Cancer pharmacogenomics: the relevance of genetic profile in optimization drug therapy for azathiopurine and 5-fluorouracil / Prof Madya Dr Teh Lay Kek and Prof Dr Mohd Zaki Salleh
Introduction: Pharmacogenomic studies contribute to genetic information in preventing severe side effects of drugs. Genetic polymorphisms in drug metabolizing enzymes such as dihydropyrimidine dehydrogenase (DPD) had been associated with variable clinical outcomes in many commonly prescribed chemoth...
| Main Authors: | , |
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| Format: | Research Reports |
| Language: | English |
| Published: |
Research Management Institute (RMI)
2010
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| Online Access: | https://ir.uitm.edu.my/id/eprint/24808/ |
| _version_ | 1848806089747857408 |
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| author | Teh, Lay Kek Salleh, Mohd Zaki |
| author_facet | Teh, Lay Kek Salleh, Mohd Zaki |
| author_sort | Teh, Lay Kek |
| building | UiTM Institutional Repository |
| collection | Online Access |
| description | Introduction: Pharmacogenomic studies contribute to genetic information in preventing severe side effects of drugs. Genetic polymorphisms in drug metabolizing enzymes such as dihydropyrimidine dehydrogenase (DPD) had been associated with variable clinical outcomes in many commonly prescribed chemotherapy drugs including 5-Fluorouracil and irinotecan. Objectives: The review of literature had shed lights to the importance and possible impact of genetic polymorphism of DPYD and TPMT in individualization of drug therapy for 5-FU and thiopurines. However, there were no data reported for Malaysian. Current study thus aimed to explore the role of pharmacogenetics in personalized medicine in our own population. Materials and methods: Genotyping methods for DPYD and TPMT were developed using dHPLC and allele specific PCR respectively. 5-FU levels were measured in colorectal cancer patients using developed method. DNA from healthy volunteers and patients were screened. Results: Genotyping of DPYD had detected one reported mutation DPYD*5, two new mutations in exon 14 1823 T>C and 1827 G>A and one intronic reagion of exon 13, 13 IVS-11G>A with allele frequencies of 14.5%, .9.1%, 9.1% and 0.9% respectively. Genotyping for TPMT revealed 7 (7%) to be heterozygous for TPMT variant alleles. The predominant allele detected is TPMT*3C and is in concordance with previous studies done on Southeast Asian populations. |
| first_indexed | 2025-11-14T22:21:23Z |
| format | Research Reports |
| id | uitm-24808 |
| institution | Universiti Teknologi MARA |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T22:21:23Z |
| publishDate | 2010 |
| publisher | Research Management Institute (RMI) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uitm-248082024-11-28T07:31:16Z https://ir.uitm.edu.my/id/eprint/24808/ Cancer pharmacogenomics: the relevance of genetic profile in optimization drug therapy for azathiopurine and 5-fluorouracil / Prof Madya Dr Teh Lay Kek and Prof Dr Mohd Zaki Salleh Teh, Lay Kek Salleh, Mohd Zaki Cancer Introduction: Pharmacogenomic studies contribute to genetic information in preventing severe side effects of drugs. Genetic polymorphisms in drug metabolizing enzymes such as dihydropyrimidine dehydrogenase (DPD) had been associated with variable clinical outcomes in many commonly prescribed chemotherapy drugs including 5-Fluorouracil and irinotecan. Objectives: The review of literature had shed lights to the importance and possible impact of genetic polymorphism of DPYD and TPMT in individualization of drug therapy for 5-FU and thiopurines. However, there were no data reported for Malaysian. Current study thus aimed to explore the role of pharmacogenetics in personalized medicine in our own population. Materials and methods: Genotyping methods for DPYD and TPMT were developed using dHPLC and allele specific PCR respectively. 5-FU levels were measured in colorectal cancer patients using developed method. DNA from healthy volunteers and patients were screened. Results: Genotyping of DPYD had detected one reported mutation DPYD*5, two new mutations in exon 14 1823 T>C and 1827 G>A and one intronic reagion of exon 13, 13 IVS-11G>A with allele frequencies of 14.5%, .9.1%, 9.1% and 0.9% respectively. Genotyping for TPMT revealed 7 (7%) to be heterozygous for TPMT variant alleles. The predominant allele detected is TPMT*3C and is in concordance with previous studies done on Southeast Asian populations. Research Management Institute (RMI) 2010 Research Reports NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/24808/1/LP_TEH%20LAY%20KEK%20RMI%2010_5.pdf Teh, Lay Kek and Salleh, Mohd Zaki (2010) Cancer pharmacogenomics: the relevance of genetic profile in optimization drug therapy for azathiopurine and 5-fluorouracil / Prof Madya Dr Teh Lay Kek and Prof Dr Mohd Zaki Salleh. (2010) [Research Reports] <http://terminalib.uitm.edu.my/24808.pdf> (Unpublished) |
| spellingShingle | Cancer Teh, Lay Kek Salleh, Mohd Zaki Cancer pharmacogenomics: the relevance of genetic profile in optimization drug therapy for azathiopurine and 5-fluorouracil / Prof Madya Dr Teh Lay Kek and Prof Dr Mohd Zaki Salleh |
| title | Cancer pharmacogenomics: the relevance of genetic profile in optimization drug therapy for azathiopurine and 5-fluorouracil / Prof Madya Dr Teh Lay Kek and Prof Dr Mohd Zaki Salleh |
| title_full | Cancer pharmacogenomics: the relevance of genetic profile in optimization drug therapy for azathiopurine and 5-fluorouracil / Prof Madya Dr Teh Lay Kek and Prof Dr Mohd Zaki Salleh |
| title_fullStr | Cancer pharmacogenomics: the relevance of genetic profile in optimization drug therapy for azathiopurine and 5-fluorouracil / Prof Madya Dr Teh Lay Kek and Prof Dr Mohd Zaki Salleh |
| title_full_unstemmed | Cancer pharmacogenomics: the relevance of genetic profile in optimization drug therapy for azathiopurine and 5-fluorouracil / Prof Madya Dr Teh Lay Kek and Prof Dr Mohd Zaki Salleh |
| title_short | Cancer pharmacogenomics: the relevance of genetic profile in optimization drug therapy for azathiopurine and 5-fluorouracil / Prof Madya Dr Teh Lay Kek and Prof Dr Mohd Zaki Salleh |
| title_sort | cancer pharmacogenomics: the relevance of genetic profile in optimization drug therapy for azathiopurine and 5-fluorouracil / prof madya dr teh lay kek and prof dr mohd zaki salleh |
| topic | Cancer |
| url | https://ir.uitm.edu.my/id/eprint/24808/ |