Fluorescene imaging of mouse sperm / Noorain H.
The protocol for detecting acrosome in mouse sperm was developed by using two types of fluorescent dyes namely fluorescein conjugated Pisum sativum agglutinin (FITC-PSA) and rhodamine conjugated Lens culinaris agglutinin (TRITC-LCA). The results showed that mean percentages of acrosome intact sperm...
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| Format: | Thesis |
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2012
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| Online Access: | http://studentsrepo.um.edu.my/3481/ http://studentsrepo.um.edu.my/3481/1/Chapter_6%2D2amac2010FINAL.pdf |
| _version_ | 1848772424172044288 |
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| author | Nooraain, H. |
| author_facet | Nooraain, H. |
| author_sort | Nooraain, H. |
| building | UM Research Repository |
| collection | Online Access |
| description | The protocol for detecting acrosome in mouse sperm was developed by using two types of fluorescent dyes namely fluorescein conjugated Pisum sativum agglutinin (FITC-PSA) and rhodamine conjugated Lens culinaris agglutinin (TRITC-LCA). The results showed that mean percentages of acrosome intact sperm incubated in vitro were considerably low. After one-hour incubation there were 43.20±2.83%, 31.81±8.44% and 30.90±4.15% for ICR, C57/6J and F1 sperm, respectively. Intact acrosome in ICR sperm was significantly reduced (p<0.05) to 16.95±4.23% after two-hour incubation, however, insignificant reduction (p>0.05) was found for C57BL/6J and F1 sperm, which were 30.03±2.06% and 20.93±3.81%, respectively. The results showed that both dyes FITC-PSA and TRITC-LCA suitably stained the mouse sperm and produced insignificant difference (p>0.05) in the percentages of acrosome intact sperm, which were 40.03±4.20% and 49.79±4.63%, respectively. One-hour incubation was adequate to capacitate sperm in vitro. Low acrosome intact due to premature acrosome reaction during extended period of incubation may compromise in vitro fertilisation rates. |
| first_indexed | 2025-11-14T13:26:17Z |
| format | Thesis |
| id | um-3481 |
| institution | University Malaya |
| institution_category | Local University |
| last_indexed | 2025-11-14T13:26:17Z |
| publishDate | 2012 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | um-34812013-09-06T04:48:44Z Fluorescene imaging of mouse sperm / Noorain H. Nooraain, H. QL Zoology The protocol for detecting acrosome in mouse sperm was developed by using two types of fluorescent dyes namely fluorescein conjugated Pisum sativum agglutinin (FITC-PSA) and rhodamine conjugated Lens culinaris agglutinin (TRITC-LCA). The results showed that mean percentages of acrosome intact sperm incubated in vitro were considerably low. After one-hour incubation there were 43.20±2.83%, 31.81±8.44% and 30.90±4.15% for ICR, C57/6J and F1 sperm, respectively. Intact acrosome in ICR sperm was significantly reduced (p<0.05) to 16.95±4.23% after two-hour incubation, however, insignificant reduction (p>0.05) was found for C57BL/6J and F1 sperm, which were 30.03±2.06% and 20.93±3.81%, respectively. The results showed that both dyes FITC-PSA and TRITC-LCA suitably stained the mouse sperm and produced insignificant difference (p>0.05) in the percentages of acrosome intact sperm, which were 40.03±4.20% and 49.79±4.63%, respectively. One-hour incubation was adequate to capacitate sperm in vitro. Low acrosome intact due to premature acrosome reaction during extended period of incubation may compromise in vitro fertilisation rates. 2012-08-08 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/3481/1/Chapter_6%2D2amac2010FINAL.pdf Nooraain, H. (2012) Fluorescene imaging of mouse sperm / Noorain H. PhD thesis, University of Malaya. http://studentsrepo.um.edu.my/3481/ |
| spellingShingle | QL Zoology Nooraain, H. Fluorescene imaging of mouse sperm / Noorain H. |
| title | Fluorescene imaging of mouse sperm / Noorain H. |
| title_full | Fluorescene imaging of mouse sperm / Noorain H. |
| title_fullStr | Fluorescene imaging of mouse sperm / Noorain H. |
| title_full_unstemmed | Fluorescene imaging of mouse sperm / Noorain H. |
| title_short | Fluorescene imaging of mouse sperm / Noorain H. |
| title_sort | fluorescene imaging of mouse sperm / noorain h. |
| topic | QL Zoology |
| url | http://studentsrepo.um.edu.my/3481/ http://studentsrepo.um.edu.my/3481/1/Chapter_6%2D2amac2010FINAL.pdf |