Palm oil γ-tocotrienol and α-tocopherol act as potent inducers in the immune response of mouse splenocytes in vitro / Narimah Abdul Hamid Hasani, Khalid bin Abdul Kadir and Wan Zurinah Wan Ngah
Vitamin E may have anti carcinogenesis effect in human and animal models via the mechanism of cell cycle arrest and enhancement of immune system. The cell-mediated immune inducing properties of palm oil vitamin E, for example, γ-tocotrienol and α-tocopherol, were investigated by measuring the mi...
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| Format: | Article |
| Language: | English |
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Research Management Institute (RMI)
2011
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| Online Access: | https://ir.uitm.edu.my/id/eprint/12942/ |
| _version_ | 1848803206045368320 |
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| author | Abdul Hamid Hasani, Narimah Abdul Kadir, Khalid Wan Ngah, Wan Zurinah |
| author_facet | Abdul Hamid Hasani, Narimah Abdul Kadir, Khalid Wan Ngah, Wan Zurinah |
| author_sort | Abdul Hamid Hasani, Narimah |
| building | UiTM Institutional Repository |
| collection | Online Access |
| description | Vitamin E may have anti carcinogenesis effect in human and animal models
via the mechanism of cell cycle arrest and enhancement of immune system.
The cell-mediated immune inducing properties of palm oil vitamin E, for
example, γ-tocotrienol and α-tocopherol, were investigated by measuring
the mitogenesis response of splenocytes, extracted from normal male Mus
musculus to splenic T-lymphocytes mitogens, phytohemagglutinin (PHA;
0.25 μg/mL) and concanavalin A (Con A; 1.0 μg/mL); and B-lymphocytes
mitogen i.e., lipopolysaccharide (LPS; 1.0 μg/mL). Both γ-tocotrienol
and α-tocopherol enhanced the cell proliferation of mitogen untreated
splenocytes as determined by 5-Bromo-2'-deoxy-uridine (BrdU) detection
method. Both compounds also enhanced the T-lymphocytes response to PHA
and Con A, as well as B-lymphocytes responses to LPS at all concentration
used (0-300 μM). γ-Tocotrienol was observed to affect cell proliferation
more than α-tocopherol. The uptake of γ-tocotrienol and α-tocopherol into
the splenocytes was determined by high performance liquid chromatography
(HPLC). γ-Tocotrienol was absorbed into the cells at markedly higher
levels than α-tocopherol with the ratio of 4.8 : 1 (p<0.01, n=4) at 300
μM of treatment. This may be the reason of the higher proliferation affect
of γ-tocotrienol as compared to α-tocopherol. In conclusion, we are
of the opinion that palm oil γ-tocotrienol and α-tocopherol are able to synergistically influence splenocytes proliferation thus enhancing the cellmediated
immune system. |
| first_indexed | 2025-11-14T21:35:33Z |
| format | Article |
| id | uitm-12942 |
| institution | Universiti Teknologi MARA |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T21:35:33Z |
| publishDate | 2011 |
| publisher | Research Management Institute (RMI) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uitm-129422016-05-30T02:25:23Z https://ir.uitm.edu.my/id/eprint/12942/ Palm oil γ-tocotrienol and α-tocopherol act as potent inducers in the immune response of mouse splenocytes in vitro / Narimah Abdul Hamid Hasani, Khalid bin Abdul Kadir and Wan Zurinah Wan Ngah srj Abdul Hamid Hasani, Narimah Abdul Kadir, Khalid Wan Ngah, Wan Zurinah Tocotrienol. Tocopherol. Vitamin E Immune response Vitamin E may have anti carcinogenesis effect in human and animal models via the mechanism of cell cycle arrest and enhancement of immune system. The cell-mediated immune inducing properties of palm oil vitamin E, for example, γ-tocotrienol