Menskala naik satu proses fermentasi berdasarkan pekali pemindahan isipadu (kLa)oksigen tetap
The production of the enzyme cytochrome P-450 by Saccharomyces cerevisiae N.C.Y.C. 754 in batch fermentations using a coconut water based medium was scaled-up from shake flask to a 2-liter stirred - tank fermenter scale using the method of constant volumetric transfer coefficient (kLa) of oxygen....
| Main Authors: | , , , |
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| Format: | Article |
| Published: |
1990
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| Online Access: | http://journalarticle.ukm.my/1285/ |
| _version_ | 1848809480681160704 |
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| author | Ahmad Jaril Asis, Zulaikha Paidi, A. Winkler, Michael Jailani Salihon, |
| author_facet | Ahmad Jaril Asis, Zulaikha Paidi, A. Winkler, Michael Jailani Salihon, |
| author_sort | Ahmad Jaril Asis, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | The production of the enzyme cytochrome P-450 by Saccharomyces
cerevisiae N.C.Y.C. 754 in batch fermentations using a coconut water based medium
was scaled-up from shake flask to a 2-liter stirred - tank fermenter scale using the
method of constant volumetric transfer coefficient (kLa) of oxygen.. kLa values were
derived by fitting the mass transfer equation to the data of dissolved oxygen tension
(DOT) versus time on computer using the Simplex Method with two unknowns,
namely kLa and the electrode mass transfer coefficient (kap) of oxygen. The stirrer
speed (RPM) and the air flowrate, (A) in the 2-liter fermenter that produced the value
of kLa found in the optimised conditions in shake flask was approximated by trial and
error. A fermentation run in the 2-liter fermenter using these values of RPM and A
gave an enzyme yield comparable to that achieved in shake-flasks |
| first_indexed | 2025-11-14T23:15:17Z |
| format | Article |
| id | ukm-1285 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T23:15:17Z |
| publishDate | 1990 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ukm-12852011-10-11T03:45:15Z http://journalarticle.ukm.my/1285/ Menskala naik satu proses fermentasi berdasarkan pekali pemindahan isipadu (kLa)oksigen tetap Ahmad Jaril Asis, Zulaikha Paidi, A. Winkler, Michael Jailani Salihon, The production of the enzyme cytochrome P-450 by Saccharomyces cerevisiae N.C.Y.C. 754 in batch fermentations using a coconut water based medium was scaled-up from shake flask to a 2-liter stirred - tank fermenter scale using the method of constant volumetric transfer coefficient (kLa) of oxygen.. kLa values were derived by fitting the mass transfer equation to the data of dissolved oxygen tension (DOT) versus time on computer using the Simplex Method with two unknowns, namely kLa and the electrode mass transfer coefficient (kap) of oxygen. The stirrer speed (RPM) and the air flowrate, (A) in the 2-liter fermenter that produced the value of kLa found in the optimised conditions in shake flask was approximated by trial and error. A fermentation run in the 2-liter fermenter using these values of RPM and A gave an enzyme yield comparable to that achieved in shake-flasks 1990 Article PeerReviewed Ahmad Jaril Asis, and Zulaikha Paidi, and A. Winkler, Michael and Jailani Salihon, (1990) Menskala naik satu proses fermentasi berdasarkan pekali pemindahan isipadu (kLa)oksigen tetap. Jurnal Kejuruteraan, 2 . http://www.ukm.my/jkukm/index.php/jkukm |
| spellingShingle | Ahmad Jaril Asis, Zulaikha Paidi, A. Winkler, Michael Jailani Salihon, Menskala naik satu proses fermentasi berdasarkan pekali pemindahan isipadu (kLa)oksigen tetap |
| title | Menskala naik satu proses fermentasi berdasarkan pekali pemindahan isipadu
(kLa)oksigen tetap
|
| title_full | Menskala naik satu proses fermentasi berdasarkan pekali pemindahan isipadu
(kLa)oksigen tetap
|
| title_fullStr | Menskala naik satu proses fermentasi berdasarkan pekali pemindahan isipadu
(kLa)oksigen tetap
|
| title_full_unstemmed | Menskala naik satu proses fermentasi berdasarkan pekali pemindahan isipadu
(kLa)oksigen tetap
|
| title_short | Menskala naik satu proses fermentasi berdasarkan pekali pemindahan isipadu
(kLa)oksigen tetap
|
| title_sort | menskala naik satu proses fermentasi berdasarkan pekali pemindahan isipadu
(kla)oksigen tetap |
| url | http://journalarticle.ukm.my/1285/ http://journalarticle.ukm.my/1285/ |