Antibacterial and antioxidant activities of the seeds of artocarpus heterophyllus / Eumi Nazera Mohd Nazir

The Jackfruit tree, which also known as Artocarpus heterophyllus, is widespread tree all over Asia. The seed of fruit from this species was studied. The seed was extracted using soxhlet extractor with hexane, dichloromethane (DCM) and methanol. The extraction yield obtained were 1.9%, 2.6% and 2.3%...

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Main Author: Mohd Nazir, Eumi Nazera
Format: Student Project
Language:English
Published: 2017
Subjects:
Online Access:https://ir.uitm.edu.my/id/eprint/35129/
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author Mohd Nazir, Eumi Nazera
author_facet Mohd Nazir, Eumi Nazera
author_sort Mohd Nazir, Eumi Nazera
building UiTM Institutional Repository
collection Online Access
description The Jackfruit tree, which also known as Artocarpus heterophyllus, is widespread tree all over Asia. The seed of fruit from this species was studied. The seed was extracted using soxhlet extractor with hexane, dichloromethane (DCM) and methanol. The extraction yield obtained were 1.9%, 2.6% and 2.3% of extract methanol, DCM and hexane respectively. Thin Layer Chromatography (TLC) analysis displayed a suitable solvent system to separate the component within each extract. The identified solvent system suitable for methanol extract was DCM:chloroform:hexane (3:2:2), for DCM extract, the identified solvent system was chloroform: hexane (2:1), while, for hexane extract, the identified solvent system was toluene:ethyl acetate:formic acid (7:1:1). The crude extracts was then undergone several test which was antioxidant and antibacterial. The antioxidant test was done using DPPH Radical Scavenging Activity method and the antibacterial test was done using Disc Diffusion method. It was found that all sample extracts had a low antioxidant activity with the percentage radical scavenging activity lower than 22% compared to ascorbic acid. For the antibacterial test, methanol extract exhibited a highest inhibition zone of 16 mm against Salmonella sp., 14 mm against E.coli, and, 18 mm against B.subtillis and S. aureus.
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format Student Project
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institution Universiti Teknologi MARA
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language English
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publishDate 2017
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spelling uitm-351292020-10-13T06:24:23Z https://ir.uitm.edu.my/id/eprint/35129/ Antibacterial and antioxidant activities of the seeds of artocarpus heterophyllus / Eumi Nazera Mohd Nazir Mohd Nazir, Eumi Nazera Laboratories. General works Extraction (Chemistry) Microorganisms The Jackfruit tree, which also known as Artocarpus heterophyllus, is widespread tree all over Asia. The seed of fruit from this species was studied. The seed was extracted using soxhlet extractor with hexane, dichloromethane (DCM) and methanol. The extraction yield obtained were 1.9%, 2.6% and 2.3% of extract methanol, DCM and hexane respectively. Thin Layer Chromatography (TLC) analysis displayed a suitable solvent system to separate the component within each extract. The identified solvent system suitable for methanol extract was DCM:chloroform:hexane (3:2:2), for DCM extract, the identified solvent system was chloroform: hexane (2:1), while, for hexane extract, the identified solvent system was toluene:ethyl acetate:formic acid (7:1:1). The crude extracts was then undergone several test which was antioxidant and antibacterial. The antioxidant test was done using DPPH Radical Scavenging Activity method and the antibacterial test was done using Disc Diffusion method. It was found that all sample extracts had a low antioxidant activity with the percentage radical scavenging activity lower than 22% compared to ascorbic acid. For the antibacterial test, methanol extract exhibited a highest inhibition zone of 16 mm against Salmonella sp., 14 mm against E.coli, and, 18 mm against B.subtillis and S. aureus. 2017 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/35129/1/35129.pdf Mohd Nazir, Eumi Nazera (2017) Antibacterial and antioxidant activities of the seeds of artocarpus heterophyllus / Eumi Nazera Mohd Nazir. (2017) [Student Project] (Unpublished)
spellingShingle Laboratories. General works
Extraction (Chemistry)
Microorganisms
Mohd Nazir, Eumi Nazera
Antibacterial and antioxidant activities of the seeds of artocarpus heterophyllus / Eumi Nazera Mohd Nazir
title Antibacterial and antioxidant activities of the seeds of artocarpus heterophyllus / Eumi Nazera Mohd Nazir
title_full Antibacterial and antioxidant activities of the seeds of artocarpus heterophyllus / Eumi Nazera Mohd Nazir
title_fullStr Antibacterial and antioxidant activities of the seeds of artocarpus heterophyllus / Eumi Nazera Mohd Nazir
title_full_unstemmed Antibacterial and antioxidant activities of the seeds of artocarpus heterophyllus / Eumi Nazera Mohd Nazir
title_short Antibacterial and antioxidant activities of the seeds of artocarpus heterophyllus / Eumi Nazera Mohd Nazir
title_sort antibacterial and antioxidant activities of the seeds of artocarpus heterophyllus / eumi nazera mohd nazir
topic Laboratories. General works
Extraction (Chemistry)
Microorganisms
url https://ir.uitm.edu.my/id/eprint/35129/