Preparation and characterization of PEGylated C18 fatty acids/Anti-SNAP25 antibody-targeted Liposomes
Background: Unsaturated C18 fatty acids, such as oleic acid (L1), linoleic acid (L2), and linolenic acid (L3), are a good choice of lipids to prepare liposomes. They are inexpensive, biocompatible, nontoxic, and readily available compared with phospholipids. Moreover, cis-double bonds of unsaturated...
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| Language: | English |
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Bentham Science
2019
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| Online Access: | http://eprints.sunway.edu.my/992/ http://eprints.sunway.edu.my/992/1/Gew%20Preparation%20PDF.pdf |
| _version_ | 1848801965793869824 |
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| author | Gew, Lai Ti * Eh, Suk Vicit Rizal Misni Misran, |
| author_facet | Gew, Lai Ti * Eh, Suk Vicit Rizal Misni Misran, |
| author_sort | Gew, Lai Ti * |
| building | SU Institutional Repository |
| collection | Online Access |
| description | Background: Unsaturated C18 fatty acids, such as oleic acid (L1), linoleic acid (L2), and linolenic acid (L3), are a good choice of lipids to prepare liposomes. They are inexpensive, biocompatible, nontoxic, and readily available compared with phospholipids. Moreover, cis-double bonds of unsaturated fatty acids prevent the packing of molecules which increases membrane fluidity in liposomes making them a good choice of starting materials to prepare liposomes.
Objective: Unsaturated C18 fatty acid liposomes, as well as their PEGylated and nonPEGylated antibody-targeted liposomes, were prepared and characterized.
Method: The particle size and zeta potential of the prepared liposomes (1 mM, pH = 7.4) for 28 and 14 days, respectively, were monitored and characterized. Membrane-bound antibodies Anti-SNAP25 (AS25) and DOPE PEG2000 (DP) were conjugated to pure C18 fatty acid liposomes to achieve stable fatty acid formulations.
Results: The mean particle sizes of pure L1, L2, and L3 liposome solutions were 125, 129, and 122 nm respectively, while their polydispersity index values were 0.28, 0.21, and 0.40 respectively. A large negative zeta potential value of 45 mV was observed due to anionic carboxylate head-group of pure liposomes. The incorporation of AS25 into L1/DP, L2/DP, and L3/DP liposome solutions stabilized their mean particle size and zeta potential measurements over 28 and 14 days, respectively.
Conclusion: L1/DP/AS25 was found to be the most stable PEGylated antibody-targeted liposome system because its particle size remained between 90 and 125 nm in 28 days. Transmission electron microscopy observations also supported the incorporation of AS25 and DP on the membrane surface as predicted. |
| first_indexed | 2025-11-14T21:15:50Z |
| format | Article |
| id | sunway-992 |
| institution | Sunway University |
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| language | English |
| last_indexed | 2025-11-14T21:15:50Z |
| publishDate | 2019 |
| publisher | Bentham Science |
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| spelling | sunway-9922020-07-06T03:32:17Z http://eprints.sunway.edu.my/992/ Preparation and characterization of PEGylated C18 fatty acids/Anti-SNAP25 antibody-targeted Liposomes Gew, Lai Ti * Eh, Suk Vicit Rizal Misni Misran, QH301 Biology Background: Unsaturated C18 fatty acids, such as oleic acid (L1), linoleic acid (L2), and linolenic acid (L3), are a good choice of lipids to prepare liposomes. They are inexpensive, biocompatible, nontoxic, and readily available compared with phospholipids. Moreover, cis-double bonds of unsaturated fatty acids prevent the packing of molecules which increases membrane fluidity in liposomes making them a good choice of starting materials to prepare liposomes. Objective: Unsaturated C18 fatty acid liposomes, as well as their PEGylated and nonPEGylated antibody-targeted liposomes, were prepared and characterized. Method: The particle size and zeta potential of the prepared liposomes (1 mM, pH = 7.4) for 28 and 14 days, respectively, were monitored and characterized. Membrane-bound antibodies Anti-SNAP25 (AS25) and DOPE PEG2000 (DP) were conjugated to pure C18 fatty acid liposomes to achieve stable fatty acid formulations. Results: The mean particle sizes of pure L1, L2, and L3 liposome solutions were 125, 129, and 122 nm respectively, while their polydispersity index values were 0.28, 0.21, and 0.40 respectively. A large negative zeta potential value of 45 mV was observed due to anionic carboxylate head-group of pure liposomes. The incorporation of AS25 into L1/DP, L2/DP, and L3/DP liposome solutions stabilized their mean particle size and zeta potential measurements over 28 and 14 days, respectively. Conclusion: L1/DP/AS25 was found to be the most stable PEGylated antibody-targeted liposome system because its particle size remained between 90 and 125 nm in 28 days. Transmission electron microscopy observations also supported the incorporation of AS25 and DP on the membrane surface as predicted. Bentham Science 2019 Article PeerReviewed text en cc_by_nc_4 http://eprints.sunway.edu.my/992/1/Gew%20Preparation%20PDF.pdf Gew, Lai Ti * and Eh, Suk Vicit Rizal and Misni Misran, (2019) Preparation and characterization of PEGylated C18 fatty acids/Anti-SNAP25 antibody-targeted Liposomes. Current Chemical Biology, 13 (2). pp. 129-139. ISSN 2212-7968 https://www.eurekaselect.com/165343/article doi:10.2174/2212796812666180912113156 |
| spellingShingle | QH301 Biology Gew, Lai Ti * Eh, Suk Vicit Rizal Misni Misran, Preparation and characterization of PEGylated C18 fatty acids/Anti-SNAP25 antibody-targeted Liposomes |
| title | Preparation and characterization of PEGylated C18 fatty acids/Anti-SNAP25 antibody-targeted Liposomes |
| title_full | Preparation and characterization of PEGylated C18 fatty acids/Anti-SNAP25 antibody-targeted Liposomes |
| title_fullStr | Preparation and characterization of PEGylated C18 fatty acids/Anti-SNAP25 antibody-targeted Liposomes |
| title_full_unstemmed | Preparation and characterization of PEGylated C18 fatty acids/Anti-SNAP25 antibody-targeted Liposomes |
| title_short | Preparation and characterization of PEGylated C18 fatty acids/Anti-SNAP25 antibody-targeted Liposomes |
| title_sort | preparation and characterization of pegylated c18 fatty acids/anti-snap25 antibody-targeted liposomes |
| topic | QH301 Biology |
| url | http://eprints.sunway.edu.my/992/ http://eprints.sunway.edu.my/992/ http://eprints.sunway.edu.my/992/ http://eprints.sunway.edu.my/992/1/Gew%20Preparation%20PDF.pdf |