Staphylococcus aureus nasal carriage in medical students of Universiti Teknologi MARA / Zaini Mohd Zain … [et al.]
To determine the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) nasal carriage and detection of S. aureus leukotoxins among medical students of Universiti Teknologi MARA. Methods: Both sides of the anterior nares of 136 volunteers, comprising 68 preclinical and 68 clinical medical...
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| Format: | Article |
| Language: | English |
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Universiti Teknologi MARA Cawangan Selangor
2017
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| Online Access: | https://ir.uitm.edu.my/id/eprint/30119/ |
| _version_ | 1848807416168185856 |
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| author | Mohd Zain, Zaini Johari, Muhammad Fikri Mohd Husin, Nurul Shahirah Rozman, Nurul Syamimi Ab Rashid, Athirah Bahanuddin, Latifah Mohamad Zain, Muhammad Ilyas Danial |
| author_facet | Mohd Zain, Zaini Johari, Muhammad Fikri Mohd Husin, Nurul Shahirah Rozman, Nurul Syamimi Ab Rashid, Athirah Bahanuddin, Latifah Mohamad Zain, Muhammad Ilyas Danial |
| author_sort | Mohd Zain, Zaini |
| building | UiTM Institutional Repository |
| collection | Online Access |
| description | To determine the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) nasal carriage and detection of S. aureus leukotoxins among medical students of Universiti Teknologi MARA. Methods: Both sides of the anterior nares of 136 volunteers, comprising 68 preclinical and 68 clinical medical students, were swabbed and immediately cultured onto mannitol salt agar for growth of S. aureus. Standard microbiological techniques were conducted to identify and confirm the S. aureus colonies and susceptibility test against oxacillin were conducted by using Kirby-Bauer method to determine their resistance to methicillin. Polymerase chain reaction was performed for detection of leukotoxins, i.e., Panton-Valentine Leukocidin (PVL) and -haemolysin genes. Results: Nineteen students (14%) consisting of 10 preclinical (14.7%) and 9 clinical (13.2%) were nasal carriers of S. aureus. However, none of the S. aureus isolates were MRSA. No PVL gene was detected but eight of them were positive for -haemolysin gene. Conclusion: There were no MRSA nasal carriers among the medical students, but a low prevalence of S. aureus nasal carriers was detected. These carriers do not pose as high risk because none of the strains of S. aureus possess both the -haemolysin toxin and the PVL toxin that are associated with tissue necrosis. |
| first_indexed | 2025-11-14T22:42:28Z |
| format | Article |
| id | uitm-30119 |
| institution | Universiti Teknologi MARA |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T22:42:28Z |
| publishDate | 2017 |
| publisher | Universiti Teknologi MARA Cawangan Selangor |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uitm-301192020-04-22T05:46:44Z https://ir.uitm.edu.my/id/eprint/30119/ Staphylococcus aureus nasal carriage in medical students of Universiti Teknologi MARA / Zaini Mohd Zain … [et al.] jchs Mohd Zain, Zaini Johari, Muhammad Fikri Mohd Husin, Nurul Shahirah Rozman, Nurul Syamimi Ab Rashid, Athirah Bahanuddin, Latifah Mohamad Zain, Muhammad Ilyas Danial QR Microbiology RC Internal Medicine Infectious and parasitic diseases To determine the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) nasal carriage and detection of S. aureus leukotoxins among medical students of Universiti Teknologi MARA. Methods: Both sides of the anterior nares of 136 volunteers, comprising 68 preclinical and 68 clinical medical students, were swabbed and immediately cultured onto mannitol salt agar for growth of S. aureus. Standard microbiological techniques were conducted to identify and confirm the S. aureus colonies and susceptibility test against oxacillin were conducted by using Kirby-Bauer method to determine their resistance to methicillin. Polymerase chain reaction was performed for detection of leukotoxins, i.e., Panton-Valentine Leukocidin (PVL) and -haemolysin genes. Results: Nineteen students (14%) consisting of 10 preclinical (14.7%) and 9 clinical (13.2%) were nasal carriers of S. aureus. However, none of the S. aureus isolates were MRSA. No PVL gene was detected but eight of them were positive for -haemolysin gene. Conclusion: There were no MRSA nasal carriers among the medical students, but a low prevalence of S. aureus nasal carriers was detected. These carriers do not pose as high risk because none of the strains of S. aureus possess both the -haemolysin toxin and the PVL toxin that are associated with tissue necrosis. Universiti Teknologi MARA Cawangan Selangor 2017-06 Article PeerReviewed text en https://ir.uitm.edu.my/id/eprint/30119/1/AJ_ZAINI%20MOHD%20ZAIN%20JCHS%20B%2017.pdf Mohd Zain, Zaini and Johari, Muhammad Fikri and Mohd Husin, Nurul Shahirah and Rozman, Nurul Syamimi and Ab Rashid, Athirah and Bahanuddin, Latifah and Mohamad Zain, Muhammad Ilyas Danial (2017) Staphylococcus aureus nasal carriage in medical students of Universiti Teknologi MARA / Zaini Mohd Zain … [et al.]. (2017) Journal of Clinical and Health Sciences <https://ir.uitm.edu.my/view/publication/Journal_of_Clinical_and_Health_Sciences.html>, 2 (1). pp. 1-6. ISSN 0127-984X https://jchs-medicine.uitm.edu.my/index.php |
| spellingShingle | QR Microbiology RC Internal Medicine Infectious and parasitic diseases Mohd Zain, Zaini Johari, Muhammad Fikri Mohd Husin, Nurul Shahirah Rozman, Nurul Syamimi Ab Rashid, Athirah Bahanuddin, Latifah Mohamad Zain, Muhammad Ilyas Danial Staphylococcus aureus nasal carriage in medical students of Universiti Teknologi MARA / Zaini Mohd Zain … [et al.] |
| title | Staphylococcus aureus nasal carriage in medical students of Universiti Teknologi MARA / Zaini Mohd Zain … [et al.] |
| title_full | Staphylococcus aureus nasal carriage in medical students of Universiti Teknologi MARA / Zaini Mohd Zain … [et al.] |
| title_fullStr | Staphylococcus aureus nasal carriage in medical students of Universiti Teknologi MARA / Zaini Mohd Zain … [et al.] |
| title_full_unstemmed | Staphylococcus aureus nasal carriage in medical students of Universiti Teknologi MARA / Zaini Mohd Zain … [et al.] |
| title_short | Staphylococcus aureus nasal carriage in medical students of Universiti Teknologi MARA / Zaini Mohd Zain … [et al.] |
| title_sort | staphylococcus aureus nasal carriage in medical students of universiti teknologi mara / zaini mohd zain … [et al.] |
| topic | QR Microbiology RC Internal Medicine Infectious and parasitic diseases |
| url | https://ir.uitm.edu.my/id/eprint/30119/ https://ir.uitm.edu.my/id/eprint/30119/ |