Cytotoxicity and DNA cleavage studies of salicylhydroxamic acid and its Ni(II), Cu(II) and Zn(II) complexes / Nor Haslini Wahid
Ni((II), Cu(II) and Zn(II) complexes of salicylhydroxamic acid have been synthesized and characterized by elemental analysis, FT-IR, 1HNMR spectroscopy, magnetic moment, melting point determination and conductivity measurement data. The spectral data suggests that the salicylhydroxamic acid act a...
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| Format: | Student Project |
| Language: | English |
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Faculty of Applied Sciences
2009
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| Subjects: | |
| Online Access: | https://ir.uitm.edu.my/id/eprint/763/ |
| _version_ | 1848802396153577472 |
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| author | Wahid, Nor Haslini |
| author_facet | Wahid, Nor Haslini |
| author_sort | Wahid, Nor Haslini |
| building | UiTM Institutional Repository |
| collection | Online Access |
| description | Ni((II), Cu(II) and Zn(II) complexes of salicylhydroxamic acid have been synthesized
and characterized by elemental analysis, FT-IR, 1HNMR spectroscopy, magnetic
moment, melting point determination and conductivity measurement data. The spectral
data suggests that the salicylhydroxamic acid act as a bidentate ligand and is coordinated
to the metal ion through the hydroxamate group. The interaction of the salicylhydroxamic
acid ligand and its complexes with double stranded supercoiled DNA was studied using
agarose gel electrophoresis. From the DNA cleavage studies, it was found that the
supercoiled structure of the bacterial DNA was maintained indicating that all compounds
had no negative effect on bacterial DNA. The ligand and complexes were also subjected
to in vitro cytotoxicity test using Chang liver cells. Treatment of Zn(II) complex to these
cells have shown that the IC50 value is at 62 μM. This shows that Zn(II) complex had
toxic effect on Chang liver cells. A decrease in cells viability indicates that the cells were
stopping actively growing and dividing. But the ligand and other complexes tested were
found to be non-toxic. |
| first_indexed | 2025-11-14T21:22:41Z |
| format | Student Project |
| id | uitm-763 |
| institution | Universiti Teknologi MARA |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T21:22:41Z |
| publishDate | 2009 |
| publisher | Faculty of Applied Sciences |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uitm-7632018-09-20T04:43:23Z https://ir.uitm.edu.my/id/eprint/763/ Cytotoxicity and DNA cleavage studies of salicylhydroxamic acid and its Ni(II), Cu(II) and Zn(II) complexes / Nor Haslini Wahid Wahid, Nor Haslini QD Chemistry Ni((II), Cu(II) and Zn(II) complexes of salicylhydroxamic acid have been synthesized and characterized by elemental analysis, FT-IR, 1HNMR spectroscopy, magnetic moment, melting point determination and conductivity measurement data. The spectral data suggests that the salicylhydroxamic acid act as a bidentate ligand and is coordinated to the metal ion through the hydroxamate group. The interaction of the salicylhydroxamic acid ligand and its complexes with double stranded supercoiled DNA was studied using agarose gel electrophoresis. From the DNA cleavage studies, it was found that the supercoiled structure of the bacterial DNA was maintained indicating that all compounds had no negative effect on bacterial DNA. The ligand and complexes were also subjected to in vitro cytotoxicity test using Chang liver cells. Treatment of Zn(II) complex to these cells have shown that the IC50 value is at 62 μM. This shows that Zn(II) complex had toxic effect on Chang liver cells. A decrease in cells viability indicates that the cells were stopping actively growing and dividing. But the ligand and other complexes tested were found to be non-toxic. Faculty of Applied Sciences 2009 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/763/1/PPb_NOR%20HASLINI%20WAHID%20AS%2009_5%20P01.pdf Wahid, Nor Haslini (2009) Cytotoxicity and DNA cleavage studies of salicylhydroxamic acid and its Ni(II), Cu(II) and Zn(II) complexes / Nor Haslini Wahid. (2009) [Student Project] <http://terminalib.uitm.edu.my/763.pdf> (Unpublished) |
| spellingShingle | QD Chemistry Wahid, Nor Haslini Cytotoxicity and DNA cleavage studies of salicylhydroxamic acid and its Ni(II), Cu(II) and Zn(II) complexes / Nor Haslini Wahid |
| title | Cytotoxicity and DNA cleavage studies of salicylhydroxamic acid and its Ni(II), Cu(II) and Zn(II) complexes / Nor Haslini Wahid |
| title_full | Cytotoxicity and DNA cleavage studies of salicylhydroxamic acid and its Ni(II), Cu(II) and Zn(II) complexes / Nor Haslini Wahid |
| title_fullStr | Cytotoxicity and DNA cleavage studies of salicylhydroxamic acid and its Ni(II), Cu(II) and Zn(II) complexes / Nor Haslini Wahid |
| title_full_unstemmed | Cytotoxicity and DNA cleavage studies of salicylhydroxamic acid and its Ni(II), Cu(II) and Zn(II) complexes / Nor Haslini Wahid |
| title_short | Cytotoxicity and DNA cleavage studies of salicylhydroxamic acid and its Ni(II), Cu(II) and Zn(II) complexes / Nor Haslini Wahid |
| title_sort | cytotoxicity and dna cleavage studies of salicylhydroxamic acid and its ni(ii), cu(ii) and zn(ii) complexes / nor haslini wahid |
| topic | QD Chemistry |
| url | https://ir.uitm.edu.my/id/eprint/763/ |