Determination of benzoic acid in fermented fish products using HPLC / Norlida Baharudin

Food fermentation is a widespread traditional technology which has been improved into more sophisticated industries. The fermented fish products have two characteristics, solid and semi-solid. Belacan is the example of solid and the examples of semi-solid are budu and cencaluk. Benzoic acid was u...

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Main Author: Baharudin, Norlida
Format: Thesis
Language:English
Published: 2009
Subjects:
Online Access:https://ir.uitm.edu.my/id/eprint/764/
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author Baharudin, Norlida
author_facet Baharudin, Norlida
author_sort Baharudin, Norlida
building UiTM Institutional Repository
collection Online Access
description Food fermentation is a widespread traditional technology which has been improved into more sophisticated industries. The fermented fish products have two characteristics, solid and semi-solid. Belacan is the example of solid and the examples of semi-solid are budu and cencaluk. Benzoic acid was used in fermented fish products as a preservative. It is used to prevent growth of yeast and mould, being also effective against a wide range of bacteria. It was analysed using High Performance Liquid Chromatography (HPLC) using two mobile phase (MP) systems. The mobile phase system (MP I) used are methanol-glacial acetic acid-water (35: 5: 60) with detection of 245 nm and mobile phase system (MP II) are methanol-0.01M ammonium acetate buffer with detection of 235 nm. For mobile phase I, the amount of benzoic in cencaluk was found to be 2609 ppm and the amount of benzoic acid in budu and belacan was found to be unavailable for detection. While for mobile phase II, the amount of benzoic acid was found to be absent at the all samples. The level of benzoic acid in cencaluk was apparently higher than the permissible level of 750 ppm according to Malaysian Food and Agricultural Import Regulations and Standards, Country Report (2004).
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institution Universiti Teknologi MARA
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language English
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publishDate 2009
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spelling uitm-7642023-01-13T02:41:17Z https://ir.uitm.edu.my/id/eprint/764/ Determination of benzoic acid in fermented fish products using HPLC / Norlida Baharudin Baharudin, Norlida QD Chemistry Food fermentation is a widespread traditional technology which has been improved into more sophisticated industries. The fermented fish products have two characteristics, solid and semi-solid. Belacan is the example of solid and the examples of semi-solid are budu and cencaluk. Benzoic acid was used in fermented fish products as a preservative. It is used to prevent growth of yeast and mould, being also effective against a wide range of bacteria. It was analysed using High Performance Liquid Chromatography (HPLC) using two mobile phase (MP) systems. The mobile phase system (MP I) used are methanol-glacial acetic acid-water (35: 5: 60) with detection of 245 nm and mobile phase system (MP II) are methanol-0.01M ammonium acetate buffer with detection of 235 nm. For mobile phase I, the amount of benzoic in cencaluk was found to be 2609 ppm and the amount of benzoic acid in budu and belacan was found to be unavailable for detection. While for mobile phase II, the amount of benzoic acid was found to be absent at the all samples. The level of benzoic acid in cencaluk was apparently higher than the permissible level of 750 ppm according to Malaysian Food and Agricultural Import Regulations and Standards, Country Report (2004). 2009 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/764/2/764.pdf Baharudin, Norlida (2009) Determination of benzoic acid in fermented fish products using HPLC / Norlida Baharudin. (2009) UNSPECIFIED thesis, thesis, Universiti Teknologi MARA. <http://terminalib.uitm.edu.my/764.pdf>
spellingShingle QD Chemistry
Baharudin, Norlida
Determination of benzoic acid in fermented fish products using HPLC / Norlida Baharudin
title Determination of benzoic acid in fermented fish products using HPLC / Norlida Baharudin
title_full Determination of benzoic acid in fermented fish products using HPLC / Norlida Baharudin
title_fullStr Determination of benzoic acid in fermented fish products using HPLC / Norlida Baharudin
title_full_unstemmed Determination of benzoic acid in fermented fish products using HPLC / Norlida Baharudin
title_short Determination of benzoic acid in fermented fish products using HPLC / Norlida Baharudin
title_sort determination of benzoic acid in fermented fish products using hplc / norlida baharudin
topic QD Chemistry
url https://ir.uitm.edu.my/id/eprint/764/