Identification of pathogenic bacteria isolated from raw and after sand filtration water at Lubok Buntar Water Treatment Plant / Nur Hafizah Zakaria …[et al.]
This study focused on the identification of pathogenic bacteria in raw water intake and after sand filtration for drinking water treatment plant during flood event in 2014. The samples was collected from the Lubok Buntar Water Treatment Plant (WTP) and processed through bacterial isolation using cho...
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| Format: | Article |
| Language: | English |
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Universiti Teknologi MARA Shah Alam
2017
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| Online Access: | https://ir.uitm.edu.my/id/eprint/34790/ |
| _version_ | 1848808631362912256 |
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| author | Zakaria, Nur Hafizah Tajarudin, Husnul Azan Mohd Sapingi, Mohd Sharizal Murshed, Mohamad Fared |
| author_facet | Zakaria, Nur Hafizah Tajarudin, Husnul Azan Mohd Sapingi, Mohd Sharizal Murshed, Mohamad Fared |
| author_sort | Zakaria, Nur Hafizah |
| building | UiTM Institutional Repository |
| collection | Online Access |
| description | This study focused on the identification of pathogenic bacteria in raw water intake and after sand filtration for drinking water treatment plant during flood event in 2014. The samples was collected from the Lubok Buntar Water Treatment Plant (WTP) and processed through bacterial isolation using chocolate agar as a media. The isolation process conducted based on serial samples dilution and streaking method prior to DNA extraction. Deoxyribonucleic acid (DNA) extraction kit was used to get selected bacteria DNA and further analysis using Polymerase Chain Reaction (PCR) test and electrophoresis to get DNA sequences. The Basic Local Alignment Search Tool (BLAST) analysis was employed to identify the species of the isolated bacteria. As a result, Pantoeaagglomerans and Enterobacter sp. were found in raw and filtered water sample and indicating the same family types. It was concluded that bacteria of the same species were found before and after sand filtration and need to be removed by disinfectant process. The findings also indicated that all the physicochemical parameters measured were within the values prescribed by the Interim National Water Quality Standard (INWQS). |
| first_indexed | 2025-11-14T23:01:47Z |
| format | Article |
| id | uitm-34790 |
| institution | Universiti Teknologi MARA |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T23:01:47Z |
| publishDate | 2017 |
| publisher | Universiti Teknologi MARA Shah Alam |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uitm-347902020-09-29T03:39:26Z https://ir.uitm.edu.my/id/eprint/34790/ Identification of pathogenic bacteria isolated from raw and after sand filtration water at Lubok Buntar Water Treatment Plant / Nur Hafizah Zakaria …[et al.] srj Zakaria, Nur Hafizah Tajarudin, Husnul Azan Mohd Sapingi, Mohd Sharizal Murshed, Mohamad Fared Microbial ecology Bacteria This study focused on the identification of pathogenic bacteria in raw water intake and after sand filtration for drinking water treatment plant during flood event in 2014. The samples was collected from the Lubok Buntar Water Treatment Plant (WTP) and processed through bacterial isolation using chocolate agar as a media. The isolation process conducted based on serial samples dilution and streaking method prior to DNA extraction. Deoxyribonucleic acid (DNA) extraction kit was used to get selected bacteria DNA and further analysis using Polymerase Chain Reaction (PCR) test and electrophoresis to get DNA sequences. The Basic Local Alignment Search Tool (BLAST) analysis was employed to identify the species of the isolated bacteria. As a result, Pantoeaagglomerans and Enterobacter sp. were found in raw and filtered water sample and indicating the same family types. It was concluded that bacteria of the same species were found before and after sand filtration and need to be removed by disinfectant process. The findings also indicated that all the physicochemical parameters measured were within the values prescribed by the Interim National Water Quality Standard (INWQS). Universiti Teknologi MARA Shah Alam 2017-06 Article PeerReviewed text en https://ir.uitm.edu.my/id/eprint/34790/1/34790.pdf Zakaria, Nur Hafizah and Tajarudin, Husnul Azan and Mohd Sapingi, Mohd Sharizal and Murshed, Mohamad Fared (2017) Identification of pathogenic bacteria isolated from raw and after sand filtration water at Lubok Buntar Water Treatment Plant / Nur Hafizah Zakaria …[et al.]. (2017) Scientific Research Journal <https://ir.uitm.edu.my/view/publication/Scientific_Research_Journal.html>, 14 (1). pp. 41-52. ISSN 2289-649X https://srj.uitm.edu.my/ https://doi.org/10.24191/srj.v14i1.9371 https://doi.org/10.24191/srj.v14i1.9371 |
| spellingShingle | Microbial ecology Bacteria Zakaria, Nur Hafizah Tajarudin, Husnul Azan Mohd Sapingi, Mohd Sharizal Murshed, Mohamad Fared Identification of pathogenic bacteria isolated from raw and after sand filtration water at Lubok Buntar Water Treatment Plant / Nur Hafizah Zakaria …[et al.] |
| title | Identification of pathogenic bacteria isolated from raw and after sand filtration water at Lubok Buntar Water Treatment Plant / Nur Hafizah Zakaria …[et al.] |
| title_full | Identification of pathogenic bacteria isolated from raw and after sand filtration water at Lubok Buntar Water Treatment Plant / Nur Hafizah Zakaria …[et al.] |
| title_fullStr | Identification of pathogenic bacteria isolated from raw and after sand filtration water at Lubok Buntar Water Treatment Plant / Nur Hafizah Zakaria …[et al.] |
| title_full_unstemmed | Identification of pathogenic bacteria isolated from raw and after sand filtration water at Lubok Buntar Water Treatment Plant / Nur Hafizah Zakaria …[et al.] |
| title_short | Identification of pathogenic bacteria isolated from raw and after sand filtration water at Lubok Buntar Water Treatment Plant / Nur Hafizah Zakaria …[et al.] |
| title_sort | identification of pathogenic bacteria isolated from raw and after sand filtration water at lubok buntar water treatment plant / nur hafizah zakaria …[et al.] |
| topic | Microbial ecology Bacteria |
| url | https://ir.uitm.edu.my/id/eprint/34790/ https://ir.uitm.edu.my/id/eprint/34790/ https://ir.uitm.edu.my/id/eprint/34790/ |