Antioxidant and antimicrobial activities of piper betle / Norsyahida Yasir

Piper betleleaves was extracted by soxhlet extraction method withhexane, chloroform and methanol solvent. The yield of crude extraction of hexane, chloroform and methanol is 9.53%, 13.47% and 7.86% respectively. All three types of crude extraction was undergo Thin Layer Chromatography (TLC) analysis...

Full description

Bibliographic Details
Main Author: Yasir, Norsyahida
Format: Student Project
Language:English
Published: 2017
Subjects:
Online Access:https://ir.uitm.edu.my/id/eprint/35095/
_version_ 1848808705072562176
author Yasir, Norsyahida
author_facet Yasir, Norsyahida
author_sort Yasir, Norsyahida
building UiTM Institutional Repository
collection Online Access
description Piper betleleaves was extracted by soxhlet extraction method withhexane, chloroform and methanol solvent. The yield of crude extraction of hexane, chloroform and methanol is 9.53%, 13.47% and 7.86% respectively. All three types of crude extraction was undergo Thin Layer Chromatography (TLC) analysis to observed the presence of chemical constituent for each crude. Hexane crude was used dichloromethane;chloroform (3:2) as solvent system. Chloroform crude was used hexane;choroform with ratio 2:3 as a solvent system that produced several spot of chemical constituent of the TLC plate. Methanol crude was used solvent system of hexane;ethylacetate (4.5:0.5). All crude extracts was undergo microbial activity with gram positive bacteria Staphylococcus aureus, Bacillus subtilis and gram negative bacteriaEscherichia coliand Salmonella enterica. The crude has shown significant activity against bacteria Salmonella sp. with larger inhibition, followed by Bacillus subtilis, Staphylococcus aureus, and Escherichia coli. The crude also tested for antioxidant activity by DPPH Radical Scavenging method. The methanol crude extract gave highest percentage of inhibition of antioxidant rather than chloroform and hexane because the presence of phenolic compound.
first_indexed 2025-11-14T23:02:57Z
format Student Project
id uitm-35095
institution Universiti Teknologi MARA
institution_category Local University
language English
last_indexed 2025-11-14T23:02:57Z
publishDate 2017
recordtype eprints
repository_type Digital Repository
spelling uitm-350952020-10-15T15:01:29Z https://ir.uitm.edu.my/id/eprint/35095/ Antioxidant and antimicrobial activities of piper betle / Norsyahida Yasir Yasir, Norsyahida Laboratories. General works Extraction (Chemistry) Piper betleleaves was extracted by soxhlet extraction method withhexane, chloroform and methanol solvent. The yield of crude extraction of hexane, chloroform and methanol is 9.53%, 13.47% and 7.86% respectively. All three types of crude extraction was undergo Thin Layer Chromatography (TLC) analysis to observed the presence of chemical constituent for each crude. Hexane crude was used dichloromethane;chloroform (3:2) as solvent system. Chloroform crude was used hexane;choroform with ratio 2:3 as a solvent system that produced several spot of chemical constituent of the TLC plate. Methanol crude was used solvent system of hexane;ethylacetate (4.5:0.5). All crude extracts was undergo microbial activity with gram positive bacteria Staphylococcus aureus, Bacillus subtilis and gram negative bacteriaEscherichia coliand Salmonella enterica. The crude has shown significant activity against bacteria Salmonella sp. with larger inhibition, followed by Bacillus subtilis, Staphylococcus aureus, and Escherichia coli. The crude also tested for antioxidant activity by DPPH Radical Scavenging method. The methanol crude extract gave highest percentage of inhibition of antioxidant rather than chloroform and hexane because the presence of phenolic compound. 2017 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/35095/1/35095.pdf Yasir, Norsyahida (2017) Antioxidant and antimicrobial activities of piper betle / Norsyahida Yasir. (2017) [Student Project] (Unpublished)
spellingShingle Laboratories. General works
Extraction (Chemistry)
Yasir, Norsyahida
Antioxidant and antimicrobial activities of piper betle / Norsyahida Yasir
title Antioxidant and antimicrobial activities of piper betle / Norsyahida Yasir
title_full Antioxidant and antimicrobial activities of piper betle / Norsyahida Yasir
title_fullStr Antioxidant and antimicrobial activities of piper betle / Norsyahida Yasir
title_full_unstemmed Antioxidant and antimicrobial activities of piper betle / Norsyahida Yasir
title_short Antioxidant and antimicrobial activities of piper betle / Norsyahida Yasir
title_sort antioxidant and antimicrobial activities of piper betle / norsyahida yasir
topic Laboratories. General works
Extraction (Chemistry)
url https://ir.uitm.edu.my/id/eprint/35095/