Characterization of Listeria monocytogenes isolated from foods / Zuraini Mat Issa, Maimunah Mustakim and Son Radu
Listeria monocytogenes is one of the major food-borne pathogen with an opportunistic character that is able to cause severe human listeriosis worldwide. The development of a surveillance and detection system concerning food safety in this country needs to be improved. Therefore, this study was condu...
| Main Authors: | , , |
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| Format: | Research Reports |
| Language: | English |
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Research Management Institute (RMI)
2012
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| Online Access: | https://ir.uitm.edu.my/id/eprint/21555/ |
| _version_ | 1848805276366405632 |
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| author | Mat Issa, Zuraini Mustakim, Maimunah Radu, Son |
| author_facet | Mat Issa, Zuraini Mustakim, Maimunah Radu, Son |
| author_sort | Mat Issa, Zuraini |
| building | UiTM Institutional Repository |
| collection | Online Access |
| description | Listeria monocytogenes is one of the major food-borne pathogen with an opportunistic character that is able to cause severe human listeriosis worldwide. The development of a surveillance and detection system concerning food safety in this country needs to be improved. Therefore, this study was conducted to provide a general baseline on the contamination of L. monocytogenes in various foods in the local markets in Selangor. A total of 140 raw and ready-to-eat food samples were analyzed by plating on selective PALCAM medium and the suspected L. monocytogenes colonies were confirmed at molecular level by MPN-PCR technique using primers specific for this food-borne pathogen. All of the 23 isolated strains yield a PCR product of 938 bp and 701 bp for 16s rRNA gene and hylA virulence gene respectively. The confirmed isolates were characterized for their intra-species discrimination according to reaction between antigen-antisera by serotyping technique and patterns generated by RAPD bands. Two screened RAPD primers demonstrating potentially useful banding patterns were selected and examined against L monocytogenes isolates. Positive isolates were evaluated for their susceptibility to eight commonly used antibiotic agents by using disc diffusion assay. L monocytogenes was detected in 8.57% of collected food samples which 33.3%, 25% and 13.3% were detected in burger, minced meat and sausage samples respectively. The highest resistances (100%) of L monocytogenes isolates were demonstrated against ampicillin and penicillin G antibiotics, whereas high susceptibility was showed toward streptomycin (100%). The molecular approaches used in this study provide highly sensitive and specific results which were very easy and fast to perform. These findings also suggested that the contamination of L monocytogenes in foods is relatively low. However, it highlighted the emergence of antibiotics multi-resistant Listeria in the present environment. |
| first_indexed | 2025-11-14T22:08:28Z |
| format | Research Reports |
| id | uitm-21555 |
| institution | Universiti Teknologi MARA |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T22:08:28Z |
| publishDate | 2012 |
| publisher | Research Management Institute (RMI) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uitm-215552018-10-01T08:29:11Z https://ir.uitm.edu.my/id/eprint/21555/ Characterization of Listeria monocytogenes isolated from foods / Zuraini Mat Issa, Maimunah Mustakim and Son Radu Mat Issa, Zuraini Mustakim, Maimunah Radu, Son Microbial ecology Listeria monocytogenes is one of the major food-borne pathogen with an opportunistic character that is able to cause severe human listeriosis worldwide. The development of a surveillance and detection system concerning food safety in this country needs to be improved. Therefore, this study was conducted to provide a general baseline on the contamination of L. monocytogenes in various foods in the local markets in Selangor. A total of 140 raw and ready-to-eat food samples were analyzed by plating on selective PALCAM medium and the suspected L. monocytogenes colonies were confirmed at molecular level by MPN-PCR technique using primers specific for this food-borne pathogen. All of the 23 isolated strains yield a PCR product of 938 bp and 701 bp for 16s rRNA gene and hylA virulence gene respectively. The confirmed isolates were characterized for their intra-species discrimination according to reaction between antigen-antisera by serotyping technique and patterns generated by RAPD bands. Two screened RAPD primers demonstrating potentially useful banding patterns were selected and examined against L monocytogenes isolates. Positive isolates were evaluated for their susceptibility to eight commonly used antibiotic agents by using disc diffusion assay. L monocytogenes was detected in 8.57% of collected food samples which 33.3%, 25% and 13.3% were detected in burger, minced meat and sausage samples respectively. The highest resistances (100%) of L monocytogenes isolates were demonstrated against ampicillin and penicillin G antibiotics, whereas high susceptibility was showed toward streptomycin (100%). The molecular approaches used in this study provide highly sensitive and specific results which were very easy and fast to perform. These findings also suggested that the contamination of L monocytogenes in foods is relatively low. However, it highlighted the emergence of antibiotics multi-resistant Listeria in the present environment. Research Management Institute (RMI) 2012 Research Reports NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/21555/1/LP_ZURAINA%20MAT%20ISSA%20RMI%2012_5.pdf Mat Issa, Zuraini and Mustakim, Maimunah and Radu, Son (2012) Characterization of Listeria monocytogenes isolated from foods / Zuraini Mat Issa, Maimunah Mustakim and Son Radu. (2012) [Research Reports] <http://terminalib.uitm.edu.my/21555.pdf> (Unpublished) |
| spellingShingle | Microbial ecology Mat Issa, Zuraini Mustakim, Maimunah Radu, Son Characterization of Listeria monocytogenes isolated from foods / Zuraini Mat Issa, Maimunah Mustakim and Son Radu |
| title | Characterization of Listeria monocytogenes isolated from foods / Zuraini Mat Issa, Maimunah Mustakim and Son Radu |
| title_full | Characterization of Listeria monocytogenes isolated from foods / Zuraini Mat Issa, Maimunah Mustakim and Son Radu |
| title_fullStr | Characterization of Listeria monocytogenes isolated from foods / Zuraini Mat Issa, Maimunah Mustakim and Son Radu |
| title_full_unstemmed | Characterization of Listeria monocytogenes isolated from foods / Zuraini Mat Issa, Maimunah Mustakim and Son Radu |
| title_short | Characterization of Listeria monocytogenes isolated from foods / Zuraini Mat Issa, Maimunah Mustakim and Son Radu |
| title_sort | characterization of listeria monocytogenes isolated from foods / zuraini mat issa, maimunah mustakim and son radu |
| topic | Microbial ecology |
| url | https://ir.uitm.edu.my/id/eprint/21555/ |