Respond of seed culture of Ricinus communis toward different hormone treatment in vitro / Muhamad Saifulnizam Tahirudin
This research work aimed to highlight on the effect of various plant growth regulators namely 1-Naphthaleneacetic Acid (NAA) and 6-Benzylaminopurine (BAP) on inducing response toward Ricinus communis in vitro tissue culture. The response observe may be shoot formation, root formation or callus forma...
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| Format: | Student Project |
| Language: | English |
| Published: |
2016
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| Subjects: | |
| Online Access: | https://ir.uitm.edu.my/id/eprint/34502/ |
| _version_ | 1848808568362369024 |
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| author | Tahirudin, Muhamad Saifulnizam |
| author_facet | Tahirudin, Muhamad Saifulnizam |
| author_sort | Tahirudin, Muhamad Saifulnizam |
| building | UiTM Institutional Repository |
| collection | Online Access |
| description | This research work aimed to highlight on the effect of various plant growth regulators namely 1-Naphthaleneacetic Acid (NAA) and 6-Benzylaminopurine (BAP) on inducing response toward Ricinus communis in vitro tissue culture. The response observe may be shoot formation, root formation or callus formation that were initiated from seed explant of wild Ricinus communis plant located around Parit Tinggi, Kuala Pilah. Different combinations of hormone treatment were added into MS medium containing sucrose, macronutrient and micronutrient.
Combination of auxin, NAA and cytokinin, BAP gave different response toward the seed explant. After two weeks of inoculation in sterile environment, the response was observed from the seed explant. About 25% of the incubated seed germinated in the MS basal medium. As a result, 0.5, 1.5 and 2.0 mg/1 of NAA combined with 2.5mg/l BAP gave response where callus formation can be observed in this hormone treatment. Shoot formation was observed at 1.5 mg/I NAA and 2.5 BAP and at 1.0, 1.5, 2.0 and 2.5 mg/I NAA combined with 2.0 mg/1 BAP, there was an enlargement of seed explant where the seed enlarged from average of 0.5 cm to 2 cm. In conclusion, different combination of auxin and cytokinin give rise to different response toward the seed explant of Ricinus communis. |
| first_indexed | 2025-11-14T23:00:47Z |
| format | Student Project |
| id | uitm-34502 |
| institution | Universiti Teknologi MARA |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T23:00:47Z |
| publishDate | 2016 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uitm-345022020-09-28T04:11:50Z https://ir.uitm.edu.my/id/eprint/34502/ Respond of seed culture of Ricinus communis toward different hormone treatment in vitro / Muhamad Saifulnizam Tahirudin Tahirudin, Muhamad Saifulnizam Laboratories. General works Growth Growth. Development. Including pattern formation This research work aimed to highlight on the effect of various plant growth regulators namely 1-Naphthaleneacetic Acid (NAA) and 6-Benzylaminopurine (BAP) on inducing response toward Ricinus communis in vitro tissue culture. The response observe may be shoot formation, root formation or callus formation that were initiated from seed explant of wild Ricinus communis plant located around Parit Tinggi, Kuala Pilah. Different combinations of hormone treatment were added into MS medium containing sucrose, macronutrient and micronutrient. Combination of auxin, NAA and cytokinin, BAP gave different response toward the seed explant. After two weeks of inoculation in sterile environment, the response was observed from the seed explant. About 25% of the incubated seed germinated in the MS basal medium. As a result, 0.5, 1.5 and 2.0 mg/1 of NAA combined with 2.5mg/l BAP gave response where callus formation can be observed in this hormone treatment. Shoot formation was observed at 1.5 mg/I NAA and 2.5 BAP and at 1.0, 1.5, 2.0 and 2.5 mg/I NAA combined with 2.0 mg/1 BAP, there was an enlargement of seed explant where the seed enlarged from average of 0.5 cm to 2 cm. In conclusion, different combination of auxin and cytokinin give rise to different response toward the seed explant of Ricinus communis. 2016 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/34502/1/34502.pdf Tahirudin, Muhamad Saifulnizam (2016) Respond of seed culture of Ricinus communis toward different hormone treatment in vitro / Muhamad Saifulnizam Tahirudin. (2016) [Student Project] (Unpublished) |
| spellingShingle | Laboratories. General works Growth Growth. Development. Including pattern formation Tahirudin, Muhamad Saifulnizam Respond of seed culture of Ricinus communis toward different hormone treatment in vitro / Muhamad Saifulnizam Tahirudin |
| title | Respond of seed culture of Ricinus communis toward different hormone treatment in vitro / Muhamad Saifulnizam Tahirudin |
| title_full | Respond of seed culture of Ricinus communis toward different hormone treatment in vitro / Muhamad Saifulnizam Tahirudin |
| title_fullStr | Respond of seed culture of Ricinus communis toward different hormone treatment in vitro / Muhamad Saifulnizam Tahirudin |
| title_full_unstemmed | Respond of seed culture of Ricinus communis toward different hormone treatment in vitro / Muhamad Saifulnizam Tahirudin |
| title_short | Respond of seed culture of Ricinus communis toward different hormone treatment in vitro / Muhamad Saifulnizam Tahirudin |
| title_sort | respond of seed culture of ricinus communis toward different hormone treatment in vitro / muhamad saifulnizam tahirudin |
| topic | Laboratories. General works Growth Growth. Development. Including pattern formation |
| url | https://ir.uitm.edu.my/id/eprint/34502/ |