Secretion of RANTES and interleukin-8 by the human gastric epithelial cell line (MKN45) through modulation with cytokines
In this vitro study, the secretion of interleukin-8 (IL-8) and RANTES proteins by the MKN45 gastric epithetial cell line was examined using the ELISA technique after the cells had been stimulated with pro-inflammatory cytokines, I,e,II, I "α" ,TNF-α and IFN-γ, The chemokines demonstrated a...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
penerbit ukm
2003
|
| Online Access: | http://journalarticle.ukm.my/946/ http://journalarticle.ukm.my/946/1/jurnal2.pdf |
| _version_ | 1848809365733113856 |
|---|---|
| author | Norina Abdullah, Jordon N.J, Kolios G., Colloins A.J, |
| author_facet | Norina Abdullah, Jordon N.J, Kolios G., Colloins A.J, |
| author_sort | Norina Abdullah, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | In this vitro study, the secretion of interleukin-8 (IL-8) and RANTES proteins by the MKN45 gastric epithetial cell line was examined using the ELISA technique after the cells had been stimulated with pro-inflammatory cytokines, I,e,II, I "α" ,TNF-α and IFN-γ, The chemokines demonstrated a timedependent increase in concentration after stimulation with the various cytokines, alone or in combination, IL-I α was the weakest stimuli while a combination of IL-I α, TNF-α and IFN-γ provided the strongest stimulation. The immunomodulatory effects of IL-4 and IL-13 on the chemokine protein secretion were also studied. Stimulation of the cells with IL-4 and IL-13 for an hour at relatively high concentrations (10 and 30 ng/ml) prior to stimulation with the combination of IL-I α, TNF-α and IFN –γ inhibited the secretion of RANTES protein but did not inhibit the secretion of IL-8 mRNA of protwin. This inplied that the chemokines might have different inbitiory pathways. IL-4 and IL-13 played an important role in decreasing certain inflammatory chemokines and this might have an important effect in decreasing gastric inflammation. |
| first_indexed | 2025-11-14T23:13:27Z |
| format | Article |
| id | ukm-946 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T23:13:27Z |
| publishDate | 2003 |
| publisher | penerbit ukm |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ukm-9462016-12-14T06:28:23Z http://journalarticle.ukm.my/946/ Secretion of RANTES and interleukin-8 by the human gastric epithelial cell line (MKN45) through modulation with cytokines Norina Abdullah, Jordon N.J, Kolios G., Colloins A.J, In this vitro study, the secretion of interleukin-8 (IL-8) and RANTES proteins by the MKN45 gastric epithetial cell line was examined using the ELISA technique after the cells had been stimulated with pro-inflammatory cytokines, I,e,II, I "α" ,TNF-α and IFN-γ, The chemokines demonstrated a timedependent increase in concentration after stimulation with the various cytokines, alone or in combination, IL-I α was the weakest stimuli while a combination of IL-I α, TNF-α and IFN-γ provided the strongest stimulation. The immunomodulatory effects of IL-4 and IL-13 on the chemokine protein secretion were also studied. Stimulation of the cells with IL-4 and IL-13 for an hour at relatively high concentrations (10 and 30 ng/ml) prior to stimulation with the combination of IL-I α, TNF-α and IFN –γ inhibited the secretion of RANTES protein but did not inhibit the secretion of IL-8 mRNA of protwin. This inplied that the chemokines might have different inbitiory pathways. IL-4 and IL-13 played an important role in decreasing certain inflammatory chemokines and this might have an important effect in decreasing gastric inflammation. penerbit ukm 2003-07 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/946/1/jurnal2.pdf Norina Abdullah, and Jordon N.J, and Kolios G., and Colloins A.J, (2003) Secretion of RANTES and interleukin-8 by the human gastric epithelial cell line (MKN45) through modulation with cytokines. Jurnal Sains Kesihatan Malaysia, 1 . pp. 9-21. ISSN 1675-8161 http://www.fskb.ukm.my/fskbold/jurnal |
| spellingShingle | Norina Abdullah, Jordon N.J, Kolios G., Colloins A.J, Secretion of RANTES and interleukin-8 by the human gastric epithelial cell line (MKN45) through modulation with cytokines |
| title | Secretion of RANTES and interleukin-8 by the human gastric epithelial cell line (MKN45) through modulation with cytokines |
| title_full | Secretion of RANTES and interleukin-8 by the human gastric epithelial cell line (MKN45) through modulation with cytokines |
| title_fullStr | Secretion of RANTES and interleukin-8 by the human gastric epithelial cell line (MKN45) through modulation with cytokines |
| title_full_unstemmed | Secretion of RANTES and interleukin-8 by the human gastric epithelial cell line (MKN45) through modulation with cytokines |
| title_short | Secretion of RANTES and interleukin-8 by the human gastric epithelial cell line (MKN45) through modulation with cytokines |
| title_sort | secretion of rantes and interleukin-8 by the human gastric epithelial cell line (mkn45) through modulation with cytokines |
| url | http://journalarticle.ukm.my/946/ http://journalarticle.ukm.my/946/ http://journalarticle.ukm.my/946/1/jurnal2.pdf |