Genotoxic and hematological effects of endocrine disruptive chemicals, bisphenol a and nonylphenol, on Nile tilapia (Oreochromis niloticus) / Muhammad Faris Sahardin
This study was performed to evaluate the genotoxicity and hematology effects of single and binary exposure of bisphenol A and nonylphenol towards Oreochromis niloticus. O. niloticus was singly exposed to bisphenol A at concentration of 2 mg/l and nonylphenol at concentration of 0.04 mg/l in 16 L...
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| Format: | Thesis |
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2020
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| Online Access: | http://studentsrepo.um.edu.my/12319/ http://studentsrepo.um.edu.my/12319/2/Muhammad_Faris.pdf http://studentsrepo.um.edu.my/12319/1/Muhammad_Faris.pdf |
| Summary: | This study was performed to evaluate the genotoxicity and hematology effects of single
and binary exposure of bisphenol A and nonylphenol towards Oreochromis niloticus. O.
niloticus was singly exposed to bisphenol A at concentration of 2 mg/l and nonylphenol
at concentration of 0.04 mg/l in 16 L glass tank. O. niloticus was also synergistically
exposed to the both concentration of 2 mg/l of bisphenol A and 0.04 mg/l of nonylphenol.
Four fishes were randomly used for four exposures after two weeks acclimatization. All
of them were exposed to the compound for 96 hours without water change prior
observation. Fishes were anaesthetized and sacrificed for blood for the analysis of
micronucleus, comet assay and hematology. The observation showed significant
difference in the micronuclei frequency and nuclear abnormalities frequency in
micronucleus test when they were singly exposed to 0.04 mg/l of nonylphenol. The
obvious strand breakage of supercoiled duplex DNA had been determined in the comet
assay analysis in single exposure of 0.04 mg/l of nonylphenol compared to others. Result
from complete blood count also showed nonylphenol exposure at concentration 0.04 mg/l
caused significant difference in some blood parameters when compared to the control.
Based on this study, it was proved that single exposure of nonylphenol contributed
stronger effect to O. niloticus compared to single exposure of bisphenol A and binary exposure of both chemicals.
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