Optimisation of biomass and exopolysaccharide production in wild Serbian Ganoderma lucidum strain BGF4A1 using response surface methodology / Nurul Amirah Hassan
A wild-Serbian medicinal mushroom Ganoderma lucidum strain BGF4A1 (GLSB) was isolated from Mount Avala, Serbia and morphologically identified based on its brown-liquorish cap and woody stipe. Molecularly, GLSB (642 bp) was sequenced and found to be 99% similar to the Serbian-originated G. lucidum st...
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| Format: | Thesis |
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2019
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| Online Access: | http://studentsrepo.um.edu.my/11024/ http://studentsrepo.um.edu.my/11024/2/Nurul_Amirah.pdf http://studentsrepo.um.edu.my/11024/1/Nurul_Amirah.pdf |
| _version_ | 1848774276712235008 |
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| author | Nurul Amirah , Hassan |
| author_facet | Nurul Amirah , Hassan |
| author_sort | Nurul Amirah , Hassan |
| building | UM Research Repository |
| collection | Online Access |
| description | A wild-Serbian medicinal mushroom Ganoderma lucidum strain BGF4A1 (GLSB) was isolated from Mount Avala, Serbia and morphologically identified based on its brown-liquorish cap and woody stipe. Molecularly, GLSB (642 bp) was sequenced and found to be 99% similar to the Serbian-originated G. lucidum strain BEOFB 434 and G. lucidum strain BEOFB 431. The isolate belongs to the G. lucidum species as the sequence dissimilarities (Knuc) value between both sequences of the same fungal species was 0.001. In submerged-liquid fermentation, biomass and exopolysaccharide (EPS) production of GLSB was optimised using response surface methodology. The interactions between three variables: initial pH (4–6), temperature (20C–30C), and glucose concentration (10 g/L–50 g/L) were analysed using a central composite design. An analysis of variance revealed that the model was significant for all parameters investigated (p < 0.05). Temperature and glucose concentration were found to significantly influence mycelial biomass production, whereas for EPS production only glucose concentration had a significant effect. The model for biomass and EPS was validated by implementing the optimised conditions (pH 5.26, 50 g/L glucose, and 30C) and was found to generate the highest biomass (3.12 g/L) and EPS (1.96 g/L). An efficient EPS-biomass production blueprint was thus established using optimised parameters for large-scale cultivation of Serbian G. lucidum strains.
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| first_indexed | 2025-11-14T13:55:44Z |
| format | Thesis |
| id | um-11024 |
| institution | University Malaya |
| institution_category | Local University |
| last_indexed | 2025-11-14T13:55:44Z |
| publishDate | 2019 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | um-110242020-02-21T00:50:10Z Optimisation of biomass and exopolysaccharide production in wild Serbian Ganoderma lucidum strain BGF4A1 using response surface methodology / Nurul Amirah Hassan Nurul Amirah , Hassan Q Science (General) QH301 Biology A wild-Serbian medicinal mushroom Ganoderma lucidum strain BGF4A1 (GLSB) was isolated from Mount Avala, Serbia and morphologically identified based on its brown-liquorish cap and woody stipe. Molecularly, GLSB (642 bp) was sequenced and found to be 99% similar to the Serbian-originated G. lucidum strain BEOFB 434 and G. lucidum strain BEOFB 431. The isolate belongs to the G. lucidum species as the sequence dissimilarities (Knuc) value between both sequences of the same fungal species was 0.001. In submerged-liquid fermentation, biomass and exopolysaccharide (EPS) production of GLSB was optimised using response surface methodology. The interactions between three variables: initial pH (4–6), temperature (20C–30C), and glucose concentration (10 g/L–50 g/L) were analysed using a central composite design. An analysis of variance revealed that the model was significant for all parameters investigated (p < 0.05). Temperature and glucose concentration were found to significantly influence mycelial biomass production, whereas for EPS production only glucose concentration had a significant effect. The model for biomass and EPS was validated by implementing the optimised conditions (pH 5.26, 50 g/L glucose, and 30C) and was found to generate the highest biomass (3.12 g/L) and EPS (1.96 g/L). An efficient EPS-biomass production blueprint was thus established using optimised parameters for large-scale cultivation of Serbian G. lucidum strains. 2019-08 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/11024/2/Nurul_Amirah.pdf application/pdf http://studentsrepo.um.edu.my/11024/1/Nurul_Amirah.pdf Nurul Amirah , Hassan (2019) Optimisation of biomass and exopolysaccharide production in wild Serbian Ganoderma lucidum strain BGF4A1 using response surface methodology / Nurul Amirah Hassan. Masters thesis, University of Malaya. http://studentsrepo.um.edu.my/11024/ |
| spellingShingle | Q Science (General) QH301 Biology Nurul Amirah , Hassan Optimisation of biomass and exopolysaccharide production in wild Serbian Ganoderma lucidum strain BGF4A1 using response surface methodology / Nurul Amirah Hassan |
| title | Optimisation of biomass and exopolysaccharide production in wild Serbian Ganoderma lucidum strain BGF4A1 using response surface methodology / Nurul Amirah Hassan |
| title_full | Optimisation of biomass and exopolysaccharide production in wild Serbian Ganoderma lucidum strain BGF4A1 using response surface methodology / Nurul Amirah Hassan |
| title_fullStr | Optimisation of biomass and exopolysaccharide production in wild Serbian Ganoderma lucidum strain BGF4A1 using response surface methodology / Nurul Amirah Hassan |
| title_full_unstemmed | Optimisation of biomass and exopolysaccharide production in wild Serbian Ganoderma lucidum strain BGF4A1 using response surface methodology / Nurul Amirah Hassan |
| title_short | Optimisation of biomass and exopolysaccharide production in wild Serbian Ganoderma lucidum strain BGF4A1 using response surface methodology / Nurul Amirah Hassan |
| title_sort | optimisation of biomass and exopolysaccharide production in wild serbian ganoderma lucidum strain bgf4a1 using response surface methodology / nurul amirah hassan |
| topic | Q Science (General) QH301 Biology |
| url | http://studentsrepo.um.edu.my/11024/ http://studentsrepo.um.edu.my/11024/2/Nurul_Amirah.pdf http://studentsrepo.um.edu.my/11024/1/Nurul_Amirah.pdf |