Expression of KI-67, FSCNI and TNFRSF 12A in histologically non-involved mucosal surgical margins as predictive markers for local relapse in oral squamous cell carcinoma / Nur Fauziani Zainul Abidin
Introduction: The presence tumour-related genetic alterations in histologically noninvolved mucosal surgical margins was linked to development of local relapse in OSCC. Aims: The present study aimed to determine the association between expression of Ki67, FSCN1 and TNFRSF12A in histologically non-i...
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| Format: | Thesis |
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2020
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| Online Access: | http://studentsrepo.um.edu.my/12086/ http://studentsrepo.um.edu.my/12086/4/fauziani.pdf |
| _version_ | 1848774559106334720 |
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| author | Nur Fauziani, Zainul Abidin |
| author_facet | Nur Fauziani, Zainul Abidin |
| author_sort | Nur Fauziani, Zainul Abidin |
| building | UM Research Repository |
| collection | Online Access |
| description | Introduction: The presence tumour-related genetic alterations in histologically noninvolved mucosal surgical margins was linked to development of local relapse in OSCC.
Aims: The present study aimed to determine the association between expression of Ki67, FSCN1 and TNFRSF12A in histologically non-involved mucosal surgical margins of
OSCC patients and local relapse. Materials and methods: The study involved a total of
34 OSCC cases including 15 cases of patients who had relapse within the 5 years followup period and 19 cases of patients who did not had relapse. Two-stage sampling was
carried out to select the margins’ site, followed by the representative sections from the
selected site. All the selected sections were subjected to FSCN1, TNFRSF12A and Ki-67
immunostaining. The immunostaining for FSCN1 and TNFRSF12A were evaluated by a
semi-quantitative approach (HSCORE), while Ki-67 immuno-positivity was scored by
the percentage of positive staining cells in randomly selected fields (Labelling index).
The correlation of FSCN1, TNFRSF12A and Ki-67 expressions and clinicopathological
parameters with OSCC relapse were analysed by Chi-square test. Binary logistic
regression was performed to predict the relationship of OSCC relapse and the
significantly associated parameters. Results: Our results showed that expression of
FSCN1, TNFRSF12A and Ki-67 in histologically non-involved mucosal surgical margins
was weak to moderate in both study and control groups. OSCC relapse in this study was
significantly associated with patients’ age (p < 0.001), pattern of invasion (p = 0.007),
Chinese ethnicity (p =0.013), presence of epithelial dysplasia (p < 0.001), and alcohol
consumption habits (p = 0.025). Age was the only predictor of relapse from the binary
logistic regression analysis with an 11-fold risk of OSCC relapse noted in relation to
patients who were aged above 57.5 years. Conclusion: The expression of FSCN1,
TNFRSF12A and Ki-67 in histologically non-involved mucosal surgical margins in the
iv
present study was not associated with relapse in OSCC. However, patients’ age may be a
reliable predictor for OSCC relapse. |
| first_indexed | 2025-11-14T14:00:13Z |
| format | Thesis |
| id | um-12086 |
| institution | University Malaya |
| institution_category | Local University |
| last_indexed | 2025-11-14T14:00:13Z |
| publishDate | 2020 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | um-120862023-01-02T22:42:51Z Expression of KI-67, FSCNI and TNFRSF 12A in histologically non-involved mucosal surgical margins as predictive markers for local relapse in oral squamous cell carcinoma / Nur Fauziani Zainul Abidin Nur Fauziani, Zainul Abidin RK Dentistry Introduction: The presence tumour-related genetic alterations in histologically noninvolved mucosal surgical margins was linked to development of local relapse in OSCC. Aims: The present study aimed to determine the association between expression of Ki67, FSCN1 and TNFRSF12A in histologically non-involved mucosal surgical margins of OSCC patients and local relapse. Materials and methods: The study involved a total of 34 OSCC cases including 15 cases of patients who had relapse within the 5 years followup period and 19 cases of patients who did not had relapse. Two-stage sampling was carried out to select the margins’ site, followed by the representative sections from the selected site. All the selected sections were subjected to FSCN1, TNFRSF12A and Ki-67 immunostaining. The immunostaining for FSCN1 and TNFRSF12A were evaluated by a semi-quantitative approach (HSCORE), while Ki-67 immuno-positivity was scored by the percentage of positive staining cells in randomly selected fields (Labelling index). The correlation of FSCN1, TNFRSF12A and Ki-67 expressions and clinicopathological parameters with OSCC relapse were analysed by Chi-square test. Binary logistic regression was performed to predict the relationship of OSCC relapse and the significantly associated parameters. Results: Our results showed that expression of FSCN1, TNFRSF12A and Ki-67 in histologically non-involved mucosal surgical margins was weak to moderate in both study and control groups. OSCC relapse in this study was significantly associated with patients’ age (p < 0.001), pattern of invasion (p = 0.007), Chinese ethnicity (p =0.013), presence of epithelial dysplasia (p < 0.001), and alcohol consumption habits (p = 0.025). Age was the only predictor of relapse from the binary logistic regression analysis with an 11-fold risk of OSCC relapse noted in relation to patients who were aged above 57.5 years. Conclusion: The expression of FSCN1, TNFRSF12A and Ki-67 in histologically non-involved mucosal surgical margins in the iv present study was not associated with relapse in OSCC. However, patients’ age may be a reliable predictor for OSCC relapse. 2020 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/12086/4/fauziani.pdf Nur Fauziani, Zainul Abidin (2020) Expression of KI-67, FSCNI and TNFRSF 12A in histologically non-involved mucosal surgical margins as predictive markers for local relapse in oral squamous cell carcinoma / Nur Fauziani Zainul Abidin. Masters thesis, Universiti Malaya. http://studentsrepo.um.edu.my/12086/ |
| spellingShingle | RK Dentistry Nur Fauziani, Zainul Abidin Expression of KI-67, FSCNI and TNFRSF 12A in histologically non-involved mucosal surgical margins as predictive markers for local relapse in oral squamous cell carcinoma / Nur Fauziani Zainul Abidin |
| title | Expression of KI-67, FSCNI and TNFRSF 12A in histologically non-involved mucosal surgical margins as predictive markers for local relapse in oral squamous cell carcinoma / Nur Fauziani Zainul Abidin |
| title_full | Expression of KI-67, FSCNI and TNFRSF 12A in histologically non-involved mucosal surgical margins as predictive markers for local relapse in oral squamous cell carcinoma / Nur Fauziani Zainul Abidin |
| title_fullStr | Expression of KI-67, FSCNI and TNFRSF 12A in histologically non-involved mucosal surgical margins as predictive markers for local relapse in oral squamous cell carcinoma / Nur Fauziani Zainul Abidin |
| title_full_unstemmed | Expression of KI-67, FSCNI and TNFRSF 12A in histologically non-involved mucosal surgical margins as predictive markers for local relapse in oral squamous cell carcinoma / Nur Fauziani Zainul Abidin |
| title_short | Expression of KI-67, FSCNI and TNFRSF 12A in histologically non-involved mucosal surgical margins as predictive markers for local relapse in oral squamous cell carcinoma / Nur Fauziani Zainul Abidin |
| title_sort | expression of ki-67, fscni and tnfrsf 12a in histologically non-involved mucosal surgical margins as predictive markers for local relapse in oral squamous cell carcinoma / nur fauziani zainul abidin |
| topic | RK Dentistry |
| url | http://studentsrepo.um.edu.my/12086/ http://studentsrepo.um.edu.my/12086/4/fauziani.pdf |