Novel genetic variants of Hepatitis B Virus in fulminant hepatitis
Fulminant hepatitis (FH) is a life-threatening liver disease characterised by intense immune attack and massive liver cell death. The common precore stop codon mutation of hepatitis B virus (HBV), A1896, is frequently associated with FH, but lacks specificity. This study attempts to uncover all poss...
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| Format: | Article |
| Language: | English |
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Hindawi
2017
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| Online Access: | http://eprints.sunway.edu.my/871/ http://eprints.sunway.edu.my/871/1/Chook%20JB%202017%20Novel%20Genetic%20variants%20of%20fulminant%20HBV%20J%20Pathog.pdf |
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| author | Chook, Jack Bee * Ngeow, Yun Fong Tee, Kok Keng Peh, Suat Cheng * Mohamed, Rosmawati |
| author_facet | Chook, Jack Bee * Ngeow, Yun Fong Tee, Kok Keng Peh, Suat Cheng * Mohamed, Rosmawati |
| author_sort | Chook, Jack Bee * |
| building | SU Institutional Repository |
| collection | Online Access |
| description | Fulminant hepatitis (FH) is a life-threatening liver disease characterised by intense immune attack and massive liver cell death. The common precore stop codon mutation of hepatitis B virus (HBV), A1896, is frequently associated with FH, but lacks specificity. This study attempts to uncover all possible viral nucleotides that are specifically associated with FH through a compiled sequence analysis of FH and non-FH cases from acute infection. We retrieved 67 FH and 280 acute non-FH cases of hepatitis B from GenBank and applied support vector machine (SVM) model to seek candidate nucleotides highly predictive of FH. Six best candidates with top predictive accuracy, 92.5%, were used to build a SVM model; they are C2129 (85.3%), T720 (83.0%), Y2131 (82.4%), T2013 (82.1%),K2048 (82.1%), and A2512 (82.1%). This model gave a high specificity (99.3%), positive predictive value (95.6%), and negative
predictive value (92.1%), but only moderate sensitivity (64.2%).We successfully built a SVM model comprising six variants that are highly predictive and specific for FH: four in the core region and one each in the polymerase and the surface regions. These variants indicate that intracellular virion/core retention could play an important role in the progression to FH. |
| first_indexed | 2025-11-14T21:15:03Z |
| format | Article |
| id | sunway-871 |
| institution | Sunway University |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T21:15:03Z |
| publishDate | 2017 |
| publisher | Hindawi |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | sunway-8712019-04-30T08:24:20Z http://eprints.sunway.edu.my/871/ Novel genetic variants of Hepatitis B Virus in fulminant hepatitis Chook, Jack Bee * Ngeow, Yun Fong Tee, Kok Keng Peh, Suat Cheng * Mohamed, Rosmawati R Medicine (General) Fulminant hepatitis (FH) is a life-threatening liver disease characterised by intense immune attack and massive liver cell death. The common precore stop codon mutation of hepatitis B virus (HBV), A1896, is frequently associated with FH, but lacks specificity. This study attempts to uncover all possible viral nucleotides that are specifically associated with FH through a compiled sequence analysis of FH and non-FH cases from acute infection. We retrieved 67 FH and 280 acute non-FH cases of hepatitis B from GenBank and applied support vector machine (SVM) model to seek candidate nucleotides highly predictive of FH. Six best candidates with top predictive accuracy, 92.5%, were used to build a SVM model; they are C2129 (85.3%), T720 (83.0%), Y2131 (82.4%), T2013 (82.1%),K2048 (82.1%), and A2512 (82.1%). This model gave a high specificity (99.3%), positive predictive value (95.6%), and negative predictive value (92.1%), but only moderate sensitivity (64.2%).We successfully built a SVM model comprising six variants that are highly predictive and specific for FH: four in the core region and one each in the polymerase and the surface regions. These variants indicate that intracellular virion/core retention could play an important role in the progression to FH. Hindawi 2017-12-19 Article PeerReviewed text en http://eprints.sunway.edu.my/871/1/Chook%20JB%202017%20Novel%20Genetic%20variants%20of%20fulminant%20HBV%20J%20Pathog.pdf Chook, Jack Bee * and Ngeow, Yun Fong and Tee, Kok Keng and Peh, Suat Cheng * and Mohamed, Rosmawati (2017) Novel genetic variants of Hepatitis B Virus in fulminant hepatitis. Journal of Pathogens, 2017. pp. 1-6. ISSN 2090-3057 http://doi.org/10.1155/2017/1231204 doi:10.1155/2017/1231204 |
| spellingShingle | R Medicine (General) Chook, Jack Bee * Ngeow, Yun Fong Tee, Kok Keng Peh, Suat Cheng * Mohamed, Rosmawati Novel genetic variants of Hepatitis B Virus in fulminant hepatitis |
| title | Novel genetic variants of Hepatitis B Virus in fulminant hepatitis |
| title_full | Novel genetic variants of Hepatitis B Virus in fulminant hepatitis |
| title_fullStr | Novel genetic variants of Hepatitis B Virus in fulminant hepatitis |
| title_full_unstemmed | Novel genetic variants of Hepatitis B Virus in fulminant hepatitis |
| title_short | Novel genetic variants of Hepatitis B Virus in fulminant hepatitis |
| title_sort | novel genetic variants of hepatitis b virus in fulminant hepatitis |
| topic | R Medicine (General) |
| url | http://eprints.sunway.edu.my/871/ http://eprints.sunway.edu.my/871/ http://eprints.sunway.edu.my/871/ http://eprints.sunway.edu.my/871/1/Chook%20JB%202017%20Novel%20Genetic%20variants%20of%20fulminant%20HBV%20J%20Pathog.pdf |