Detection of subgingival periodonto-pathogens and inflammaory cytokines in Malaysian adults with chronic periodontitis / Nursolehah Shahruddin
Objectives: In periodontitis, the interaction between lipopolysaccharide from gram-negative bacteria with host cells initiates the secretion of cytokines such as interleukin-1 (IL-1), interleukin-6 (IL-6) and tumour necrosis factor-α (TNF-α). The aim of this study was to assess the frequency of d...
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| Format: | Thesis |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://studentsrepo.um.edu.my/11697/ http://studentsrepo.um.edu.my/11697/4/nursolehah.pdf |
| _version_ | 1848774453020852224 |
|---|---|
| author | Nursolehah, Shahruddin |
| author_facet | Nursolehah, Shahruddin |
| author_sort | Nursolehah, Shahruddin |
| building | UM Research Repository |
| collection | Online Access |
| description | Objectives: In periodontitis, the interaction between lipopolysaccharide from gram-negative
bacteria with host cells initiates the secretion of cytokines such as interleukin-1 (IL-1),
interleukin-6 (IL-6) and tumour necrosis factor-α (TNF-α). The aim of this study was to
assess the frequency of detection of subgingival microbiota Porphyromonas gingivalis (P.
gingivalis), Tannerella forsythia (T. forsythia), Prevotella intermedia (P. intermedia) and
Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) and the levels of IL6, IL-17, resistin and TNF-α among selected population of Malaysian chronic periodontitis
(CP) subjects and to assess their association with periodontal parameters.
Methods: A total of 167 CP and 134 healthy subjects were recruited. Visible plaque index
(VPI), gingival bleeding index (GBI), probing pocket depths (PPD) and clinical attachment
levels (CAL) were recorded. Subgingival plaque and blood were sampled from all subjects.
Quantification of resistin, IL-17, IL-6 and TNF-ɑ was performed by ELISA. Real time PCR
(qPCR) was used to frequency of detection and quantitate of P. gingivalis, T. forsythia, P.
intermedia and A. actinomycetemcomitans.
Results: Prevalence and mean counts of P. gingivalis, T. forsythia, P. intermedia and A.
actinomycetemcomitans were higher in CP subjects [(94.50%, 24.66×105 cells/μl), (92.10%,
7.70×105 cells/μl), (72.60%, 6.61×105 cells/μl), (7.30%, 0.14×105 cells/μl) respectively] (p
<0.05) compared to healthy controls [(73.70%, 8.32×105 cells/μl), (62.00%, 4.75×105
cells/μl), (58.40%, 5.17×105 cells/μl), (not determined (ND)) respectively]. Similarly level of
resistin, IL-17, IL-6 and TNF-ɑ levels were higher in CP subjects [(100%, 274620 pg/ml),
iv
(100%, 727.13pg/ml), (100%, 26.84pg/ml), (100%, 54.91pg/ml) respectively] (p <0.001)
compared to healthy controls ((94.90%, 22.12pg/ml), (45.30%, 0.19pg/ml), (30.70%,
0.006pg/ml), (ND) respectively). CP was found as the only confounder that influenced all
clinical parameters, periodontal pathogens and inflammatory mediators. Additional
confounders were CAL for P. gingivalis, PPD for T. forsythia and, VPI and CAL for IL-17.
In the CP group, there was a positive but weak correlation between T. forsythia and IL-17
(r=0.189, p=0.016) and A. actinomycetemcomitans with TNF-α (r=0.166, p<0.033). Healthy
group showed no significant correlation between periodontal pathogens and all inflammatory
mediators.
Conclusion: Adults with CP were found to have significantly higher prevalence and mean
counts of P. gingivalis, T. forsythia, P. intermedia and A. actinomycetemcomitans and
inflammatory mediators (resistin, IL-17, IL-6 and TNF-α) as compared to healthy controls.
P. gingivalis was found to be positively associated with mean CAL while IL-17 was
negatively associated with mean CAL. T. forsythia was positively associated with mean
PPD. There was a weak but significant correlation between T. forsythia with IL-17 and A.
