Isolation and characterization of gram negative bacteria that may cause UTI from student toilets in UiTM Kuala Pilah / Nooradila Anzeli

In general, most of gram negative bacteria known as pathogenic and may spread due to the lack hygiene of the toilet. This problem can develop into serious illness like urinary tract infection (UTI). By using a sterile cotton swab, swabbing method was performed to swab different part of the toilet su...

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Main Author: Anzeli, Nooradila
Format: Student Project
Language:English
Published: 2017
Subjects:
Online Access:https://ir.uitm.edu.my/id/eprint/33528/
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author Anzeli, Nooradila
author_facet Anzeli, Nooradila
author_sort Anzeli, Nooradila
building UiTM Institutional Repository
collection Online Access
description In general, most of gram negative bacteria known as pathogenic and may spread due to the lack hygiene of the toilet. This problem can develop into serious illness like urinary tract infection (UTI). By using a sterile cotton swab, swabbing method was performed to swab different part of the toilet surface include of bowl, floor and seat. Samples were inoculated first on Nutrient agar before re-inoculated on MacConkey and Blood agar. All the samples were incubated at 37oC for 24 hours. Biochemical tests such as catalase, oxidase, IMViC and TSI proceed for bacterial identification. Results showed gram negative bacteria which are E.coli, Pseudomonas spp. and Klebsiella spp. were isolated from the students’ toilets. The result of this study indicates high presence of gram negative bacteria on students’ toilet in UiTM Kuala Pilah.
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institution Universiti Teknologi MARA
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publishDate 2017
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spelling uitm-335282020-08-14T02:40:15Z https://ir.uitm.edu.my/id/eprint/33528/ Isolation and characterization of gram negative bacteria that may cause UTI from student toilets in UiTM Kuala Pilah / Nooradila Anzeli Anzeli, Nooradila Laboratories. General works Bacteria In general, most of gram negative bacteria known as pathogenic and may spread due to the lack hygiene of the toilet. This problem can develop into serious illness like urinary tract infection (UTI). By using a sterile cotton swab, swabbing method was performed to swab different part of the toilet surface include of bowl, floor and seat. Samples were inoculated first on Nutrient agar before re-inoculated on MacConkey and Blood agar. All the samples were incubated at 37oC for 24 hours. Biochemical tests such as catalase, oxidase, IMViC and TSI proceed for bacterial identification. Results showed gram negative bacteria which are E.coli, Pseudomonas spp. and Klebsiella spp. were isolated from the students’ toilets. The result of this study indicates high presence of gram negative bacteria on students’ toilet in UiTM Kuala Pilah. 2017 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/33528/1/33528.pdf Anzeli, Nooradila (2017) Isolation and characterization of gram negative bacteria that may cause UTI from student toilets in UiTM Kuala Pilah / Nooradila Anzeli. (2017) [Student Project] (Unpublished)
spellingShingle Laboratories. General works
Bacteria
Anzeli, Nooradila
Isolation and characterization of gram negative bacteria that may cause UTI from student toilets in UiTM Kuala Pilah / Nooradila Anzeli
title Isolation and characterization of gram negative bacteria that may cause UTI from student toilets in UiTM Kuala Pilah / Nooradila Anzeli
title_full Isolation and characterization of gram negative bacteria that may cause UTI from student toilets in UiTM Kuala Pilah / Nooradila Anzeli
title_fullStr Isolation and characterization of gram negative bacteria that may cause UTI from student toilets in UiTM Kuala Pilah / Nooradila Anzeli
title_full_unstemmed Isolation and characterization of gram negative bacteria that may cause UTI from student toilets in UiTM Kuala Pilah / Nooradila Anzeli
title_short Isolation and characterization of gram negative bacteria that may cause UTI from student toilets in UiTM Kuala Pilah / Nooradila Anzeli
title_sort isolation and characterization of gram negative bacteria that may cause uti from student toilets in uitm kuala pilah / nooradila anzeli
topic Laboratories. General works
Bacteria
url https://ir.uitm.edu.my/id/eprint/33528/