Expression of KI-67, Cornulin and ISG15 in noninvolved mucosal surgical margins as predictive markers for relapse in oral squamous cell carcinoma (OSCC) / Lew Huai Lin
Introduction: Relapse in OSCC is often observed in histologically non-involved surgical margins. This indicates possible presence of field alteration in the non-involved surgical margins, which in turn leads to local recurrence or second primary tumour. The identification of specific biomarkers t...
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| Format: | Thesis |
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2019
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| Online Access: | http://studentsrepo.um.edu.my/11215/ http://studentsrepo.um.edu.my/11215/4/huai_lin.pdf |
| _version_ | 1848774326254305280 |
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| author | Lew, Huai Lin |
| author_facet | Lew, Huai Lin |
| author_sort | Lew, Huai Lin |
| building | UM Research Repository |
| collection | Online Access |
| description | Introduction: Relapse in OSCC is often observed in histologically non-involved surgical
margins. This indicates possible presence of field alteration in the non-involved surgical
margins, which in turn leads to local recurrence or second primary tumour. The
identification of specific biomarkers that could predict relapse of OSCC would help the
clinicians in treatment planning for patients. Objectives: The objectives of this study
were to evaluate the expression of Ki-67, Cornulin and ISG15 in the non-involved
surgical margins and its association with clinicopathological prognosticators and relapse
of OSCC. Methods: Immunohistochemistry was used in staining of non-involved
mucosal surgical margins from study (relapse) group (n = 23), control (non-relapse) group
(n = 32) and normal oral mucosa (n = 5) with Ki-67, Cornulin and ISG15. Association
between expression of markers with clinicopathological prognosticators and relapse in
OSCC was analysed statistically using Chi-square tests. Binary logistic regression
analysis was used to determine predictors of relapse in OSCC. Results: In the study
group, significant low expression of Cornulin (p = 0.032) and ISG15 (p = 0.047) was
observed. Low expression of Cornulin was also significantly associated with relapse (p =
0.004) of OSCC and primary tumours involving non-tongue sites (p = 0.013). Surgical
margins exhibiting high expression of Ki-67 was significantly reduced in female patients
(p = 0.041). Clinicopathological prognosticators such as age above 57.5 years (p < 0.001),
Chinese ethnicity (p = 0.009), Indian ethnicity (p = 0.007), alcohol use (p = 0.025),
epithelial dysplasia in surgical margins (p = 0.045) and type III and IV pattern of invasion
of tumour (p = 0.007) were significantly associated with relapse of OSCC. Binary logistic
regression analysis showed decreased expression of Cornulin (p = 0.018) and increased
patient’s age (p = 0.008) were predictors of relapse in OSCC, with 34-fold risk and 18-
iv
fold risk, respectively. Conclusions: Relapse of OSCC could be predicted by decreased
expression of Cornulin in the non-involved surgical margins. Validation of role of Ki-67
and ISG15 in predicting relapse of OSCC would require larger cohorts. Taken together,
Cornulin as a predictor in relapse of OSCC was suggested.
