Antibacterial and antioxidant activities of the leaves crude extracts of garcinia mangostana linn / Nurrul Atika Masrol

This study was undertaken to find the antibacterial and antioxidant activities from crude extracts of Garcinia mangostana leaves. Cold extraction technique was used in order to get the crude extract by using petroleum ether, chloroform and ethanol as solvents. The crude extracts then investigated fo...

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Main Author: Masrol, Nurrul Atika
Format: Student Project
Language:English
Published: 2017
Subjects:
Online Access:https://ir.uitm.edu.my/id/eprint/35096/
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author Masrol, Nurrul Atika
author_facet Masrol, Nurrul Atika
author_sort Masrol, Nurrul Atika
building UiTM Institutional Repository
collection Online Access
description This study was undertaken to find the antibacterial and antioxidant activities from crude extracts of Garcinia mangostana leaves. Cold extraction technique was used in order to get the crude extract by using petroleum ether, chloroform and ethanol as solvents. The crude extracts then investigated for separation in Thin Layer Chromatography (TLC) analysis. The best solvent systems for three crude extracts were mixture of chloroform and dichloromethane. It shows the best separation for the three crude extracts. Antibacterial activity was conducted by using Gram positive bacteria (Bacillus subtilis and Staphylococcus aureus) and Gram negative bacteria (Escherichia coli and Salmonella typhi,). Petroleum ether and ethanol extract showed active inhibition against four bacteria while chloroform extract active only at Bacillus subtilis. The total antioxidant assay was conducted by using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Ethanol extract showed highest inhibition percentage among the crude extracts. Ethanol extract exhibited significant scavenging activity when compared to ascorbic acid indicates its potential as antioxidant.
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format Student Project
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institution Universiti Teknologi MARA
institution_category Local University
language English
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publishDate 2017
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spelling uitm-350962020-10-15T15:06:02Z https://ir.uitm.edu.my/id/eprint/35096/ Antibacterial and antioxidant activities of the leaves crude extracts of garcinia mangostana linn / Nurrul Atika Masrol Masrol, Nurrul Atika Laboratories. General works Extraction (Chemistry) Antioxidants This study was undertaken to find the antibacterial and antioxidant activities from crude extracts of Garcinia mangostana leaves. Cold extraction technique was used in order to get the crude extract by using petroleum ether, chloroform and ethanol as solvents. The crude extracts then investigated for separation in Thin Layer Chromatography (TLC) analysis. The best solvent systems for three crude extracts were mixture of chloroform and dichloromethane. It shows the best separation for the three crude extracts. Antibacterial activity was conducted by using Gram positive bacteria (Bacillus subtilis and Staphylococcus aureus) and Gram negative bacteria (Escherichia coli and Salmonella typhi,). Petroleum ether and ethanol extract showed active inhibition against four bacteria while chloroform extract active only at Bacillus subtilis. The total antioxidant assay was conducted by using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Ethanol extract showed highest inhibition percentage among the crude extracts. Ethanol extract exhibited significant scavenging activity when compared to ascorbic acid indicates its potential as antioxidant. 2017 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/35096/1/35096.pdf Masrol, Nurrul Atika (2017) Antibacterial and antioxidant activities of the leaves crude extracts of garcinia mangostana linn / Nurrul Atika Masrol. (2017) [Student Project] (Unpublished)
spellingShingle Laboratories. General works
Extraction (Chemistry)
Antioxidants
Masrol, Nurrul Atika
Antibacterial and antioxidant activities of the leaves crude extracts of garcinia mangostana linn / Nurrul Atika Masrol
title Antibacterial and antioxidant activities of the leaves crude extracts of garcinia mangostana linn / Nurrul Atika Masrol
title_full Antibacterial and antioxidant activities of the leaves crude extracts of garcinia mangostana linn / Nurrul Atika Masrol
title_fullStr Antibacterial and antioxidant activities of the leaves crude extracts of garcinia mangostana linn / Nurrul Atika Masrol
title_full_unstemmed Antibacterial and antioxidant activities of the leaves crude extracts of garcinia mangostana linn / Nurrul Atika Masrol
title_short Antibacterial and antioxidant activities of the leaves crude extracts of garcinia mangostana linn / Nurrul Atika Masrol
title_sort antibacterial and antioxidant activities of the leaves crude extracts of garcinia mangostana linn / nurrul atika masrol
topic Laboratories. General works
Extraction (Chemistry)
Antioxidants
url https://ir.uitm.edu.my/id/eprint/35096/