Cultivation of auricularia polytricha mont. sacc (Black Jelly Mushroom) using oil palm wastes / Dang Lelamurni Abd. Razak
The increasing demand for continuous and quality supply of many types of mushrooms has drawn much attention and research to find potential substrate and domesticating the cultivation several species of edible mushrooms to suit local environmental conditions and resources. One of the popular mushr...
| Main Author: | |
|---|---|
| Format: | Thesis |
| Published: |
2013
|
| Subjects: | |
| Online Access: | http://pendeta.um.edu.my/client/default/search/detailnonmodal/ent:$002f$002fSD_ILS$002f988$002fSD_ILS:988336/ada?qu=Cultivation+of+auricularia+polytricha http://studentsrepo.um.edu.my/4142/1/FULL_THESIS.pdf |
| _version_ | 1848772573768187904 |
|---|---|
| author | Abd. Razak, Dang Lelamurni |
| author_facet | Abd. Razak, Dang Lelamurni |
| author_sort | Abd. Razak, Dang Lelamurni |
| building | UM Research Repository |
| collection | Online Access |
| description | The increasing demand for continuous and quality supply of many types of
mushrooms has drawn much attention and research to find potential substrate and
domesticating the cultivation several species of edible mushrooms to suit local
environmental conditions and resources. One of the popular mushrooms among
Malaysians due to its nutritional and medicinal values, Auricularia polytricha or Black
Jelly, offers high income to local growers due to its ability to grow and fruit in tropical
region such as Malaysia. The availability of a wide variety of agro-industrial byproducts
rich in lignocellulose and other materials required for mushroom growth
should be explored and used as the substrate ingredients for the cultivation of A.
polytricha as well as other mushroom species. This present study consisted of two main
parts, namely the study of A. polytricha spawn production and formulation of fruiting
substrates using oil palm wastes. The best substrate for production of spawn / inoculum
was determined by comparing A. polytricha mycelia growth on several types of grains.
It was found that crushed corn soaked for 4 hours, without adjusting its moisture content
and pH and with no addition of supplement or adjustment of C:N ratio was the optimum
substrate to support mycelia growth of A. polytricha at 6±1 mm/day growth rate. Corn
(uncrushed) supported the lowest mycelia growth rate, while soybean and peeled
soybean were deemed as unsuitable because of their high contamination rate. For
fruiting substrate, the potential of using agricultural and industrial wastes was evaluated
using several formulations of selected oil palm wastes combined with sawdust and also
supplemented with selected nitrogen sources. The best substrate formulations selected
were sawdust (SD) + oil palm frond (OPF) at a ratio of 90:10 supplemented with 15%
spent grain (SG) and sawdust (SD) + empty fruit bunch (EFB) at a ratio of 50:50
supplemented with 10% spent grain (SG) with mycelia growth rates of 8±1 mm/day and
iii
7±1 mm/day respectively. These two formulations were then subjected to different
moisture content levels (65%, 75% and 85%). Highest total fresh mushroom yield of
43.3% was obtained on SD+OPF (90:10) + 15% SG at 85% moisture content, followed
closely by SD+EFB (50:50) + 10% SG with 40.4% total yield, also at 85% moisture
content. Each of the substrate formulations at 85% moisture content gave the highest
biological efficiencies (BE) at 288.9% and 260.7% respectively. Both yield and
biological efficiency of A. polytricha on these two formulations were almost three times
higher when compared to sawdust substrate alone, thus proving the potential of these
formulations to improve yield of this mushroom. Analysis of nutrient content of A.
polytricha sporophores grown on SD+OPF (90:10) + 15% SG proved that this
mushroom contains eight essential amino acids at levels ranging from 0.007 to 0.05%.
