Antimicrobial evaluation of hibiscus rosa sinensis plant extract against escherichia coli / Dhiya Adilia Roslan

Medicinal plant play many significant roles in treating various kind of disease and act as antimicrobial agent as it claimed to have antimicrobial compounds in various parts of plant. The objective of this research is to compare the antimicrobial activity from flower, stem and leaves of Hibiscus ro...

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Main Author: Roslan, Dhiya Adilia
Format: Student Project
Language:English
Published: Faculty of Applied Sciences 2016
Subjects:
Online Access:https://ir.uitm.edu.my/id/eprint/25144/
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author Roslan, Dhiya Adilia
author_facet Roslan, Dhiya Adilia
author_sort Roslan, Dhiya Adilia
building UiTM Institutional Repository
collection Online Access
description Medicinal plant play many significant roles in treating various kind of disease and act as antimicrobial agent as it claimed to have antimicrobial compounds in various parts of plant. The objective of this research is to compare the antimicrobial activity from flower, stem and leaves of Hibiscus rosa sinensis and to evaluate antimicrobial activity of methanol extracts of hibiscus towards food-borne pathogen Escherichia coli and Escherichia coli 0157:H7. In this preliminary investigation, the crude extract was prepared through maceration of dried flower, leaf and stems of the plant by methanolic solution. The antibacterial activity was screened by using Minimum Inhibitory Concentration (MIC) broth microdilution in and Minimum Bactericidal Concentration (MBC). The antibacterial activity were assessed by the turbidity of inoculum in MIC and the reduce of 99.9% or more growth of bacteria on plate from the initial inoculum for MBC. All methanolic extract showed antibacterial activity towards both Escherichia coli and Escherichia coli 0157:H7. The leaf extract of plant showed the highest antimicrobial activity against Escherichia coli, and the stem extract as the highest antimicrobial activity on Escherichia coli O 157 :H7.
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spelling uitm-251442019-08-29T06:46:17Z https://ir.uitm.edu.my/id/eprint/25144/ Antimicrobial evaluation of hibiscus rosa sinensis plant extract against escherichia coli / Dhiya Adilia Roslan Roslan, Dhiya Adilia Extraction (Chemistry) QR Microbiology Medicinal plant play many significant roles in treating various kind of disease and act as antimicrobial agent as it claimed to have antimicrobial compounds in various parts of plant. The objective of this research is to compare the antimicrobial activity from flower, stem and leaves of Hibiscus rosa sinensis and to evaluate antimicrobial activity of methanol extracts of hibiscus towards food-borne pathogen Escherichia coli and Escherichia coli 0157:H7. In this preliminary investigation, the crude extract was prepared through maceration of dried flower, leaf and stems of the plant by methanolic solution. The antibacterial activity was screened by using Minimum Inhibitory Concentration (MIC) broth microdilution in and Minimum Bactericidal Concentration (MBC). The antibacterial activity were assessed by the turbidity of inoculum in MIC and the reduce of 99.9% or more growth of bacteria on plate from the initial inoculum for MBC. All methanolic extract showed antibacterial activity towards both Escherichia coli and Escherichia coli 0157:H7. The leaf extract of plant showed the highest antimicrobial activity against Escherichia coli, and the stem extract as the highest antimicrobial activity on Escherichia coli O 157 :H7. Faculty of Applied Sciences 2016 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/25144/1/PPb_DHIYA%20ADILIA%20ROSLAN%20AS%20N%2016_5.pdf Roslan, Dhiya Adilia (2016) Antimicrobial evaluation of hibiscus rosa sinensis plant extract against escherichia coli / Dhiya Adilia Roslan. (2016) [Student Project] (Unpublished)
spellingShingle Extraction (Chemistry)
QR Microbiology
Roslan, Dhiya Adilia
Antimicrobial evaluation of hibiscus rosa sinensis plant extract against escherichia coli / Dhiya Adilia Roslan
title Antimicrobial evaluation of hibiscus rosa sinensis plant extract against escherichia coli / Dhiya Adilia Roslan
title_full Antimicrobial evaluation of hibiscus rosa sinensis plant extract against escherichia coli / Dhiya Adilia Roslan
title_fullStr Antimicrobial evaluation of hibiscus rosa sinensis plant extract against escherichia coli / Dhiya Adilia Roslan
title_full_unstemmed Antimicrobial evaluation of hibiscus rosa sinensis plant extract against escherichia coli / Dhiya Adilia Roslan
title_short Antimicrobial evaluation of hibiscus rosa sinensis plant extract against escherichia coli / Dhiya Adilia Roslan
title_sort antimicrobial evaluation of hibiscus rosa sinensis plant extract against escherichia coli / dhiya adilia roslan
topic Extraction (Chemistry)
QR Microbiology
url https://ir.uitm.edu.my/id/eprint/25144/