Relationship between timing of the first zygotic cleavage with cytoskeletal structures and amino acid metabolic profiles in vitrified mouse embryos / Wan Hafizah W. Jusof
Timing of the first zygotic cleavage has been used as a marker of embryo developmental competence and subsequent viability. Previous studies showed that embryos that cleaved early had higher developmental viability. However, the factors contributing to timing of the first zygotic cleavage are unknow...
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| Format: | Book Section |
| Language: | English |
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Institute of Graduate Studies, UiTM
2017
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| Online Access: | https://ir.uitm.edu.my/id/eprint/19856/ |
| _version_ | 1848804869510529024 |
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| author | W. Jusof, Wan Hafizah |
| author_facet | W. Jusof, Wan Hafizah |
| author_sort | W. Jusof, Wan Hafizah |
| building | UiTM Institutional Repository |
| collection | Online Access |
| description | Timing of the first zygotic cleavage has been used as a marker of embryo developmental competence and subsequent viability. Previous studies showed that embryos that cleaved early had higher developmental viability. However, the factors contributing to timing of the first zygotic cleavage are unknown. Energy production from mitochondria, nucleus and cytoskeletal organization might be some of the factors involved. Amino acid metabolic profiles might also relate with timing of the first zygotic cleavage as it has been reported to have significant relationships with embryo viability. Thus, the present study was designed to investigate the relationship between timing of the first zygotic cleavage, amino acid metabolic profiles, mitochondria, nucleus and cytoskeletal organization of mouse embryos with subsequent viability. Embryos were retrieved from superovulated ICR mice, 28 hours after hCG injection. At this point of time, 2-cell stage embryos were categorized as early-cleaving (EC), while zygotes with two pronuclei as late-cleaving (LC) embryos. Embryos were cultured overnight in M16 medium supplemented with 3% Bovine Serum Albumin (BSA) in a humidified carbon dioxide (CO2) incubator. For Experiment 1, both EC and LC embryos were divided into control and treatment groups. For control group, 2-cell stage embryos were cultured until the blastocyst stage. For treatment group, embryos were vitrified by EFS40 or EFS20/40 method for 1 hour and warmed. The vitrifiedwarmed embryos were cultured until the blastocyst stage. The number of surviving embryos and their development to the blastocyst were observed and counted… |
| first_indexed | 2025-11-14T22:02:00Z |
| format | Book Section |
| id | uitm-19856 |
| institution | Universiti Teknologi MARA |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T22:02:00Z |
| publishDate | 2017 |
| publisher | Institute of Graduate Studies, UiTM |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uitm-198562018-06-08T02:02:02Z https://ir.uitm.edu.my/id/eprint/19856/ Relationship between timing of the first zygotic cleavage with cytoskeletal structures and amino acid metabolic profiles in vitrified mouse embryos / Wan Hafizah W. Jusof W. Jusof, Wan Hafizah Malaysia Timing of the first zygotic cleavage has been used as a marker of embryo developmental competence and subsequent viability. Previous studies showed that embryos that cleaved early had higher developmental viability. However, the factors contributing to timing of the first zygotic cleavage are unknown. Energy production from mitochondria, nucleus and cytoskeletal organization might be some of the factors involved. Amino acid metabolic profiles might also relate with timing of the first zygotic cleavage as it has been reported to have significant relationships with embryo viability. Thus, the present study was designed to investigate the relationship between timing of the first zygotic cleavage, amino acid metabolic profiles, mitochondria, nucleus and cytoskeletal organization of mouse embryos with subsequent viability. Embryos were retrieved from superovulated ICR mice, 28 hours after hCG injection. At this point of time, 2-cell stage embryos were categorized as early-cleaving (EC), while zygotes with two pronuclei as late-cleaving (LC) embryos. Embryos were cultured overnight in M16 medium supplemented with 3% Bovine Serum Albumin (BSA) in a humidified carbon dioxide (CO2) incubator. For Experiment 1, both EC and LC embryos were divided into control and treatment groups. For control group, 2-cell stage embryos were cultured until the blastocyst stage. For treatment group, embryos were vitrified by EFS40 or EFS20/40 method for 1 hour and warmed. The vitrifiedwarmed embryos were cultured until the blastocyst stage. The number of surviving embryos and their development to the blastocyst were observed and counted… Institute of Graduate Studies, UiTM 2017 Book Section PeerReviewed text en https://ir.uitm.edu.my/id/eprint/19856/2/ABS_WAN%20HAFIZAH%20W.%20JUSOF%20TDRA%20VOL%2011%20IGS%2017.pdf W. Jusof, Wan Hafizah (2017) Relationship between timing of the first zygotic cleavage with cytoskeletal structures and amino acid metabolic profiles in vitrified mouse embryos / Wan Hafizah W. Jusof. (2017) In: The Doctoral Research Abstracts. IGS Biannual Publication, 11 (11). Institute of Graduate Studies, UiTM, Shah Alam. |
| spellingShingle | Malaysia W. Jusof, Wan Hafizah Relationship between timing of the first zygotic cleavage with cytoskeletal structures and amino acid metabolic profiles in vitrified mouse embryos / Wan Hafizah W. Jusof |
| title | Relationship between timing of the first zygotic cleavage with cytoskeletal structures and amino acid metabolic profiles in vitrified mouse embryos / Wan Hafizah W. Jusof |
| title_full | Relationship between timing of the first zygotic cleavage with cytoskeletal structures and amino acid metabolic profiles in vitrified mouse embryos / Wan Hafizah W. Jusof |
| title_fullStr | Relationship between timing of the first zygotic cleavage with cytoskeletal structures and amino acid metabolic profiles in vitrified mouse embryos / Wan Hafizah W. Jusof |
| title_full_unstemmed | Relationship between timing of the first zygotic cleavage with cytoskeletal structures and amino acid metabolic profiles in vitrified mouse embryos / Wan Hafizah W. Jusof |
| title_short | Relationship between timing of the first zygotic cleavage with cytoskeletal structures and amino acid metabolic profiles in vitrified mouse embryos / Wan Hafizah W. Jusof |
| title_sort | relationship between timing of the first zygotic cleavage with cytoskeletal structures and amino acid metabolic profiles in vitrified mouse embryos / wan hafizah w. jusof |
| topic | Malaysia |
| url | https://ir.uitm.edu.my/id/eprint/19856/ |