Keywords: OSCC, Relapse, Ki-67, Cornulin, ISG15 |
| first_indexed | 2025-11-14T13:56:31Z |
| format | Thesis |
| id | um-11215 |
| institution | University Malaya |
| institution_category | Local University |
| last_indexed | 2025-11-14T13:56:31Z |
| publishDate | 2019 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | um-112152022-01-02T18:22:45Z Expression of KI-67, Cornulin and ISG15 in noninvolved mucosal surgical margins as predictive markers for relapse in oral squamous cell carcinoma (OSCC) / Lew Huai Lin Lew, Huai Lin RK Dentistry Introduction: Relapse in OSCC is often observed in histologically non-involved surgical margins. This indicates possible presence of field alteration in the non-involved surgical margins, which in turn leads to local recurrence or second primary tumour. The identification of specific biomarkers that could predict relapse of OSCC would help the clinicians in treatment planning for patients. Objectives: The objectives of this study were to evaluate the expression of Ki-67, Cornulin and ISG15 in the non-involved surgical margins and its association with clinicopathological prognosticators and relapse of OSCC. Methods: Immunohistochemistry was used in staining of non-involved mucosal surgical margins from study (relapse) group (n = 23), control (non-relapse) group (n = 32) and normal oral mucosa (n = 5) with Ki-67, Cornulin and ISG15. Association between expression of markers with clinicopathological prognosticators and relapse in OSCC was analysed statistically using Chi-square tests. Binary logistic regression analysis was used to determine predictors of relapse in OSCC. Results: In the study group, significant low expression of Cornulin (p = 0.032) and ISG15 (p = 0.047) was observed. Low expression of Cornulin was also significantly associated with relapse (p = 0.004) of OSCC and primary tumours involving non-tongue sites (p = 0.013). Surgical margins exhibiting high expression of Ki-67 was significantly reduced in female patients (p = 0.041). Clinicopathological prognosticators such as age above 57.5 years (p < 0.001), Chinese ethnicity (p = 0.009), Indian ethnicity (p = 0.007), alcohol use (p = 0.025), epithelial dysplasia in surgical margins (p = 0.045) and type III and IV pattern of invasion of tumour (p = 0.007) were significantly associated with relapse of OSCC. Binary logistic regression analysis showed decreased expression of Cornulin (p = 0.018) and increased patient’s age (p = 0.008) were predictors of relapse in OSCC, with 34-fold risk and 18- iv fold risk, respectively. Conclusions: Relapse of OSCC could be predicted by decreased expression of Cornulin in the non-involved surgical margins. Validation of role of Ki-67 and ISG15 in predicting relapse of OSCC would require larger cohorts. Taken together, Cornulin as a predictor in relapse of OSCC was suggested. Keywords: OSCC, Relapse, Ki-67, Cornulin, ISG15 2019 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/11215/4/huai_lin.pdf Lew, Huai Lin (2019) Expression of KI-67, Cornulin and ISG15 in noninvolved mucosal surgical margins as predictive markers for relapse in oral squamous cell carcinoma (OSCC) / Lew Huai Lin. Masters thesis, Universiti Malaya. http://studentsrepo.um.edu.my/11215/ |
| spellingShingle | RK Dentistry Lew, Huai Lin Expression of KI-67, Cornulin and ISG15 in noninvolved mucosal surgical margins as predictive markers for relapse in oral squamous cell carcinoma (OSCC) / Lew Huai Lin |
| title | Expression of KI-67, Cornulin and ISG15 in noninvolved mucosal surgical margins as predictive markers for relapse in oral squamous cell carcinoma (OSCC) / Lew Huai Lin |
| title_full | Expression of KI-67, Cornulin and ISG15 in noninvolved mucosal surgical margins as predictive markers for relapse in oral squamous cell carcinoma (OSCC) / Lew Huai Lin |
| title_fullStr | Expression of KI-67, Cornulin and ISG15 in noninvolved mucosal surgical margins as predictive markers for relapse in oral squamous cell carcinoma (OSCC) / Lew Huai Lin |
| title_full_unstemmed | Expression of KI-67, Cornulin and ISG15 in noninvolved mucosal surgical margins as predictive markers for relapse in oral squamous cell carcinoma (OSCC) / Lew Huai Lin |
| title_short | Expression of KI-67, Cornulin and ISG15 in noninvolved mucosal surgical margins as predictive markers for relapse in oral squamous cell carcinoma (OSCC) / Lew Huai Lin |
| title_sort | expression of ki-67, cornulin and isg15 in noninvolved mucosal surgical margins as predictive markers for relapse in oral squamous cell carcinoma (oscc) / lew huai lin |
| topic | RK Dentistry |
| url | http://studentsrepo.um.edu.my/11215/ http://studentsrepo.um.edu.my/11215/4/huai_lin.pdf |