Differential expression of mucin in salivary gland tumours / Nurul Inaas Mahamad Apandi
Introduction: Varied alterations in types of secreted mucin may affect the regulation of cell growth, immune response and adhesion of cell. These changes may indirectly contribute to the ability of tumour invasion and metastasis. However, the expression of mucins in salivary gland tumours has not...
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| Format: | Thesis |
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2020
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| Online Access: | http://studentsrepo.um.edu.my/12087/ http://studentsrepo.um.edu.my/12087/4/inaas.pdf |
| Summary: | Introduction: Varied alterations in types of secreted mucin may affect the regulation
of cell growth, immune response and adhesion of cell. These changes may indirectly
contribute to the ability of tumour invasion and metastasis. However, the expression of
mucins in salivary gland tumours has not been explored in depth. Objectives: To
investigate expressions of mucin in the salivary gland tumour microenvironment and
make comparisons between benign and malignant, and minor and major salivary gland
tumours. Methods: Special stains were used to stain neutral mucin (Periodic acid
Schiff), sialomucin (Alcian Blue) and sulfomucin (Aldehyde Fuschin) within tissues
from 6 normal salivary glands and salivary gland tumours including 31 pleomorphic
adenoma (PA), 27 mucoepidermoid carcinoma (MEC) and 15 adenoid cystic
carcinoma (AdCC). Statistical analyses to compare mucin expression in these salivary
gland tumours were done using Chi-square tests. Results: Sialomucin was the most
expressed mucin in all salivary gland tumours regardless of origin. A significant
difference was observed in mucin expression between benign and malignant salivary
gland tumours, in which PA showed 3 times significantly higher expression of
sialomucin compared to MEC and AdCC (p=0.028). PAs of major gland origin
showed 42 times significantly higher expression of sialomucin compared to
PAs of minor gland (p=0.000). Conclusion: Alcian blue was the best special
stain to visualize mucin elements in salivary gland tumours. Sialomucin content in PA
of major glands was vastly increased from that in minor glands. The degree of
sialomucin expression may play a role in diagnosis of borderline salivary gland
tumours. |
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