Detection of DEL phenotype in RhD negative blood by freeze-thaw elution technique and sequence-specific prime polymerase chain reaction / Nordiana Nordin
The weakest weak D called DEL phenotype was able to be detected via serological adsorption elution and molecular genotyping. In this study, the freeze-thaw elution and ssp-peR were used to detect the DEL phenotype. A total of 43 RhD negative samples collected from the National Blood Centre were sero...
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
2015
|
| Subjects: | |
| Online Access: | https://ir.uitm.edu.my/id/eprint/27365/ |
| _version_ | 1848806753965178880 |
|---|---|
| author | Nordin, Nordiana |
| author_facet | Nordin, Nordiana |
| author_sort | Nordin, Nordiana |
| building | UiTM Institutional Repository |
| collection | Online Access |
| description | The weakest weak D called DEL phenotype was able to be detected via serological adsorption elution and molecular genotyping. In this study, the freeze-thaw elution and ssp-peR were used to detect the DEL phenotype. A total of 43 RhD negative samples collected from the National Blood Centre were serologically tested for Rh phenotyping and screened for DEL phenotype by both serological freeze-thaw elution and SSP-PCR. Based on the Rh phenotyping, the 43 RhD negative samples consisted of 34 ccee phenotype, 6 Ccee phenotype, 2 ccEe phenotype and 1 CCee phenotype. No DEL phenotype was detected by serological freeze-thaw elution but 2.33% (1/43) from the samples were detected for DEL phenotype that carried the RHD1227A allele by SSP-PCR. As a conclusion, the aim of this study was accomplished by the ability to detect DEL phenotype via molecular genotyping technique in distinguishing the DEL phenotype from truly D negative but not for freeze thaw elution technique. |
| first_indexed | 2025-11-14T22:31:57Z |
| format | Thesis |
| id | uitm-27365 |
| institution | Universiti Teknologi MARA |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T22:31:57Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uitm-273652020-01-21T03:43:48Z https://ir.uitm.edu.my/id/eprint/27365/ Detection of DEL phenotype in RhD negative blood by freeze-thaw elution technique and sequence-specific prime polymerase chain reaction / Nordiana Nordin Nordin, Nordiana Clinical pathology. Laboratory technique Examination of the blood The weakest weak D called DEL phenotype was able to be detected via serological adsorption elution and molecular genotyping. In this study, the freeze-thaw elution and ssp-peR were used to detect the DEL phenotype. A total of 43 RhD negative samples collected from the National Blood Centre were serologically tested for Rh phenotyping and screened for DEL phenotype by both serological freeze-thaw elution and SSP-PCR. Based on the Rh phenotyping, the 43 RhD negative samples consisted of 34 ccee phenotype, 6 Ccee phenotype, 2 ccEe phenotype and 1 CCee phenotype. No DEL phenotype was detected by serological freeze-thaw elution but 2.33% (1/43) from the samples were detected for DEL phenotype that carried the RHD1227A allele by SSP-PCR. As a conclusion, the aim of this study was accomplished by the ability to detect DEL phenotype via molecular genotyping technique in distinguishing the DEL phenotype from truly D negative but not for freeze thaw elution technique. 2015 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/27365/1/TD_NORDIANA%20NORDIN%20HS%2015_5.pdf Nordin, Nordiana (2015) Detection of DEL phenotype in RhD negative blood by freeze-thaw elution technique and sequence-specific prime polymerase chain reaction / Nordiana Nordin. (2015) Degree thesis, thesis, Universiti Teknologi MARA. <http://terminalib.uitm.edu.my/27365.pdf> |
| spellingShingle | Clinical pathology. Laboratory technique Examination of the blood Nordin, Nordiana Detection of DEL phenotype in RhD negative blood by freeze-thaw elution technique and sequence-specific prime polymerase chain reaction / Nordiana Nordin |
| title | Detection of DEL phenotype in RhD negative blood by freeze-thaw elution technique and sequence-specific prime polymerase chain reaction / Nordiana Nordin |
| title_full | Detection of DEL phenotype in RhD negative blood by freeze-thaw elution technique and sequence-specific prime polymerase chain reaction / Nordiana Nordin |
| title_fullStr | Detection of DEL phenotype in RhD negative blood by freeze-thaw elution technique and sequence-specific prime polymerase chain reaction / Nordiana Nordin |
| title_full_unstemmed | Detection of DEL phenotype in RhD negative blood by freeze-thaw elution technique and sequence-specific prime polymerase chain reaction / Nordiana Nordin |
| title_short | Detection of DEL phenotype in RhD negative blood by freeze-thaw elution technique and sequence-specific prime polymerase chain reaction / Nordiana Nordin |
| title_sort | detection of del phenotype in rhd negative blood by freeze-thaw elution technique and sequence-specific prime polymerase chain reaction / nordiana nordin |
| topic | Clinical pathology. Laboratory technique Examination of the blood |
| url | https://ir.uitm.edu.my/id/eprint/27365/ |