Isolation and tentative identification of antioxidative constituents from dichloromethane extract of muraya koenigii leaves using chromatographic technique / Norhakimy Abdul Majid and Aiza Harun

This investigation aimed to identify antioxidative constituents from M. koeginii leaves using thin layer chromatographic (TLC) technique. The screening of phytochemicals was conducted using methanol extracts as well as the developed TLC of extracts. Isolation of antioxidative constituents was held u...

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Main Authors: Abdul Majid, Norhakimy, Harun, Aiza
Format: Article
Language:English
Published: Universiti Teknologi MARA Cawangan Pahang 2019
Subjects:
Online Access:https://ir.uitm.edu.my/id/eprint/31237/
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author Abdul Majid, Norhakimy
Harun, Aiza
author_facet Abdul Majid, Norhakimy
Harun, Aiza
author_sort Abdul Majid, Norhakimy
building UiTM Institutional Repository
collection Online Access
description This investigation aimed to identify antioxidative constituents from M. koeginii leaves using thin layer chromatographic (TLC) technique. The screening of phytochemicals was conducted using methanol extracts as well as the developed TLC of extracts. Isolation of antioxidative constituents was held using preparative TLC. Secondary metabolites that had been screened from methanol extract were recognized as tannins, alkaloids, flavanoid and terpenoids. Screening for phytochemical using developed TLC of extracts revealed the presence of antioxidative constituents from dichloromethane extract after spraying with DPPH reagent. The antioxidative constituents are proposed as (A) Cyclic Terpene ester,(B) 4-benzyloxy-4- [2,2,-dimethyl-4-dioxolanyl] butylaldehyde, (C) Oleic acid, (D) Piperidione (alkaloid derivatives) and (E) 9-oxo-methyl ester after analyzing with GCMS aided with Wiley Database matching individually. Based on its potent antioxidant properties, the use of M. koenigii leaves in the development of natural medicine for the treatment of various diseases cannot be neglected.
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spelling uitm-312372022-04-04T01:38:33Z https://ir.uitm.edu.my/id/eprint/31237/ Isolation and tentative identification of antioxidative constituents from dichloromethane extract of muraya koenigii leaves using chromatographic technique / Norhakimy Abdul Majid and Aiza Harun gadingst Abdul Majid, Norhakimy Harun, Aiza QD Chemistry Physical and theoretical chemistry TP Chemical technology This investigation aimed to identify antioxidative constituents from M. koeginii leaves using thin layer chromatographic (TLC) technique. The screening of phytochemicals was conducted using methanol extracts as well as the developed TLC of extracts. Isolation of antioxidative constituents was held using preparative TLC. Secondary metabolites that had been screened from methanol extract were recognized as tannins, alkaloids, flavanoid and terpenoids. Screening for phytochemical using developed TLC of extracts revealed the presence of antioxidative constituents from dichloromethane extract after spraying with DPPH reagent. The antioxidative constituents are proposed as (A) Cyclic Terpene ester,(B) 4-benzyloxy-4- [2,2,-dimethyl-4-dioxolanyl] butylaldehyde, (C) Oleic acid, (D) Piperidione (alkaloid derivatives) and (E) 9-oxo-methyl ester after analyzing with GCMS aided with Wiley Database matching individually. Based on its potent antioxidant properties, the use of M. koenigii leaves in the development of natural medicine for the treatment of various diseases cannot be neglected. Universiti Teknologi MARA Cawangan Pahang 2019 Article PeerReviewed text en https://ir.uitm.edu.my/id/eprint/31237/1/31237.pdf Abdul Majid, Norhakimy and Harun, Aiza (2019) Isolation and tentative identification of antioxidative constituents from dichloromethane extract of muraya koenigii leaves using chromatographic technique / Norhakimy Abdul Majid and Aiza Harun. (2019) Gading Journal for Science and Technology <https://ir.uitm.edu.my/view/publication/Gading_Journal_for_Science_and_Technology.html>, 2 (1). pp. 31-37. ISSN 2637-0018
spellingShingle QD Chemistry
Physical and theoretical chemistry
TP Chemical technology
Abdul Majid, Norhakimy
Harun, Aiza
Isolation and tentative identification of antioxidative constituents from dichloromethane extract of muraya koenigii leaves using chromatographic technique / Norhakimy Abdul Majid and Aiza Harun
title Isolation and tentative identification of antioxidative constituents from dichloromethane extract of muraya koenigii leaves using chromatographic technique / Norhakimy Abdul Majid and Aiza Harun
title_full Isolation and tentative identification of antioxidative constituents from dichloromethane extract of muraya koenigii leaves using chromatographic technique / Norhakimy Abdul Majid and Aiza Harun
title_fullStr Isolation and tentative identification of antioxidative constituents from dichloromethane extract of muraya koenigii leaves using chromatographic technique / Norhakimy Abdul Majid and Aiza Harun
title_full_unstemmed Isolation and tentative identification of antioxidative constituents from dichloromethane extract of muraya koenigii leaves using chromatographic technique / Norhakimy Abdul Majid and Aiza Harun
title_short Isolation and tentative identification of antioxidative constituents from dichloromethane extract of muraya koenigii leaves using chromatographic technique / Norhakimy Abdul Majid and Aiza Harun
title_sort isolation and tentative identification of antioxidative constituents from dichloromethane extract of muraya koenigii leaves using chromatographic technique / norhakimy abdul majid and aiza harun
topic QD Chemistry
Physical and theoretical chemistry
TP Chemical technology
url https://ir.uitm.edu.my/id/eprint/31237/