In addition, cultivated A. polytricha in this study was found to be a valuable source of
protein, carbohydrates, crude fibre, magnesium, potassium, manganese and phosphorus
with values greater than 15% of the percentage daily portion based on RDA for each
nutrient. |
| first_indexed | 2025-11-14T13:28:40Z |
| format | Thesis |
| id | um-4142 |
| institution | University Malaya |
| institution_category | Local University |
| last_indexed | 2025-11-14T13:28:40Z |
| publishDate | 2013 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | um-41422014-09-30T02:18:03Z Cultivation of auricularia polytricha mont. sacc (Black Jelly Mushroom) using oil palm wastes / Dang Lelamurni Abd. Razak Abd. Razak, Dang Lelamurni Q Science (General) QH Natural history The increasing demand for continuous and quality supply of many types of mushrooms has drawn much attention and research to find potential substrate and domesticating the cultivation several species of edible mushrooms to suit local environmental conditions and resources. One of the popular mushrooms among Malaysians due to its nutritional and medicinal values, Auricularia polytricha or Black Jelly, offers high income to local growers due to its ability to grow and fruit in tropical region such as Malaysia. The availability of a wide variety of agro-industrial byproducts rich in lignocellulose and other materials required for mushroom growth should be explored and used as the substrate ingredients for the cultivation of A. polytricha as well as other mushroom species. This present study consisted of two main parts, namely the study of A. polytricha spawn production and formulation of fruiting substrates using oil palm wastes. The best substrate for production of spawn / inoculum was determined by comparing A. polytricha mycelia growth on several types of grains. It was found that crushed corn soaked for 4 hours, without adjusting its moisture content and pH and with no addition of supplement or adjustment of C:N ratio was the optimum substrate to support mycelia growth of A. polytricha at 6±1 mm/day growth rate. Corn (uncrushed) supported the lowest mycelia growth rate, while soybean and peeled soybean were deemed as unsuitable because of their high contamination rate. For fruiting substrate, the potential of using agricultural and industrial wastes was evaluated using several formulations of selected oil palm wastes combined with sawdust and also supplemented with selected nitrogen sources. The best substrate formulations selected were sawdust (SD) + oil palm frond (OPF) at a ratio of 90:10 supplemented with 15% spent grain (SG) and sawdust (SD) + empty fruit bunch (EFB) at a ratio of 50:50 supplemented with 10% spent grain (SG) with mycelia growth rates of 8±1 mm/day and iii 7±1 mm/day respectively. These two formulations were then subjected to different moisture content levels (65%, 75% and 85%). Highest total fresh mushroom yield of 43.3% was obtained on SD+OPF (90:10) + 15% SG at 85% moisture content, followed closely by SD+EFB (50:50) + 10% SG with 40.4% total yield, also at 85% moisture content. Each of the substrate formulations at 85% moisture content gave the highest biological efficiencies (BE) at 288.9% and 260.7% respectively. Both yield and biological efficiency of A. polytricha on these two formulations were almost three times higher when compared to sawdust substrate alone, thus proving the potential of these formulations to improve yield of this mushroom. Analysis of nutrient content of A. polytricha sporophores grown on SD+OPF (90:10) + 15% SG proved that this mushroom contains eight essential amino acids at levels ranging from 0.007 to 0.05%. In addition, cultivated A. polytricha in this study was found to be a valuable source of protein, carbohydrates, crude fibre, magnesium, potassium, manganese and phosphorus with values greater than 15% of the percentage daily portion based on RDA for each nutrient. 2013 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/4142/1/FULL_THESIS.pdf http://pendeta.um.edu.my/client/default/search/detailnonmodal/ent:$002f$002fSD_ILS$002f988$002fSD_ILS:988336/ada?qu=Cultivation+of+auricularia+polytricha Abd. Razak, Dang Lelamurni (2013) Cultivation of auricularia polytricha mont. sacc (Black Jelly Mushroom) using oil palm wastes / Dang Lelamurni Abd. Razak. Masters thesis, University of Malaya. http://studentsrepo.um.edu.my/4142/ |
| spellingShingle | Q Science (General) QH Natural history Abd. Razak, Dang Lelamurni Cultivation of auricularia polytricha mont. sacc (Black Jelly Mushroom) using oil palm wastes / Dang Lelamurni Abd. Razak |
| title | Cultivation of auricularia polytricha mont. sacc (Black Jelly Mushroom) using oil palm wastes / Dang Lelamurni Abd. Razak |
| title_full | Cultivation of auricularia polytricha mont. sacc (Black Jelly Mushroom) using oil palm wastes / Dang Lelamurni Abd. Razak |
| title_fullStr | Cultivation of auricularia polytricha mont. sacc (Black Jelly Mushroom) using oil palm wastes / Dang Lelamurni Abd. Razak |
| title_full_unstemmed | Cultivation of auricularia polytricha mont. sacc (Black Jelly Mushroom) using oil palm wastes / Dang Lelamurni Abd. Razak |
| title_short | Cultivation of auricularia polytricha mont. sacc (Black Jelly Mushroom) using oil palm wastes / Dang Lelamurni Abd. Razak |
| title_sort | cultivation of auricularia polytricha mont. sacc (black jelly mushroom) using oil palm wastes / dang lelamurni abd. razak |
| topic | Q Science (General) QH Natural history |
| url | http://pendeta.um.edu.my/client/default/search/detailnonmodal/ent:$002f$002fSD_ILS$002f988$002fSD_ILS:988336/ada?qu=Cultivation+of+auricularia+polytricha http://pendeta.um.edu.my/client/default/search/detailnonmodal/ent:$002f$002fSD_ILS$002f988$002fSD_ILS:988336/ada?qu=Cultivation+of+auricularia+polytricha http://studentsrepo.um.edu.my/4142/1/FULL_THESIS.pdf |