Isolation of compounds from ethyl acetate crude extracts in stem bark of pithecellobium jiringa / Norshakireen Khairul Shahrom

In this study, ethyl acetate (EA) crude extract of stem bark of Pithecellobium jiringa was subjected to fractional isolation and proceeds with antifungal activity determination. Five different EA fractions were successfully isolated and labelled as FI, F2, F3, F4, and F5. The isolation of EA crud...

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Main Author: Khairul Shahrom, Norshakireen
Format: Student Project
Language:English
Published: Faculty of Applied Sciences 2016
Subjects:
Online Access:https://ir.uitm.edu.my/id/eprint/23082/
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author Khairul Shahrom, Norshakireen
author_facet Khairul Shahrom, Norshakireen
author_sort Khairul Shahrom, Norshakireen
building UiTM Institutional Repository
collection Online Access
description In this study, ethyl acetate (EA) crude extract of stem bark of Pithecellobium jiringa was subjected to fractional isolation and proceeds with antifungal activity determination. Five different EA fractions were successfully isolated and labelled as FI, F2, F3, F4, and F5. The isolation of EA crude was achieved by using column chromatography method and analysed by thin layer chromatographic technique. Few compounds were identified based on TLC result on TLC plate. However, the compounds give negative response to antifungal test, which means, the compounds in the five fractions were not active towards antifungal test on T. mentagrophyte and C. albicans. There is no inhibition activities reported towards on these two fungi. The compounds then were been characterized by using GCMS and IR spectrometer. The major compound is unsaturated fatty acid which is hexadecanoic acid methyl ester.
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format Student Project
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institution Universiti Teknologi MARA
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language English
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publishDate 2016
publisher Faculty of Applied Sciences
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spelling uitm-230822019-02-11T09:21:58Z https://ir.uitm.edu.my/id/eprint/23082/ Isolation of compounds from ethyl acetate crude extracts in stem bark of pithecellobium jiringa / Norshakireen Khairul Shahrom Khairul Shahrom, Norshakireen Organic chemistry Extraction (Chemistry) In this study, ethyl acetate (EA) crude extract of stem bark of Pithecellobium jiringa was subjected to fractional isolation and proceeds with antifungal activity determination. Five different EA fractions were successfully isolated and labelled as FI, F2, F3, F4, and F5. The isolation of EA crude was achieved by using column chromatography method and analysed by thin layer chromatographic technique. Few compounds were identified based on TLC result on TLC plate. However, the compounds give negative response to antifungal test, which means, the compounds in the five fractions were not active towards antifungal test on T. mentagrophyte and C. albicans. There is no inhibition activities reported towards on these two fungi. The compounds then were been characterized by using GCMS and IR spectrometer. The major compound is unsaturated fatty acid which is hexadecanoic acid methyl ester. Faculty of Applied Sciences 2016 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/23082/1/PPb_NORSHAKIREEN%20KHAIRUL%20SHAHROM%20AS%20C%2016_5.PDF Khairul Shahrom, Norshakireen (2016) Isolation of compounds from ethyl acetate crude extracts in stem bark of pithecellobium jiringa / Norshakireen Khairul Shahrom. (2016) [Student Project] <http://terminalib.uitm.edu.my/23082.pdf> (Unpublished)
spellingShingle Organic chemistry
Extraction (Chemistry)
Khairul Shahrom, Norshakireen
Isolation of compounds from ethyl acetate crude extracts in stem bark of pithecellobium jiringa / Norshakireen Khairul Shahrom
title Isolation of compounds from ethyl acetate crude extracts in stem bark of pithecellobium jiringa / Norshakireen Khairul Shahrom
title_full Isolation of compounds from ethyl acetate crude extracts in stem bark of pithecellobium jiringa / Norshakireen Khairul Shahrom
title_fullStr Isolation of compounds from ethyl acetate crude extracts in stem bark of pithecellobium jiringa / Norshakireen Khairul Shahrom
title_full_unstemmed Isolation of compounds from ethyl acetate crude extracts in stem bark of pithecellobium jiringa / Norshakireen Khairul Shahrom
title_short Isolation of compounds from ethyl acetate crude extracts in stem bark of pithecellobium jiringa / Norshakireen Khairul Shahrom
title_sort isolation of compounds from ethyl acetate crude extracts in stem bark of pithecellobium jiringa / norshakireen khairul shahrom
topic Organic chemistry
Extraction (Chemistry)
url https://ir.uitm.edu.my/id/eprint/23082/