A review: protoplast isolation using different cellulase concentration / Nur Jama'iyah Roselan
One of the way to produce new plant is by using tissue culture technique. In tissue culture, protoplast isolation is the first step that should be done. There are two ways to isolate protoplast which are mechanical method and enzymatic method. However, this paper will study about protoplast isolatio...
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| Format: | Student Project |
| Language: | English |
| Published: |
Faculty of Plantation and Agrotechnology
2016
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| Online Access: | https://ir.uitm.edu.my/id/eprint/17443/ |
| _version_ | 1848804272805773312 |
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| author | Roselan, Nur Jama'iyah |
| author_facet | Roselan, Nur Jama'iyah |
| author_sort | Roselan, Nur Jama'iyah |
| building | UiTM Institutional Repository |
| collection | Online Access |
| description | One of the way to produce new plant is by using tissue culture technique. In tissue culture, protoplast isolation is the first step that should be done. There are two ways to isolate protoplast which are mechanical method and enzymatic method. However, this paper will study about protoplast isolation by using enzymatic method. Protoplast isolation have started during the early 1960 until now. With the development in technology and time, scientist have learn more about protoplast isolation technique but there is always room for improvement. There are various factors that can affect the yields and viability of the protoplast which are type of explants, isolation method and cellulase concentration. In this review paper, the focus will be on the effect of different enzyme concentration towards protoplast yield and viability especially in crops like oil palm, paddy and pineapple. It is clear that different explant require different cellulase concentration in order to give high yield and viability of protoplast. Further study should be done to identify which cellulase concentration are most suitable for each explant use. |
| first_indexed | 2025-11-14T21:52:30Z |
| format | Student Project |
| id | uitm-17443 |
| institution | Universiti Teknologi MARA |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T21:52:30Z |
| publishDate | 2016 |
| publisher | Faculty of Plantation and Agrotechnology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uitm-174432019-06-10T06:48:11Z https://ir.uitm.edu.my/id/eprint/17443/ A review: protoplast isolation using different cellulase concentration / Nur Jama'iyah Roselan Roselan, Nur Jama'iyah One of the way to produce new plant is by using tissue culture technique. In tissue culture, protoplast isolation is the first step that should be done. There are two ways to isolate protoplast which are mechanical method and enzymatic method. However, this paper will study about protoplast isolation by using enzymatic method. Protoplast isolation have started during the early 1960 until now. With the development in technology and time, scientist have learn more about protoplast isolation technique but there is always room for improvement. There are various factors that can affect the yields and viability of the protoplast which are type of explants, isolation method and cellulase concentration. In this review paper, the focus will be on the effect of different enzyme concentration towards protoplast yield and viability especially in crops like oil palm, paddy and pineapple. It is clear that different explant require different cellulase concentration in order to give high yield and viability of protoplast. Further study should be done to identify which cellulase concentration are most suitable for each explant use. Faculty of Plantation and Agrotechnology 2016 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/17443/2/PPb_NUR%20JAMA%27IYAH%20ROSELAN%20AT%2016_5.pdf Roselan, Nur Jama'iyah (2016) A review: protoplast isolation using different cellulase concentration / Nur Jama'iyah Roselan. (2016) [Student Project] <http://terminalib.uitm.edu.my/17443.pdf> (Unpublished) |
| spellingShingle | Roselan, Nur Jama'iyah A review: protoplast isolation using different cellulase concentration / Nur Jama'iyah Roselan |
| title | A review: protoplast isolation using different cellulase concentration / Nur Jama'iyah Roselan |
| title_full | A review: protoplast isolation using different cellulase concentration / Nur Jama'iyah Roselan |
| title_fullStr | A review: protoplast isolation using different cellulase concentration / Nur Jama'iyah Roselan |
| title_full_unstemmed | A review: protoplast isolation using different cellulase concentration / Nur Jama'iyah Roselan |
| title_short | A review: protoplast isolation using different cellulase concentration / Nur Jama'iyah Roselan |
| title_sort | review: protoplast isolation using different cellulase concentration / nur jama'iyah roselan |
| url | https://ir.uitm.edu.my/id/eprint/17443/ |