Association of DNA repair gene polymorphism (XRCC1) and oral cancer risk / Mohadeseh Hasanpourghadi
The main aim of the present study was to determine the distribution of XRCC1 Arg399Gln genotypes in oral cancer and non-oral cancer patients. This study was also to investigate the association between XRCC1 Arg399Gln genotypes and oral cancer risk and to compare the distribution of XRCC1 Arg399Gl...
| Summary: | The main aim of the present study was to determine the distribution of XRCC1 Arg399Gln
genotypes in oral cancer and non-oral cancer patients. This study was also to investigate the
association between XRCC1 Arg399Gln genotypes and oral cancer risk and to compare the
distribution of XRCC1 Arg399Gln genotypes among different ethnic groups in Malaysia.
This case-control study involved a total of 209 cases of oral squamous cell
carcinoma (OSCC) patients and 212 controls with neither any trace of cancers nor any
family history of cancer. Mean age of the OSCC patients for cases was 61.34 ± 14.01 years,
while for the control the mean age was 45.56 ± 12 years. About 62.07% (131) and 37.03%
(78) were females and males respectively for cases and 54.02% (115) and 45.08% (97)
females and males respectively for controls. Determination of XRCC1 Arg399Gln
genotypes was done using genomic DNA from blood samples. The final genotypes were
determined using PCR and PCR-RFLP where 3 genotypes were recognized; namely the
normal/wild-type (Arg/Arg), the 2 polymorphic genotypes namely the heterozygote
(Arg/Gln) and the homozygote (Gln/Gln).
The results revealed that the distribution of XRCC1 Arg399Gln polymorphism (Arg/Gln;
Gln/Gln) was 65.1% for cases and 58.5% for controls. When the polymorphisms were
considered separately, the distribution of Arg/Gln among the cases was 48.8% and among
controls was 41%. The distribution of Gln/Gln among cases and controls were almost
similar which is 16.3% and 17.5% respectively.
The Chi square test revealed either individually or in combination that there was no
significant association between XRCC1 Arg399Gln genotypes and oral cancer risk. Similarly, there was no significant difference in the distribution of XRCC1 Arg399Gln
genotypes when analyzed either individually (p=0.617) or in combination (p=0.641)
between three different ethnic groups.
In conclusion, there was a higher distribution of XRCC1 Arg399Gln polymorphism
in cases as compared to control. There was no association between XRCC1 polymorphism
and oral cancer risk and there was no significant difference observed in XRCC1 genotype
distribution in the different ethnic groups. |
|---|