Isolation and characterization of bacteria isolated from decayed fruits / Intan Munirah Zulkahar

Malaysia paradies for local fruits such as banana (Musa paradisiaca), papaya (Carica papaya L.) and pineapple ( Ananas comosus L.). Bacteria easily spoiled the local fruits which caused change in the appearance, colour and smell of the fruits. Therefore, this study were aim to isolate and identify p...

Full description

Bibliographic Details
Main Author: Zulkahar, Intan Munirah
Format: Student Project
Language:English
Published: 2017
Subjects:
Online Access:https://ir.uitm.edu.my/id/eprint/32635/
_version_ 1848808075235950592
author Zulkahar, Intan Munirah
author_facet Zulkahar, Intan Munirah
author_sort Zulkahar, Intan Munirah
building UiTM Institutional Repository
collection Online Access
description Malaysia paradies for local fruits such as banana (Musa paradisiaca), papaya (Carica papaya L.) and pineapple ( Ananas comosus L.). Bacteria easily spoiled the local fruits which caused change in the appearance, colour and smell of the fruits. Therefore, this study were aim to isolate and identify pathogenic bacteria by using morphological and molecular methods. Decayed fruits such as banana, papaya and pineapple samples were obtained from fruits shop at Kuala Pilah Negeri Sembilan district. In this study, six bacteria strain were successfully isolated known as P1, P2, B1, B2, N1 and N2 bacteria. Gram strain results demonstrated that one bacteria, B1 give a positive result while others five bacteria which were P1, P2, B2, N1 and N2 showed negative results with rod-shaped appearance. In addition, P1, P2, B1, B2, N1 and N2 bacteria colonies observation showed circular, entire, raised, smooth and shinning however it differed in term of size and optical characteristics. In addition, biochemical tests showed P1 and P2 bacteria belong to Klebsiella sp. and Escherichia sp. meanwhile others bacteria failed to give positive results. However, molecular analysis showed a negative results for P1 and P2 bacteria. Therefore in this study, bacteria samples from decayed fruits belong to Klebsiella sp. and Escherichia sp. Molecular analysis was recommended to comfirm the bacteria species.
first_indexed 2025-11-14T22:52:57Z
format Student Project
id uitm-32635
institution Universiti Teknologi MARA
institution_category Local University
language English
last_indexed 2025-11-14T22:52:57Z
publishDate 2017
recordtype eprints
repository_type Digital Repository
spelling uitm-326352020-07-17T06:54:55Z https://ir.uitm.edu.my/id/eprint/32635/ Isolation and characterization of bacteria isolated from decayed fruits / Intan Munirah Zulkahar Zulkahar, Intan Munirah Laboratories. General works Extraction (Chemistry) Bacteria Malaysia paradies for local fruits such as banana (Musa paradisiaca), papaya (Carica papaya L.) and pineapple ( Ananas comosus L.). Bacteria easily spoiled the local fruits which caused change in the appearance, colour and smell of the fruits. Therefore, this study were aim to isolate and identify pathogenic bacteria by using morphological and molecular methods. Decayed fruits such as banana, papaya and pineapple samples were obtained from fruits shop at Kuala Pilah Negeri Sembilan district. In this study, six bacteria strain were successfully isolated known as P1, P2, B1, B2, N1 and N2 bacteria. Gram strain results demonstrated that one bacteria, B1 give a positive result while others five bacteria which were P1, P2, B2, N1 and N2 showed negative results with rod-shaped appearance. In addition, P1, P2, B1, B2, N1 and N2 bacteria colonies observation showed circular, entire, raised, smooth and shinning however it differed in term of size and optical characteristics. In addition, biochemical tests showed P1 and P2 bacteria belong to Klebsiella sp. and Escherichia sp. meanwhile others bacteria failed to give positive results. However, molecular analysis showed a negative results for P1 and P2 bacteria. Therefore in this study, bacteria samples from decayed fruits belong to Klebsiella sp. and Escherichia sp. Molecular analysis was recommended to comfirm the bacteria species. 2017 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/32635/1/PPB_INTAN%20MUNIRAH%20ZULKAHAR%20AS%20N%2017_5.pdf Zulkahar, Intan Munirah (2017) Isolation and characterization of bacteria isolated from decayed fruits / Intan Munirah Zulkahar. (2017) [Student Project] (Unpublished)
spellingShingle Laboratories. General works
Extraction (Chemistry)
Bacteria
Zulkahar, Intan Munirah
Isolation and characterization of bacteria isolated from decayed fruits / Intan Munirah Zulkahar
title Isolation and characterization of bacteria isolated from decayed fruits / Intan Munirah Zulkahar
title_full Isolation and characterization of bacteria isolated from decayed fruits / Intan Munirah Zulkahar
title_fullStr Isolation and characterization of bacteria isolated from decayed fruits / Intan Munirah Zulkahar
title_full_unstemmed Isolation and characterization of bacteria isolated from decayed fruits / Intan Munirah Zulkahar
title_short Isolation and characterization of bacteria isolated from decayed fruits / Intan Munirah Zulkahar
title_sort isolation and characterization of bacteria isolated from decayed fruits / intan munirah zulkahar
topic Laboratories. General works
Extraction (Chemistry)
Bacteria
url https://ir.uitm.edu.my/id/eprint/32635/