Screening of bacterial contamination on shopping trolley handles from supermarkets in Kuala Pilah / Saidatul Ashikin Mohd Asri

Shopping trolleys are used by the public in every supermarket worldwide. It is used by people from various backgrounds, especially in terms of jobs and personal hygiene practice which may lead to the transferring of bacteria from users’ hand to the handle or the other way round. The aims of this stu...

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Main Author: Mohd Asri, Saidatul Ashikin
Format: Student Project
Language:English
Published: 2018
Subjects:
Online Access:https://ir.uitm.edu.my/id/eprint/34353/
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author Mohd Asri, Saidatul Ashikin
author_facet Mohd Asri, Saidatul Ashikin
author_sort Mohd Asri, Saidatul Ashikin
building UiTM Institutional Repository
collection Online Access
description Shopping trolleys are used by the public in every supermarket worldwide. It is used by people from various backgrounds, especially in terms of jobs and personal hygiene practice which may lead to the transferring of bacteria from users’ hand to the handle or the other way round. The aims of this study were to isolate and screen Escherichia coli, Staphylococcus aureus and Salmonella spp. on shopping trolley handles from two different supermarkets in Kuala Pilah. Samples were cultivated on selective media and further characterization was done through Gram staining and biochemical tests such as Indole, methyl-red, Voges-Proskauer and citrate test. The results of this study showed that none of the three aforementioned bacteria were isolated, and the isolates from samples taken from the supermarkets harbored Citrobacter sp. and two unidentified bacteria, of Gram-positive and Gram-negative bacteria. The detection of Citrobacter sp. and possible colonization of S. aureus suggest that there are contamination of disease-causing bacteria on shopping trolley handles that calls for good hygiene practice.
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publishDate 2018
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spelling uitm-343532020-09-08T08:47:31Z https://ir.uitm.edu.my/id/eprint/34353/ Screening of bacterial contamination on shopping trolley handles from supermarkets in Kuala Pilah / Saidatul Ashikin Mohd Asri Mohd Asri, Saidatul Ashikin Laboratories. General works Bacteria Shopping trolleys are used by the public in every supermarket worldwide. It is used by people from various backgrounds, especially in terms of jobs and personal hygiene practice which may lead to the transferring of bacteria from users’ hand to the handle or the other way round. The aims of this study were to isolate and screen Escherichia coli, Staphylococcus aureus and Salmonella spp. on shopping trolley handles from two different supermarkets in Kuala Pilah. Samples were cultivated on selective media and further characterization was done through Gram staining and biochemical tests such as Indole, methyl-red, Voges-Proskauer and citrate test. The results of this study showed that none of the three aforementioned bacteria were isolated, and the isolates from samples taken from the supermarkets harbored Citrobacter sp. and two unidentified bacteria, of Gram-positive and Gram-negative bacteria. The detection of Citrobacter sp. and possible colonization of S. aureus suggest that there are contamination of disease-causing bacteria on shopping trolley handles that calls for good hygiene practice. 2018 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/34353/1/34353.pdf Mohd Asri, Saidatul Ashikin (2018) Screening of bacterial contamination on shopping trolley handles from supermarkets in Kuala Pilah / Saidatul Ashikin Mohd Asri. (2018) [Student Project] (Unpublished)
spellingShingle Laboratories. General works
Bacteria
Mohd Asri, Saidatul Ashikin
Screening of bacterial contamination on shopping trolley handles from supermarkets in Kuala Pilah / Saidatul Ashikin Mohd Asri
title Screening of bacterial contamination on shopping trolley handles from supermarkets in Kuala Pilah / Saidatul Ashikin Mohd Asri
title_full Screening of bacterial contamination on shopping trolley handles from supermarkets in Kuala Pilah / Saidatul Ashikin Mohd Asri
title_fullStr Screening of bacterial contamination on shopping trolley handles from supermarkets in Kuala Pilah / Saidatul Ashikin Mohd Asri
title_full_unstemmed Screening of bacterial contamination on shopping trolley handles from supermarkets in Kuala Pilah / Saidatul Ashikin Mohd Asri
title_short Screening of bacterial contamination on shopping trolley handles from supermarkets in Kuala Pilah / Saidatul Ashikin Mohd Asri
title_sort screening of bacterial contamination on shopping trolley handles from supermarkets in kuala pilah / saidatul ashikin mohd asri
topic Laboratories. General works
Bacteria
url https://ir.uitm.edu.my/id/eprint/34353/