Functional and antioxidant properties of angelwing clam (Pholas Orientalis) hydrolysate produced using alcalase / Normah Ismail, Juliana Mahmod and Awatif Khairul Fatihin Mustafa Kamal

In this study, Hydrolysate from angelwing clam (Pholas orientalis) was produced at 0, 1, 2 and 3 hrs and E/S ratio of 0.5 and 3% using alcalase where the pH and temperature were kept constant at pH 8.5 and 60°C, respectively. The hydrolysates were analysed for antioxidant and functional properties s...

Full description

Bibliographic Details
Main Authors: Ismail, Normah, Mahmod, Juliana, Mustafa Kamal, Awatif Khairul Fatihin
Format: Article
Language:English
Published: Research Management Institute (RMI) 2014
Subjects:
Online Access:https://ir.uitm.edu.my/id/eprint/12794/
_version_ 1848803181378666496
author Ismail, Normah
Mahmod, Juliana
Mustafa Kamal, Awatif Khairul Fatihin
author_facet Ismail, Normah
Mahmod, Juliana
Mustafa Kamal, Awatif Khairul Fatihin
author_sort Ismail, Normah
building UiTM Institutional Repository
collection Online Access
description In this study, Hydrolysate from angelwing clam (Pholas orientalis) was produced at 0, 1, 2 and 3 hrs and E/S ratio of 0.5 and 3% using alcalase where the pH and temperature were kept constant at pH 8.5 and 60°C, respectively. The hydrolysates were analysed for antioxidant and functional properties such as solubility, emulsifying properties and water and oil holding capacity. Degree of hydrolysis (DH), yield, functional and antioxidant properties were influenced by the hydrolysis time and E/S ratio. Higher enzyme concentration (E/S 3%) and longer hydrolysis time increased the DH. Yield was higher at E/S 3% but reduced with hydrolysis time. Longer hydrolysis time produced more soluble hydrolysate and higher metal chelating activity but lower in emulsifying properties and DPPH activity. Higher enzyme concentration resulted in increase only in solubility and metal chelating activity. This study revealed that enzymatic hydrolysis using alcalase should be performed at shorter hydrolysis time using intermediate concentration of enzyme (E/S between 0.5 to 3%) in order to produce angelwing clam hydrolysate with collectively good functional and antioxidant properties.
first_indexed 2025-11-14T21:35:10Z
format Article
id uitm-12794
institution Universiti Teknologi MARA
institution_category Local University
language English
last_indexed 2025-11-14T21:35:10Z
publishDate 2014
publisher Research Management Institute (RMI)
recordtype eprints
repository_type Digital Repository
spelling uitm-127942023-04-27T07:44:51Z https://ir.uitm.edu.my/id/eprint/12794/ Functional and antioxidant properties of angelwing clam (Pholas Orientalis) hydrolysate produced using alcalase / Normah Ismail, Juliana Mahmod and Awatif Khairul Fatihin Mustafa Kamal srj Ismail, Normah Mahmod, Juliana Mustafa Kamal, Awatif Khairul Fatihin Physical and theoretical chemistry Clams In this study, Hydrolysate from angelwing clam (Pholas orientalis) was produced at 0, 1, 2 and 3 hrs and E/S ratio of 0.5 and 3% using alcalase where the pH and temperature were kept constant at pH 8.5 and 60°C, respectively. The hydrolysates were analysed for antioxidant and functional properties such as solubility, emulsifying properties and water and oil holding capacity. Degree of hydrolysis (DH), yield, functional and antioxidant properties were influenced by the hydrolysis time and E/S ratio. Higher enzyme concentration (E/S 3%) and longer hydrolysis time increased the DH. Yield was higher at E/S 3% but reduced with hydrolysis time. Longer hydrolysis time produced more soluble hydrolysate and higher metal chelating activity but lower in emulsifying properties and DPPH activity. Higher enzyme concentration resulted in increase only in solubility and metal chelating activity. This study revealed that enzymatic hydrolysis using alcalase should be performed at shorter hydrolysis time using intermediate concentration of enzyme (E/S between 0.5 to 3%) in order to produce angelwing clam hydrolysate with collectively good functional and antioxidant properties. Research Management Institute (RMI) 2014 Article PeerReviewed text en https://ir.uitm.edu.my/id/eprint/12794/2/12794.pdf Ismail, Normah and Mahmod, Juliana and Mustafa Kamal, Awatif Khairul Fatihin (2014) Functional and antioxidant properties of angelwing clam (Pholas Orientalis) hydrolysate produced using alcalase / Normah Ismail, Juliana Mahmod and Awatif Khairul Fatihin Mustafa Kamal. (2014) Scientific Research Journal <https://ir.uitm.edu.my/view/publication/Scientific_Research_Journal.html>, 11 (1). pp. 29-49. ISSN 1675-7009 https://srj.uitm.edu.my/
spellingShingle Physical and theoretical chemistry
Clams
Ismail, Normah
Mahmod, Juliana
Mustafa Kamal, Awatif Khairul Fatihin
Functional and antioxidant properties of angelwing clam (Pholas Orientalis) hydrolysate produced using alcalase / Normah Ismail, Juliana Mahmod and Awatif Khairul Fatihin Mustafa Kamal
title Functional and antioxidant properties of angelwing clam (Pholas Orientalis) hydrolysate produced using alcalase / Normah Ismail, Juliana Mahmod and Awatif Khairul Fatihin Mustafa Kamal
title_full Functional and antioxidant properties of angelwing clam (Pholas Orientalis) hydrolysate produced using alcalase / Normah Ismail, Juliana Mahmod and Awatif Khairul Fatihin Mustafa Kamal
title_fullStr Functional and antioxidant properties of angelwing clam (Pholas Orientalis) hydrolysate produced using alcalase / Normah Ismail, Juliana Mahmod and Awatif Khairul Fatihin Mustafa Kamal
title_full_unstemmed Functional and antioxidant properties of angelwing clam (Pholas Orientalis) hydrolysate produced using alcalase / Normah Ismail, Juliana Mahmod and Awatif Khairul Fatihin Mustafa Kamal
title_short Functional and antioxidant properties of angelwing clam (Pholas Orientalis) hydrolysate produced using alcalase / Normah Ismail, Juliana Mahmod and Awatif Khairul Fatihin Mustafa Kamal
title_sort functional and antioxidant properties of angelwing clam (pholas orientalis) hydrolysate produced using alcalase / normah ismail, juliana mahmod and awatif khairul fatihin mustafa kamal
topic Physical and theoretical chemistry
Clams
url https://ir.uitm.edu.my/id/eprint/12794/
https://ir.uitm.edu.my/id/eprint/12794/