Identification and characterization of Enterocytozoon Bieneusi isolates from the proto—Malay and Senoi tribes in Sungai Lembing, Pahang

Enterocytozaon bieneusi is most common of microsporidian species that infects many types of cells including human and animals. These organisms are causing long lasting diarrhea primarily in immunocompromised patients but microsporidjosis is also higher in immunooompetent individuals. The previous st...

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Main Author: Azhari, Azah Ashikin
Format: Thesis
Language:English
Published: 2016
Online Access:https://ir.uitm.edu.my/id/eprint/27665/
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author Azhari, Azah Ashikin
author_facet Azhari, Azah Ashikin
author_sort Azhari, Azah Ashikin
building UiTM Institutional Repository
collection Online Access
description Enterocytozaon bieneusi is most common of microsporidian species that infects many types of cells including human and animals. These organisms are causing long lasting diarrhea primarily in immunocompromised patients but microsporidjosis is also higher in immunooompetent individuals. The previous study of microsporidial infection is mostly focused on immunodeficiency or immunosuppressive individuals. Information regarding microsporidial infections among immunocompetent individuals is lacking. Hence, this cross sectional study was carried out to determine prevalence of E‘ bieneusi infection and to find out the occurrence of E. bieneusi according to age group and gender among the Prom-Malay and Senoi tribes in Sungai Lembing, Pahang, Malaysia. Stool samples were collected fiom 209 voluntary participants; 96 males and 113 females that aged between one to 85 years old. Demographic data, socioeconomic, environmental, and behavioral infomation was collected by using pre-tested questionnaire. Overall prevalence of E‘ bieneusi infection was 3.83 % (8/209). From the observation, majority of individual infected with E. bieneusi was male (833 %) can be observed in those aged 215 years old‘ These samples were detected Small Subunit rDNA gene by using Polymerase chain reaction amplification with specific primer EBIEFl and EBIER], as demonstrated by an amplicon of 607-bp. This is the first report of E‘ bieneusi in Malaysia. It is hoped that these findings will allow greater emphasis from public health authrities through public education on personal hygiene and the consumption of boiled or filtered water in order to reduce the infection.
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spelling uitm-276652025-05-16T11:24:40Z https://ir.uitm.edu.my/id/eprint/27665/ Identification and characterization of Enterocytozoon Bieneusi isolates from the proto—Malay and Senoi tribes in Sungai Lembing, Pahang Azhari, Azah Ashikin Enterocytozaon bieneusi is most common of microsporidian species that infects many types of cells including human and animals. These organisms are causing long lasting diarrhea primarily in immunocompromised patients but microsporidjosis is also higher in immunooompetent individuals. The previous study of microsporidial infection is mostly focused on immunodeficiency or immunosuppressive individuals. Information regarding microsporidial infections among immunocompetent individuals is lacking. Hence, this cross sectional study was carried out to determine prevalence of E‘ bieneusi infection and to find out the occurrence of E. bieneusi according to age group and gender among the Prom-Malay and Senoi tribes in Sungai Lembing, Pahang, Malaysia. Stool samples were collected fiom 209 voluntary participants; 96 males and 113 females that aged between one to 85 years old. Demographic data, socioeconomic, environmental, and behavioral infomation was collected by using pre-tested questionnaire. Overall prevalence of E‘ bieneusi infection was 3.83 % (8/209). From the observation, majority of individual infected with E. bieneusi was male (833 %) can be observed in those aged 215 years old‘ These samples were detected Small Subunit rDNA gene by using Polymerase chain reaction amplification with specific primer EBIEFl and EBIER], as demonstrated by an amplicon of 607-bp. This is the first report of E‘ bieneusi in Malaysia. It is hoped that these findings will allow greater emphasis from public health authrities through public education on personal hygiene and the consumption of boiled or filtered water in order to reduce the infection. 2016 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/27665/1/TD_AZAH%20ASHIKIN%20AZHARI%20HS%2015_5.pdf Azhari, Azah Ashikin (2016) Identification and characterization of Enterocytozoon Bieneusi isolates from the proto—Malay and Senoi tribes in Sungai Lembing, Pahang. (2016) Degree thesis, thesis, Universiti Teknologi MARA. <http://terminalib.uitm.edu.my/27665.pdf>
spellingShingle Azhari, Azah Ashikin
Identification and characterization of Enterocytozoon Bieneusi isolates from the proto—Malay and Senoi tribes in Sungai Lembing, Pahang
title Identification and characterization of Enterocytozoon Bieneusi isolates from the proto—Malay and Senoi tribes in Sungai Lembing, Pahang
title_full Identification and characterization of Enterocytozoon Bieneusi isolates from the proto—Malay and Senoi tribes in Sungai Lembing, Pahang
title_fullStr Identification and characterization of Enterocytozoon Bieneusi isolates from the proto—Malay and Senoi tribes in Sungai Lembing, Pahang
title_full_unstemmed Identification and characterization of Enterocytozoon Bieneusi isolates from the proto—Malay and Senoi tribes in Sungai Lembing, Pahang
title_short Identification and characterization of Enterocytozoon Bieneusi isolates from the proto—Malay and Senoi tribes in Sungai Lembing, Pahang
title_sort identification and characterization of enterocytozoon bieneusi isolates from the proto—malay and senoi tribes in sungai lembing, pahang
url https://ir.uitm.edu.my/id/eprint/27665/