acuminata ssp. malaccensis / Fatimah binti Kayat
Bananaandplantain(Musaspp.)arestaplefoodcropsintropicaland subtropicalcountriesandalsoplayakeyroleintheeconomicofmanydeveloping countries.However,thetenyearreview(1996-2005)oftheproductionofthecrop showedthatthelandusageforbananaandplantainhadincreasedbuttheyieldhadbeen reducedduringthesameperiod.I...
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| Format: | Thesis |
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2009
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| Online Access: | http://pendeta.um.edu.my/client/default/search/detailnonmodal/ent:$002f$002fSD_ILS$002f796$002fSD_ILS:796891/one?qu=Assessment+of+molecular+markers+associated+with+resistance http://studentsrepo.um.edu.my/4324/1/Fatimah_Kayat_%2D_PhD_Thesis_2009.pdf |
| Summary: | Bananaandplantain(Musaspp.)arestaplefoodcropsintropicaland
subtropicalcountriesandalsoplayakeyroleintheeconomicofmanydeveloping countries.However,thetenyearreview(1996-2005)oftheproductionofthecrop showedthatthelandusageforbananaandplantainhadincreasedbuttheyieldhadbeen reducedduringthesameperiod.InMalaysia,PanamadiseaseorFusariumwiltcaused
byFusariumoxysporumf.sp.cubenseVCG01213/16alsoknownasTropicalrace4
(TR4)isthemajorandthemostdestructivediseasewhichcausesconsiderablelossto theMalaysianbananaexporttrade.Conventionalbananabreedingforresistanceto
Fusariumwiltishamperedbysterility,triploidy,longgenerationtimeandthelackof
appropriatetestingsites.Biotechnologicaladvancesinvolvinginvitropropagation,
somaticembryogenesisandmolecularmarkersprovidetoolsforgeneticrelationship
studiesandQTLanalysisthuscouldenhancethedevelopmentofcultivarsadaptedto
changingenvironments.
Theuseofgenesfromthewildcroprelativeshavebeenrecognizedinbreeding
programsofmanycrops.Inthisstudy,thestrategyapproachistodevelopnew
populationswithtraitsspecifictothepathogenfromtheindigenouswildbananaMusa
acuminatassp.malaccensis(AA)whichhasbeenshowntohaveveryhighresistanceto
FOCTR4.Maturedseedswereextractedfromfruitbunchesoffourrandomopencross
populationsofwildbananainordertoproduceseedprogeniesraisedthroughzygotic
embryoculture.Embryogerminationwasachievedwithoutanyseedtreatmentinthe
darkfortwoweeksbeforebeingplacedunderlight.Individualseedprogenieswere
subjectedtoseveralin-vitrostagesinordertogainuniformplantletswithreplicatesfor
diseasescreening.DifferentdegreesofresponsetoFusariumoxysporumf.sp.cubense
TR4wereobservedinwildbananaseedprogeniesaswellastheirF1hybridpopulations
whichprovidedapotentiallyusefulgeneticresourceforthedevelopmentofdisease
resistancemarkersthatcouldbeexploitedinamarker-assistedselection.
AhighdegreeofpolymorphisminwildbananaseedprogeniesshownbyRAPD
m
arkersprovidedgoodearlyevidenceforthepotentialusefulnessofpolymorphismandse
gregationstudiesusingmolecularmarkers.AnalysisofRAPD,STMSandAFLPm
arkersinthisstudydidnotshowanycleardifferentiationbetweenresistantandsus
ceptibleindividuals.However,markersgeneratedbythosemethodswereincludedinl
inkagemapdevelopment.Atotalof18markershadbeengeneratedfromRAPDa
nalysiswhile14SSRmarkerswereobservedfromSTMSanalysis.Atotalof4657ba
ndswasgeneratedfrom30EcoR1+3xMseI+3,oneEcoR1+3xMseI+2and43P
stI+2xMseI+3primercombinations.The639markersconsistingof607AFLPs,14S
SRsand17RAPDsmarkersweredeterminedontwomappingpopulations(resistancea
ndsusceptibilitytoFOCTR4).Atotalof471markers(286Pst-markers,155Eco-m
arkers,17RAPDmarkersand13STMSmarkers)wereanalyzedforthesusceptiblem
appingpopulationwhile414markers(249Pst-markers,143Eco-markers,9RAPDm
arkersand13STMSmarkers)wereanalyzedfortheresistantmappingpopulation.L
inkageanalysisofthedataresultedinthegenerationoftwosetsoflinkagemapsc
onsistingof32linkagegroupsfortheresistantmappingpopulationand37linkageg
roupsforthesusceptiblemappingpopulation.Themapshaveprovidedthebasisoff
uturemappingandmarker-assistedbreedingstudiesandstrategiesforthisbanana. |
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