Kinetics of tapioca slurry saccharification process using immobilized multi-enzyme system enhanced with Sg. Sayong clay / Siti Noraida Abd Rahim, Alawi Sulaiman and Nurul Aini Edama
In this paper, immobilization of multi-enzyme using calcium alginate-clay beads for tapioca slurry saccharification process was carried out. The kinetics parameter and immobilization yield for alpha-amylase, glucoamylase, cellulose and multi-enzyme system were compared. The Michaelis constant, Km fo...
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| Format: | Article |
| Language: | English |
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Faculty of Mechanical Engineering Universiti Teknologi MARA (UiTM)
2014
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| Online Access: | https://ir.uitm.edu.my/id/eprint/17650/ |
| _version_ | 1848804323458285568 |
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| author | Abd Rahim, Siti Noraida Sulaiman, Alawi Edama, Nurul Aini |
| author_facet | Abd Rahim, Siti Noraida Sulaiman, Alawi Edama, Nurul Aini |
| author_sort | Abd Rahim, Siti Noraida |
| building | UiTM Institutional Repository |
| collection | Online Access |
| description | In this paper, immobilization of multi-enzyme using calcium alginate-clay beads for tapioca slurry saccharification process was carried out. The kinetics parameter and immobilization yield for alpha-amylase, glucoamylase, cellulose and multi-enzyme system were compared. The Michaelis constant, Km for free alpha-amylase, glucoamylase, cellulase and multi-enzyme were 2.0831,1.8326, 7.8592 and 3.5367 mg/mL, respectively. As for the immobilized system, the Km values were 3.1604, 2.1708, 9.2791 and 4.91176 mg/mL, respectively. The Km value for the immobilized multi-enzyme system was 1.39 times higher than free multi-enzyme system. This suggests that the immobilized multi-enzyme is a potential system for tapioca saccharification process due to affinity of enzyme to the substrate. For the immobilization yield, the yield for alpha-amylase, glucoamylase, cellulase and multi-enzyme were 51.19, 50.21, 40.68 and 53.71 %, respectively. The result showed the immobilized multi-enzyme had a higher yield compared to the single enzyme which could be explained due to the synergistic action of alpha-amylase, glucoamylase and cellulose enzymes. Therefore, it can be concluded that the use of immobilized multi-enzyme system had a very good potential for starch bioconversion into glucose. |
| first_indexed | 2025-11-14T21:53:19Z |
| format | Article |
| id | uitm-17650 |
| institution | Universiti Teknologi MARA |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T21:53:19Z |
| publishDate | 2014 |
| publisher | Faculty of Mechanical Engineering Universiti Teknologi MARA (UiTM) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uitm-176502020-03-24T07:21:24Z https://ir.uitm.edu.my/id/eprint/17650/ Kinetics of tapioca slurry saccharification process using immobilized multi-enzyme system enhanced with Sg. Sayong clay / Siti Noraida Abd Rahim, Alawi Sulaiman and Nurul Aini Edama jmeche Abd Rahim, Siti Noraida Sulaiman, Alawi Edama, Nurul Aini In this paper, immobilization of multi-enzyme using calcium alginate-clay beads for tapioca slurry saccharification process was carried out. The kinetics parameter and immobilization yield for alpha-amylase, glucoamylase, cellulose and multi-enzyme system were compared. The Michaelis constant, Km for free alpha-amylase, glucoamylase, cellulase and multi-enzyme were 2.0831,1.8326, 7.8592 and 3.5367 mg/mL, respectively. As for the immobilized system, the Km values were 3.1604, 2.1708, 9.2791 and 4.91176 mg/mL, respectively. The Km value for the immobilized multi-enzyme system was 1.39 times higher than free multi-enzyme system. This suggests that the immobilized multi-enzyme is a potential system for tapioca saccharification process due to affinity of enzyme to the substrate. For the immobilization yield, the yield for alpha-amylase, glucoamylase, cellulase and multi-enzyme were 51.19, 50.21, 40.68 and 53.71 %, respectively. The result showed the immobilized multi-enzyme had a higher yield compared to the single enzyme which could be explained due to the synergistic action of alpha-amylase, glucoamylase and cellulose enzymes. Therefore, it can be concluded that the use of immobilized multi-enzyme system had a very good potential for starch bioconversion into glucose. Faculty of Mechanical Engineering Universiti Teknologi MARA (UiTM) 2014 Article PeerReviewed text en https://ir.uitm.edu.my/id/eprint/17650/2/AJ_SITI%20NORAIDA%20ABD%20RAHIM%20JME%2014.pdf Abd Rahim, Siti Noraida and Sulaiman, Alawi and Edama, Nurul Aini (2014) Kinetics of tapioca slurry saccharification process using immobilized multi-enzyme system enhanced with Sg. Sayong clay / Siti Noraida Abd Rahim, Alawi Sulaiman and Nurul Aini Edama. (2014) Journal of Mechanical Engineering (JMechE) <https://ir.uitm.edu.my/view/publication/Journal_of_Mechanical_Engineering_=28JMechE=29.html>, 11 (2). pp. 67-77. ISSN 1823-5514 ; 2550-164X https://jmeche.uitm.edu.my/ |
| spellingShingle | Abd Rahim, Siti Noraida Sulaiman, Alawi Edama, Nurul Aini Kinetics of tapioca slurry saccharification process using immobilized multi-enzyme system enhanced with Sg. Sayong clay / Siti Noraida Abd Rahim, Alawi Sulaiman and Nurul Aini Edama |
| title | Kinetics of tapioca slurry saccharification process using immobilized multi-enzyme system enhanced with Sg. Sayong clay / Siti Noraida Abd Rahim, Alawi Sulaiman and Nurul Aini Edama |
| title_full | Kinetics of tapioca slurry saccharification process using immobilized multi-enzyme system enhanced with Sg. Sayong clay / Siti Noraida Abd Rahim, Alawi Sulaiman and Nurul Aini Edama |
| title_fullStr | Kinetics of tapioca slurry saccharification process using immobilized multi-enzyme system enhanced with Sg. Sayong clay / Siti Noraida Abd Rahim, Alawi Sulaiman and Nurul Aini Edama |
| title_full_unstemmed | Kinetics of tapioca slurry saccharification process using immobilized multi-enzyme system enhanced with Sg. Sayong clay / Siti Noraida Abd Rahim, Alawi Sulaiman and Nurul Aini Edama |
| title_short | Kinetics of tapioca slurry saccharification process using immobilized multi-enzyme system enhanced with Sg. Sayong clay / Siti Noraida Abd Rahim, Alawi Sulaiman and Nurul Aini Edama |
| title_sort | kinetics of tapioca slurry saccharification process using immobilized multi-enzyme system enhanced with sg. sayong clay / siti noraida abd rahim, alawi sulaiman and nurul aini edama |
| url | https://ir.uitm.edu.my/id/eprint/17650/ https://ir.uitm.edu.my/id/eprint/17650/ |