Effects of theaflavins rich fraction on pro-inflammatory markers and oxidative stress in stimulated endothelial cells / Remee Awang Jalil
Activation of endothelial cells (ECs) occurs early in atherogenesis leading to increased pro-inflammatory environment in the vessels. Oxidative stress also has been linked to atherogenesis, by promoting inflammatory environment and reduced nitric oxide availability in endothelium. Theaflavins rich f...
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| Format: | Thesis |
| Language: | English |
| Published: |
2016
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| Online Access: | https://ir.uitm.edu.my/id/eprint/17794/ |
| _version_ | 1848804360836874240 |
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| author | Awang Jalil, Remee |
| author_facet | Awang Jalil, Remee |
| author_sort | Awang Jalil, Remee |
| building | UiTM Institutional Repository |
| collection | Online Access |
| description | Activation of endothelial cells (ECs) occurs early in atherogenesis leading to increased pro-inflammatory environment in the vessels. Oxidative stress also has been linked to atherogenesis, by promoting inflammatory environment and reduced nitric oxide availability in endothelium. Theaflavins rich fractions (TFs-RF) from black tea, Camellia sinensis are believed to promote cardio protective benefits. However, their potential therapeutic roles as anti-inflammatory and anti-oxidative in atherogenesis are not well established. The objectives of this study were to investigate (i) the anti-inflammatory effects of TF-RFs on cytokines [Interleukin-6 (IL-6), and tumour necrosis factor alpha (TNF-a)] , cyclooxygenase-2 (COX-2), endothelial nitric oxide synthase (eNOS) and nitric oxide (NO), and (ii) the anti-oxidative effects of TFs-RF on reactive oxygen species (ROS) activity in stimulated human ECs. Cytotoxicity of TFs-RF (1.6 - 200 |xg/ml) towards Human umbilical vein endothelial cells (HUVECs) were assessed by using 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethoxyphenyl)- tetrazolium (MTS) assay. Confluent HUVECs were incubated with 10, 20, 30, 40 and 50 |ig/ml of TFs-RF together with lipopolysaccharides(LPS) for 16 hours. Culture medium were collected and measured for IL-6, TNF-a, COX-2 and eNOS protein expression and NO level by using Enzyme-linked immunosorbent assay (ELISA) and colorimetric assay respectively… |
| first_indexed | 2025-11-14T21:53:54Z |
| format | Thesis |
| id | uitm-17794 |
| institution | Universiti Teknologi MARA |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T21:53:54Z |
| publishDate | 2016 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uitm-177942024-11-18T03:11:17Z https://ir.uitm.edu.my/id/eprint/17794/ Effects of theaflavins rich fraction on pro-inflammatory markers and oxidative stress in stimulated endothelial cells / Remee Awang Jalil Awang Jalil, Remee Activation of endothelial cells (ECs) occurs early in atherogenesis leading to increased pro-inflammatory environment in the vessels. Oxidative stress also has been linked to atherogenesis, by promoting inflammatory environment and reduced nitric oxide availability in endothelium. Theaflavins rich fractions (TFs-RF) from black tea, Camellia sinensis are believed to promote cardio protective benefits. However, their potential therapeutic roles as anti-inflammatory and anti-oxidative in atherogenesis are not well established. The objectives of this study were to investigate (i) the anti-inflammatory effects of TF-RFs on cytokines [Interleukin-6 (IL-6), and tumour necrosis factor alpha (TNF-a)] , cyclooxygenase-2 (COX-2), endothelial nitric oxide synthase (eNOS) and nitric oxide (NO), and (ii) the anti-oxidative effects of TFs-RF on reactive oxygen species (ROS) activity in stimulated human ECs. Cytotoxicity of TFs-RF (1.6 - 200 |xg/ml) towards Human umbilical vein endothelial cells (HUVECs) were assessed by using 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethoxyphenyl)- tetrazolium (MTS) assay. Confluent HUVECs were incubated with 10, 20, 30, 40 and 50 |ig/ml of TFs-RF together with lipopolysaccharides(LPS) for 16 hours. Culture medium were collected and measured for IL-6, TNF-a, COX-2 and eNOS protein expression and NO level by using Enzyme-linked immunosorbent assay (ELISA) and colorimetric assay respectively… 2016-07 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/17794/7/17794.pdf Awang Jalil, Remee (2016) Effects of theaflavins rich fraction on pro-inflammatory markers and oxidative stress in stimulated endothelial cells / Remee Awang Jalil. (2016) Masters thesis, thesis, Universiti Teknologi MARA (UiTM). <http://terminalib.uitm.edu.my/17794.pdf> |
| spellingShingle | Awang Jalil, Remee Effects of theaflavins rich fraction on pro-inflammatory markers and oxidative stress in stimulated endothelial cells / Remee Awang Jalil |
| title | Effects of theaflavins rich fraction on pro-inflammatory markers and oxidative stress in stimulated endothelial cells / Remee Awang Jalil |
| title_full | Effects of theaflavins rich fraction on pro-inflammatory markers and oxidative stress in stimulated endothelial cells / Remee Awang Jalil |
| title_fullStr | Effects of theaflavins rich fraction on pro-inflammatory markers and oxidative stress in stimulated endothelial cells / Remee Awang Jalil |
| title_full_unstemmed | Effects of theaflavins rich fraction on pro-inflammatory markers and oxidative stress in stimulated endothelial cells / Remee Awang Jalil |
| title_short | Effects of theaflavins rich fraction on pro-inflammatory markers and oxidative stress in stimulated endothelial cells / Remee Awang Jalil |
| title_sort | effects of theaflavins rich fraction on pro-inflammatory markers and oxidative stress in stimulated endothelial cells / remee awang jalil |
| url | https://ir.uitm.edu.my/id/eprint/17794/ |