actinomycetemcomitans with TNF-ɑ. |
| first_indexed | 2025-11-14T13:58:32Z |
| format | Thesis |
| id | um-11697 |
| institution | University Malaya |
| institution_category | Local University |
| last_indexed | 2025-11-14T13:58:32Z |
| publishDate | 2019 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | um-116972022-01-05T22:22:13Z Detection of subgingival periodonto-pathogens and inflammaory cytokines in Malaysian adults with chronic periodontitis / Nursolehah Shahruddin Nursolehah, Shahruddin RK Dentistry Objectives: In periodontitis, the interaction between lipopolysaccharide from gram-negative bacteria with host cells initiates the secretion of cytokines such as interleukin-1 (IL-1), interleukin-6 (IL-6) and tumour necrosis factor-α (TNF-α). The aim of this study was to assess the frequency of detection of subgingival microbiota Porphyromonas gingivalis (P. gingivalis), Tannerella forsythia (T. forsythia), Prevotella intermedia (P. intermedia) and Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) and the levels of IL6, IL-17, resistin and TNF-α among selected population of Malaysian chronic periodontitis (CP) subjects and to assess their association with periodontal parameters. Methods: A total of 167 CP and 134 healthy subjects were recruited. Visible plaque index (VPI), gingival bleeding index (GBI), probing pocket depths (PPD) and clinical attachment levels (CAL) were recorded. Subgingival plaque and blood were sampled from all subjects. Quantification of resistin, IL-17, IL-6 and TNF-ɑ was performed by ELISA. Real time PCR (qPCR) was used to frequency of detection and quantitate of P. gingivalis, T. forsythia, P. intermedia and A. actinomycetemcomitans. Results: Prevalence and mean counts of P. gingivalis, T. forsythia, P. intermedia and A. actinomycetemcomitans were higher in CP subjects [(94.50%, 24.66×105 cells/μl), (92.10%, 7.70×105 cells/μl), (72.60%, 6.61×105 cells/μl), (7.30%, 0.14×105 cells/μl) respectively] (p <0.05) compared to healthy controls [(73.70%, 8.32×105 cells/μl), (62.00%, 4.75×105 cells/μl), (58.40%, 5.17×105 cells/μl), (not determined (ND)) respectively]. Similarly level of resistin, IL-17, IL-6 and TNF-ɑ levels were higher in CP subjects [(100%, 274620 pg/ml), iv (100%, 727.13pg/ml), (100%, 26.84pg/ml), (100%, 54.91pg/ml) respectively] (p <0.001) compared to healthy controls ((94.90%, 22.12pg/ml), (45.30%, 0.19pg/ml), (30.70%, 0.006pg/ml), (ND) respectively). CP was found as the only confounder that influenced all clinical parameters, periodontal pathogens and inflammatory mediators. Additional confounders were CAL for P. gingivalis, PPD for T. forsythia and, VPI and CAL for IL-17. In the CP group, there was a positive but weak correlation between T. forsythia and IL-17 (r=0.189, p=0.016) and A. actinomycetemcomitans with TNF-α (r=0.166, p<0.033). Healthy group showed no significant correlation between periodontal pathogens and all inflammatory mediators. Conclusion: Adults with CP were found to have significantly higher prevalence and mean counts of P. gingivalis, T. forsythia, P. intermedia and A. actinomycetemcomitans and inflammatory mediators (resistin, IL-17, IL-6 and TNF-α) as compared to healthy controls. P. gingivalis was found to be positively associated with mean CAL while IL-17 was negatively associated with mean CAL. T. forsythia was positively associated with mean PPD. There was a weak but significant correlation between T. forsythia with IL-17 and A. actinomycetemcomitans with TNF-ɑ. 2019 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/11697/4/nursolehah.pdf Nursolehah, Shahruddin (2019) Detection of subgingival periodonto-pathogens and inflammaory cytokines in Malaysian adults with chronic periodontitis / Nursolehah Shahruddin. Masters thesis, Universiti Malaya. http://studentsrepo.um.edu.my/11697/ |
| spellingShingle | RK Dentistry Nursolehah, Shahruddin Detection of subgingival periodonto-pathogens and inflammaory cytokines in Malaysian adults with chronic periodontitis / Nursolehah Shahruddin |
| title | Detection of subgingival periodonto-pathogens and inflammaory cytokines in Malaysian adults with chronic periodontitis / Nursolehah Shahruddin |
| title_full | Detection of subgingival periodonto-pathogens and inflammaory cytokines in Malaysian adults with chronic periodontitis / Nursolehah Shahruddin |
| title_fullStr | Detection of subgingival periodonto-pathogens and inflammaory cytokines in Malaysian adults with chronic periodontitis / Nursolehah Shahruddin |
| title_full_unstemmed | Detection of subgingival periodonto-pathogens and inflammaory cytokines in Malaysian adults with chronic periodontitis / Nursolehah Shahruddin |
| title_short | Detection of subgingival periodonto-pathogens and inflammaory cytokines in Malaysian adults with chronic periodontitis / Nursolehah Shahruddin |
| title_sort | detection of subgingival periodonto-pathogens and inflammaory cytokines in malaysian adults with chronic periodontitis / nursolehah shahruddin |
| topic | RK Dentistry |
| url | http://studentsrepo.um.edu.my/11697/ http://studentsrepo.um.edu.my/11697/4/nursolehah.pdf |