Characterization of cellulases by aspergillus fumigatus using oil palm frond through solid state fermentation / Nurul Fatihah Mohd Zaid
There are lacks of microorganisms that can produce sufficient amount of three types of cellulases to break down the crystalline cellulose into glucose from the cellulosic wastes generated by agricultural and industrial activities. This study aims to characterize the thermostable enzyme produced by A...
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| Format: | Student Project |
| Language: | English |
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Faculty of Applied Sciences
2015
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| Online Access: | https://ir.uitm.edu.my/id/eprint/24079/ |
| _version_ | 1848805892629200896 |
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| author | Mohd Zaid, Nurul Fatihah |
| author_facet | Mohd Zaid, Nurul Fatihah |
| author_sort | Mohd Zaid, Nurul Fatihah |
| building | UiTM Institutional Repository |
| collection | Online Access |
| description | There are lacks of microorganisms that can produce sufficient amount of three types of cellulases to break down the crystalline cellulose into glucose from the cellulosic wastes generated by agricultural and industrial activities. This study aims to characterize the thermostable enzyme produced by Aspergillus fumigatus (previously isolated) in solid state fermentation using oil palm frond. The thermostable cellulase enzyme activities characterized by CMCase and FPase activities were concurrently done after 3 days of fermentation. This fungi colony has grayish color and smooth walled surface through macroscopic observation whilst the structures of fungi such as vesicle, conidiophore, conidia and phialide were done by microscopic observation. CMCase assay showed higher enzyme production compared to FPase assay. The highest enzyme production for CMCase and FPase assays are 0.0535 U/mL and 0.0249 U/mL respectively. The enzyme production is low due to high temperature during fermentation and small amount of substrate has been used. The highest reducing sugar obtained is 0.865 μmole on day 9 for reducing sugar analysis. Reducing sugar analysis showed that enzymatic hydrolysis did occurred where the cellulases broke down the cellulose into sugars. This study has proved that Aspergillus fumigatus can be used to degrade cellulosic wastes into soluble sugar by-products. It is suggested to prolong the enzyme production period to observe the production trends of the enzymes and increase the amount of substrate to reduce the water content to obtain higher amount of sugar produce. |
| first_indexed | 2025-11-14T22:18:15Z |
| format | Student Project |
| id | uitm-24079 |
| institution | Universiti Teknologi MARA |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T22:18:15Z |
| publishDate | 2015 |
| publisher | Faculty of Applied Sciences |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uitm-240792019-05-08T01:07:55Z https://ir.uitm.edu.my/id/eprint/24079/ Characterization of cellulases by aspergillus fumigatus using oil palm frond through solid state fermentation / Nurul Fatihah Mohd Zaid Mohd Zaid, Nurul Fatihah QR Microbiology Bacteria There are lacks of microorganisms that can produce sufficient amount of three types of cellulases to break down the crystalline cellulose into glucose from the cellulosic wastes generated by agricultural and industrial activities. This study aims to characterize the thermostable enzyme produced by Aspergillus fumigatus (previously isolated) in solid state fermentation using oil palm frond. The thermostable cellulase enzyme activities characterized by CMCase and FPase activities were concurrently done after 3 days of fermentation. This fungi colony has grayish color and smooth walled surface through macroscopic observation whilst the structures of fungi such as vesicle, conidiophore, conidia and phialide were done by microscopic observation. CMCase assay showed higher enzyme production compared to FPase assay. The highest enzyme production for CMCase and FPase assays are 0.0535 U/mL and 0.0249 U/mL respectively. The enzyme production is low due to high temperature during fermentation and small amount of substrate has been used. The highest reducing sugar obtained is 0.865 μmole on day 9 for reducing sugar analysis. Reducing sugar analysis showed that enzymatic hydrolysis did occurred where the cellulases broke down the cellulose into sugars. This study has proved that Aspergillus fumigatus can be used to degrade cellulosic wastes into soluble sugar by-products. It is suggested to prolong the enzyme production period to observe the production trends of the enzymes and increase the amount of substrate to reduce the water content to obtain higher amount of sugar produce. Faculty of Applied Sciences 2015 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/24079/1/PPb_NURUL%20FATIHAH%20MOHD%20ZAID%20AS%20N%2015_5.pdf Mohd Zaid, Nurul Fatihah (2015) Characterization of cellulases by aspergillus fumigatus using oil palm frond through solid state fermentation / Nurul Fatihah Mohd Zaid. (2015) [Student Project] <http://terminalib.uitm.edu.my/24079.pdf> (Submitted) |
| spellingShingle | QR Microbiology Bacteria Mohd Zaid, Nurul Fatihah Characterization of cellulases by aspergillus fumigatus using oil palm frond through solid state fermentation / Nurul Fatihah Mohd Zaid |
| title | Characterization of cellulases by aspergillus fumigatus using oil palm frond through solid state fermentation / Nurul Fatihah Mohd Zaid |
| title_full | Characterization of cellulases by aspergillus fumigatus using oil palm frond through solid state fermentation / Nurul Fatihah Mohd Zaid |
| title_fullStr | Characterization of cellulases by aspergillus fumigatus using oil palm frond through solid state fermentation / Nurul Fatihah Mohd Zaid |
| title_full_unstemmed | Characterization of cellulases by aspergillus fumigatus using oil palm frond through solid state fermentation / Nurul Fatihah Mohd Zaid |
| title_short | Characterization of cellulases by aspergillus fumigatus using oil palm frond through solid state fermentation / Nurul Fatihah Mohd Zaid |
| title_sort | characterization of cellulases by aspergillus fumigatus using oil palm frond through solid state fermentation / nurul fatihah mohd zaid |
| topic | QR Microbiology Bacteria |
| url | https://ir.uitm.edu.my/id/eprint/24079/ |