Characterization and purification of protease extracted from two maturity stages of ‘Noni’ (Morinda citrifolia L.) fruit / Normah Ismail and Nurulain Abd Razak
Protease was extracted from two maturity stages of noni fruits (Morinda citrifolia L.), unripe (stage 1) and ripe (stage 5). The crude extract was partially purified by acetone precipitation method followed by dialysis, gel filtration chromatography and freeze drying. Protein concentrations, proteo...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Research Management Institute (RMI)
2014
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| Online Access: | https://ir.uitm.edu.my/id/eprint/12429/ |
| _version_ | 1848803154291851264 |
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| author | Ismail, Normah Abd Razak, Nurulain |
| author_facet | Ismail, Normah Abd Razak, Nurulain |
| author_sort | Ismail, Normah |
| building | UiTM Institutional Repository |
| collection | Online Access |
| description | Protease was extracted from two maturity stages of noni fruits (Morinda citrifolia L.), unripe (stage 1) and ripe (stage 5). The crude extract was partially purified by acetone precipitation method followed by dialysis, gel filtration chromatography and freeze drying. Protein
concentrations, proteolytic activity, molecular weight distribution, pH stability, temperature stability and storage efficiency of the resulting protease were evaluated. The unripe and ripe noni fruit contains 0.65 and 0.35% protein, respectively. Molecular weight of the proteases from both stages ranged approximately between 3 to 28 kDa based on the SDS-PAGE results. The optimum
activity were at pH 7s and 6, temperatures of 40 and 50°C, respectively for proteases obtained from the unripe and ripe fruit. Analysis from the freeze dried protease indicated that protease from ripe noni fruits had higher protein concentration and specific activity compared to those from unripe fruit. However, it is more sensitive to pH and temperature and less stable during storage as it shows lower proteolytic activity compared to protease from unripe fruit. Based on its high proteolytic activity reaching up to 70.31 U/mg and storage stability (30% lost of activity), noni fruit could be an alternative source of plant protease. |
| first_indexed | 2025-11-14T21:34:44Z |
| format | Article |
| id | uitm-12429 |
| institution | Universiti Teknologi MARA |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T21:34:44Z |
| publishDate | 2014 |
| publisher | Research Management Institute (RMI) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uitm-124292023-04-27T08:27:50Z https://ir.uitm.edu.my/id/eprint/12429/ Characterization and purification of protease extracted from two maturity stages of ‘Noni’ (Morinda citrifolia L.) fruit / Normah Ismail and Nurulain Abd Razak srj Ismail, Normah Abd Razak, Nurulain Rubiaceae (Madder). Morinda citrifolia Proteolytic enzymes. Proteases Protease was extracted from two maturity stages of noni fruits (Morinda citrifolia L.), unripe (stage 1) and ripe (stage 5). The crude extract was partially purified by acetone precipitation method followed by dialysis, gel filtration chromatography and freeze drying. Protein concentrations, proteolytic activity, molecular weight distribution, pH stability, temperature stability and storage efficiency of the resulting protease were evaluated. The unripe and ripe noni fruit contains 0.65 and 0.35% protein, respectively. Molecular weight of the proteases from both stages ranged approximately between 3 to 28 kDa based on the SDS-PAGE results. The optimum activity were at pH 7s and 6, temperatures of 40 and 50°C, respectively for proteases obtained from the unripe and ripe fruit. Analysis from the freeze dried protease indicated that protease from ripe noni fruits had higher protein concentration and specific activity compared to those from unripe fruit. However, it is more sensitive to pH and temperature and less stable during storage as it shows lower proteolytic activity compared to protease from unripe fruit. Based on its high proteolytic activity reaching up to 70.31 U/mg and storage stability (30% lost of activity), noni fruit could be an alternative source of plant protease. Research Management Institute (RMI) 2014 Article PeerReviewed text en https://ir.uitm.edu.my/id/eprint/12429/2/12429.pdf Ismail, Normah and Abd Razak, Nurulain (2014) Characterization and purification of protease extracted from two maturity stages of ‘Noni’ (Morinda citrifolia L.) fruit / Normah Ismail and Nurulain Abd Razak. (2014) Scientific Research Journal <https://ir.uitm.edu.my/view/publication/Scientific_Research_Journal.html>, 11 (2). pp. 1-16. ISSN 1675-7009 https://srj.uitm.edu.my/ |
| spellingShingle | Rubiaceae (Madder). Morinda citrifolia Proteolytic enzymes. Proteases Ismail, Normah Abd Razak, Nurulain Characterization and purification of protease extracted from two maturity stages of ‘Noni’ (Morinda citrifolia L.) fruit / Normah Ismail and Nurulain Abd Razak |
| title | Characterization and purification of protease extracted from two maturity stages of ‘Noni’ (Morinda citrifolia L.) fruit / Normah Ismail and Nurulain Abd Razak |
| title_full | Characterization and purification of protease extracted from two maturity stages of ‘Noni’ (Morinda citrifolia L.) fruit / Normah Ismail and Nurulain Abd Razak |
| title_fullStr | Characterization and purification of protease extracted from two maturity stages of ‘Noni’ (Morinda citrifolia L.) fruit / Normah Ismail and Nurulain Abd Razak |
| title_full_unstemmed | Characterization and purification of protease extracted from two maturity stages of ‘Noni’ (Morinda citrifolia L.) fruit / Normah Ismail and Nurulain Abd Razak |
| title_short | Characterization and purification of protease extracted from two maturity stages of ‘Noni’ (Morinda citrifolia L.) fruit / Normah Ismail and Nurulain Abd Razak |
| title_sort | characterization and purification of protease extracted from two maturity stages of ‘noni’ (morinda citrifolia l.) fruit / normah ismail and nurulain abd razak |
| topic | Rubiaceae (Madder). Morinda citrifolia Proteolytic enzymes. Proteases |
| url | https://ir.uitm.edu.my/id/eprint/12429/ https://ir.uitm.edu.my/id/eprint/12429/ |