Detection of BCR/ABL gene in chronic Myeloid Leukaemia: comparison of Fluorescence in situ Hybridisation (FISH), conventional Cytogenetics and Polymerase Chain Reaction (PCR) techniques
The reciprocal translocation t(9;22)(q34;q11) which gives rise to the Philadelphia (Ph1) chromosome and BCR/ABL fusion gene, plays a pivotal role in the diagnosis and pathogenesis of chronic myeloid leukemia (CML). In this study, we evaluated the role of fluorescence in situ hybridisation (FISH) in...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit UKM
2006
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| Online Access: | http://journalarticle.ukm.my/1829/ http://journalarticle.ukm.my/1829/1/02._5-13_%28MH_006%29.pdf |
| _version_ | 1848809669811765248 |
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| author | Reena RMZ, Julia Munchar MJ, Salwati S,, Hamidah NH, Zubaidah Z, Sharifah NA, Cheong SK, |
| author_facet | Reena RMZ, Julia Munchar MJ, Salwati S,, Hamidah NH, Zubaidah Z, Sharifah NA, Cheong SK, |
| author_sort | Reena RMZ, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | The reciprocal translocation t(9;22)(q34;q11) which gives rise to the Philadelphia (Ph1) chromosome and BCR/ABL fusion gene, plays a pivotal role in the diagnosis and pathogenesis of chronic myeloid leukemia (CML). In this study, we evaluated the role of fluorescence in situ hybridisation (FISH) in detecting the BCR/ABL rearrangement in CML patients. The sensitivity, specificity and detection rate of BCR/ABL gene using FISH, PCR and conventional cytogenetics (karyotyping) methods were also compared. 18 bone marrow samples of patients with clinically diagnosed CML and suspected of CML were collected. The sensitivity, specificity and positive predictive values of FISH were altogether |
| first_indexed | 2025-11-14T23:18:17Z |
| format | Article |
| id | ukm-1829 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T23:18:17Z |
| publishDate | 2006 |
| publisher | Penerbit UKM |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ukm-18292016-12-14T06:30:18Z http://journalarticle.ukm.my/1829/ Detection of BCR/ABL gene in chronic Myeloid Leukaemia: comparison of Fluorescence in situ Hybridisation (FISH), conventional Cytogenetics and Polymerase Chain Reaction (PCR) techniques Reena RMZ, Julia Munchar MJ, Salwati S,, Hamidah NH, Zubaidah Z, Sharifah NA, Cheong SK, The reciprocal translocation t(9;22)(q34;q11) which gives rise to the Philadelphia (Ph1) chromosome and BCR/ABL fusion gene, plays a pivotal role in the diagnosis and pathogenesis of chronic myeloid leukemia (CML). In this study, we evaluated the role of fluorescence in situ hybridisation (FISH) in detecting the BCR/ABL rearrangement in CML patients. The sensitivity, specificity and detection rate of BCR/ABL gene using FISH, PCR and conventional cytogenetics (karyotyping) methods were also compared. 18 bone marrow samples of patients with clinically diagnosed CML and suspected of CML were collected. The sensitivity, specificity and positive predictive values of FISH were altogether Penerbit UKM 2006 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/1829/1/02._5-13_%28MH_006%29.pdf Reena RMZ, and Julia Munchar MJ, and Salwati S,, and Hamidah NH, and Zubaidah Z, and Sharifah NA, and Cheong SK, (2006) Detection of BCR/ABL gene in chronic Myeloid Leukaemia: comparison of Fluorescence in situ Hybridisation (FISH), conventional Cytogenetics and Polymerase Chain Reaction (PCR) techniques. Medicine & Health, 1 (1). pp. 5-13. ISSN 1823-2140 http://www.ppukm.ukm.my/ukmmcjournal/index.php |
| spellingShingle | Reena RMZ, Julia Munchar MJ, Salwati S,, Hamidah NH, Zubaidah Z, Sharifah NA, Cheong SK, Detection of BCR/ABL gene in chronic Myeloid Leukaemia: comparison of Fluorescence in situ Hybridisation (FISH), conventional Cytogenetics and Polymerase Chain Reaction (PCR) techniques |
| title | Detection of BCR/ABL gene in chronic Myeloid Leukaemia: comparison of Fluorescence in situ Hybridisation (FISH), conventional Cytogenetics and Polymerase Chain Reaction (PCR) techniques |
| title_full | Detection of BCR/ABL gene in chronic Myeloid Leukaemia: comparison of Fluorescence in situ Hybridisation (FISH), conventional Cytogenetics and Polymerase Chain Reaction (PCR) techniques |
| title_fullStr | Detection of BCR/ABL gene in chronic Myeloid Leukaemia: comparison of Fluorescence in situ Hybridisation (FISH), conventional Cytogenetics and Polymerase Chain Reaction (PCR) techniques |
| title_full_unstemmed | Detection of BCR/ABL gene in chronic Myeloid Leukaemia: comparison of Fluorescence in situ Hybridisation (FISH), conventional Cytogenetics and Polymerase Chain Reaction (PCR) techniques |
| title_short | Detection of BCR/ABL gene in chronic Myeloid Leukaemia: comparison of Fluorescence in situ Hybridisation (FISH), conventional Cytogenetics and Polymerase Chain Reaction (PCR) techniques |
| title_sort | detection of bcr/abl gene in chronic myeloid leukaemia: comparison of fluorescence in situ hybridisation (fish), conventional cytogenetics and polymerase chain reaction (pcr) techniques |
| url | http://journalarticle.ukm.my/1829/ http://journalarticle.ukm.my/1829/ http://journalarticle.ukm.my/1829/1/02._5-13_%28MH_006%29.pdf |