and α-tocopherol, were investigated by measuring the mitogenesis response of splenocytes, extracted from normal male Mus musculus to splenic T-lymphocytes mitogens, phytohemagglutinin (PHA; 0.25 μg/mL) and concanavalin A (Con A; 1.0 μg/mL); and B-lymphocytes mitogen i.e., lipopolysaccharide (LPS; 1.0 μg/mL). Both γ-tocotrienol and α-tocopherol enhanced the cell proliferation of mitogen untreated splenocytes as determined by 5-Bromo-2'-deoxy-uridine (BrdU) detection method. Both compounds also enhanced the T-lymphocytes response to PHA and Con A, as well as B-lymphocytes responses to LPS at all concentration used (0-300 μM). γ-Tocotrienol was observed to affect cell proliferation more than α-tocopherol. The uptake of γ-tocotrienol and α-tocopherol into the splenocytes was determined by high performance liquid chromatography (HPLC). γ-Tocotrienol was absorbed into the cells at markedly higher levels than α-tocopherol with the ratio of 4.8 : 1 (p<0.01, n=4) at 300 μM of treatment. This may be the reason of the higher proliferation affect of γ-tocotrienol as compared to α-tocopherol. In conclusion, we are of the opinion that palm oil γ-tocotrienol and α-tocopherol are able to synergistically influence splenocytes proliferation thus enhancing the cellmediated immune system. Research Management Institute (RMI) 2011 Article PeerReviewed text en https://ir.uitm.edu.my/id/eprint/12942/1/AJ_%20NARIMAH%20ABDUL%20HAMID%20HASANI%20SRJ%2011%201.pdf Abdul Hamid Hasani, Narimah and Abdul Kadir, Khalid and Wan Ngah, Wan Zurinah (2011) Palm oil γ-tocotrienol and α-tocopherol act as potent inducers in the immune response of mouse splenocytes in vitro / Narimah Abdul Hamid Hasani, Khalid bin Abdul Kadir and Wan Zurinah Wan Ngah. (2011) Scientific Research Journal <https://ir.uitm.edu.my/view/publication/Scientific_Research_Journal.html>, 8 (1). pp. 17-28. ISSN 1675-7009 https://srj.uitm.edu.my/ |
| spellingShingle | Tocotrienol. Tocopherol. Vitamin E Immune response Abdul Hamid Hasani, Narimah Abdul Kadir, Khalid Wan Ngah, Wan Zurinah Palm oil γ-tocotrienol and α-tocopherol act as potent inducers in the immune response of mouse splenocytes in vitro / Narimah Abdul Hamid Hasani, Khalid bin Abdul Kadir and Wan Zurinah Wan Ngah |
| title | Palm oil γ-tocotrienol and α-tocopherol act as potent inducers in the immune response of mouse splenocytes in vitro / Narimah Abdul Hamid Hasani, Khalid bin Abdul Kadir and Wan Zurinah Wan Ngah |
| title_full | Palm oil γ-tocotrienol and α-tocopherol act as potent inducers in the immune response of mouse splenocytes in vitro / Narimah Abdul Hamid Hasani, Khalid bin Abdul Kadir and Wan Zurinah Wan Ngah |
| title_fullStr | Palm oil γ-tocotrienol and α-tocopherol act as potent inducers in the immune response of mouse splenocytes in vitro / Narimah Abdul Hamid Hasani, Khalid bin Abdul Kadir and Wan Zurinah Wan Ngah |
| title_full_unstemmed | Palm oil γ-tocotrienol and α-tocopherol act as potent inducers in the immune response of mouse splenocytes in vitro / Narimah Abdul Hamid Hasani, Khalid bin Abdul Kadir and Wan Zurinah Wan Ngah |
| title_short | Palm oil γ-tocotrienol and α-tocopherol act as potent inducers in the immune response of mouse splenocytes in vitro / Narimah Abdul Hamid Hasani, Khalid bin Abdul Kadir and Wan Zurinah Wan Ngah |
| title_sort | palm oil γ-tocotrienol and α-tocopherol act as potent inducers in the immune response of mouse splenocytes in vitro / narimah abdul hamid hasani, khalid bin abdul kadir and wan zurinah wan ngah |
| topic | Tocotrienol. Tocopherol. Vitamin E Immune response |
| url | https://ir.uitm.edu.my/id/eprint/12942/ https://ir.uitm.edu.my/id/eprint/12942/ |