Antioxidative constituents from twig and leaves of jasminum sambac / Nurul Fatin Fakhira Mohd Padli ... [et. al.]

This study aimed to investigate new antioxidative constituents from twig and leaves of Jasminium sambac (Oleaceae family) as potent antioxidant sources. The antioxidant behaviour of each extract from twig and leaves of J. sambac was assessed qualitatively by spraying the thin layer chrom...

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Main Authors: Mohd Padli, Nurul Fatin Fakhira, Sulaiman, Siti Nazira, Harun, Aiza, Daud, Shaari, Harith, Siti Suhaila, Abdul Aziz, Noorshilawati
Format: Article
Language:English
Published: Universiti Teknologi MARA Cawangan Pahang 2019
Subjects:
Online Access:https://ir.uitm.edu.my/id/eprint/31174/
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author Mohd Padli, Nurul Fatin Fakhira
Sulaiman, Siti Nazira
Harun, Aiza
Daud, Shaari
Harith, Siti Suhaila
Abdul Aziz, Noorshilawati
author_facet Mohd Padli, Nurul Fatin Fakhira
Sulaiman, Siti Nazira
Harun, Aiza
Daud, Shaari
Harith, Siti Suhaila
Abdul Aziz, Noorshilawati
author_sort Mohd Padli, Nurul Fatin Fakhira
building UiTM Institutional Repository
collection Online Access
description This study aimed to investigate new antioxidative constituents from twig and leaves of Jasminium sambac (Oleaceae family) as potent antioxidant sources. The antioxidant behaviour of each extract from twig and leaves of J. sambac was assessed qualitatively by spraying the thin layer chromatography (TLC) of extracts with 2,2-diphenyl-1-picrylhydrazyl (DPPH) and quantitatively by DPPH radical scavenging method. The collection of antioxidative constituents was carried out using centrifugal chromatography. The chemical structures of all constituents were identified from Gas Chromatography Mass Spectrometer (GCMS). The qualitative Thin Layer Chromatography (TLC) screening of antioxidants showed that all types of extracts from J. sambac leaves demonstrated moderate antioxidant behaviour as well as the extracts from twig parts. The quantitative DPPH radical scavenging activity of leaves and twig extracts revealed dichloromethane (DCM) extract scavenged DPPH free radicals more effective at IC50of 6.24 ppm and 5.22 ppm respectively compared to petroleum ether extract and methanol extract where both extracts exhibited IC50more than 100 ppm. The antioxidative constituents isolated from DCM extract of twig of J. sambac were recognized as isoamyl nitrite and benzophenone whereby benzophenone was also found in DCM extract of J.sambac leaves. Thus, J. sambaccan be considered as a promising source of bioactive compounds with potent antioxidant properties which can be utilized as baseline information to overcome health disorders.
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spelling uitm-311742020-12-09T07:35:18Z https://ir.uitm.edu.my/id/eprint/31174/ Antioxidative constituents from twig and leaves of jasminum sambac / Nurul Fatin Fakhira Mohd Padli ... [et. al.] gadingst Mohd Padli, Nurul Fatin Fakhira Sulaiman, Siti Nazira Harun, Aiza Daud, Shaari Harith, Siti Suhaila Abdul Aziz, Noorshilawati SB Plant culture Plant biotechnology This study aimed to investigate new antioxidative constituents from twig and leaves of Jasminium sambac (Oleaceae family) as potent antioxidant sources. The antioxidant behaviour of each extract from twig and leaves of J. sambac was assessed qualitatively by spraying the thin layer chromatography (TLC) of extracts with 2,2-diphenyl-1-picrylhydrazyl (DPPH) and quantitatively by DPPH radical scavenging method. The collection of antioxidative constituents was carried out using centrifugal chromatography. The chemical structures of all constituents were identified from Gas Chromatography Mass Spectrometer (GCMS). The qualitative Thin Layer Chromatography (TLC) screening of antioxidants showed that all types of extracts from J. sambac leaves demonstrated moderate antioxidant behaviour as well as the extracts from twig parts. The quantitative DPPH radical scavenging activity of leaves and twig extracts revealed dichloromethane (DCM) extract scavenged DPPH free radicals more effective at IC50of 6.24 ppm and 5.22 ppm respectively compared to petroleum ether extract and methanol extract where both extracts exhibited IC50more than 100 ppm. The antioxidative constituents isolated from DCM extract of twig of J. sambac were recognized as isoamyl nitrite and benzophenone whereby benzophenone was also found in DCM extract of J.sambac leaves. Thus, J. sambaccan be considered as a promising source of bioactive compounds with potent antioxidant properties which can be utilized as baseline information to overcome health disorders. Universiti Teknologi MARA Cawangan Pahang 2019-09-30 Article PeerReviewed text en https://ir.uitm.edu.my/id/eprint/31174/1/31174.pdf Mohd Padli, Nurul Fatin Fakhira and Sulaiman, Siti Nazira and Harun, Aiza and Daud, Shaari and Harith, Siti Suhaila and Abdul Aziz, Noorshilawati (2019) Antioxidative constituents from twig and leaves of jasminum sambac / Nurul Fatin Fakhira Mohd Padli ... [et. al.]. (2019) Gading Journal for Science and Technology <https://ir.uitm.edu.my/view/publication/Gading_Journal_for_Science_and_Technology.html>, 2 (2). pp. 8-16. ISSN 2637-0018
spellingShingle SB Plant culture
Plant biotechnology
Mohd Padli, Nurul Fatin Fakhira
Sulaiman, Siti Nazira
Harun, Aiza
Daud, Shaari
Harith, Siti Suhaila
Abdul Aziz, Noorshilawati
Antioxidative constituents from twig and leaves of jasminum sambac / Nurul Fatin Fakhira Mohd Padli ... [et. al.]
title Antioxidative constituents from twig and leaves of jasminum sambac / Nurul Fatin Fakhira Mohd Padli ... [et. al.]
title_full Antioxidative constituents from twig and leaves of jasminum sambac / Nurul Fatin Fakhira Mohd Padli ... [et. al.]
title_fullStr Antioxidative constituents from twig and leaves of jasminum sambac / Nurul Fatin Fakhira Mohd Padli ... [et. al.]
title_full_unstemmed Antioxidative constituents from twig and leaves of jasminum sambac / Nurul Fatin Fakhira Mohd Padli ... [et. al.]
title_short Antioxidative constituents from twig and leaves of jasminum sambac / Nurul Fatin Fakhira Mohd Padli ... [et. al.]
title_sort antioxidative constituents from twig and leaves of jasminum sambac / nurul fatin fakhira mohd padli ... [et. al.]
topic SB Plant culture
Plant biotechnology
url https://ir.uitm.edu.my/id/eprint/31174/