Apoptosis in Acanthamoeba castellanii belonging to the T4 genotype
Here we describe features of apoptosis in unicellular Acanthamoeba castellanii belonging to the T4 genotype. When exposed to apoptosis-inducing compounds such as doxorubicin, A. castellanii trophozoites exhibited cell shrinkage and membrane blebbing as observed microscopically, DNA fragmentation usi...
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| Format: | Article |
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Wiley-VCH Verlag
2017
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| Online Access: | http://eprints.sunway.edu.my/494/ |
| _version_ | 1848801832493645824 |
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| author | Baig, Abdul Mannan Lalani, S.* Khan, Naveed Ahmed * |
| author_facet | Baig, Abdul Mannan Lalani, S.* Khan, Naveed Ahmed * |
| author_sort | Baig, Abdul Mannan |
| building | SU Institutional Repository |
| collection | Online Access |
| description | Here we describe features of apoptosis in unicellular Acanthamoeba castellanii belonging to the T4 genotype. When exposed to apoptosis-inducing compounds such as doxorubicin, A. castellanii trophozoites exhibited cell shrinkage and membrane blebbing as observed microscopically, DNA fragmentation using agarose gel electrophoresis, and phosphatidylserine (PS) externalization using annexinVimmunostaining. Overall,thesefindingssuggesttheexistenceofapoptosis inA.castellaniipossiblymediatedbyintrinsicapoptoticcascade.Furtherresearchin this field could provide avenues to selectively induce apoptosis in A. castellanii by triggering intrinsic apoptotic cascade. |
| first_indexed | 2025-11-14T21:13:43Z |
| format | Article |
| id | sunway-494 |
| institution | Sunway University |
| institution_category | Local University |
| last_indexed | 2025-11-14T21:13:43Z |
| publishDate | 2017 |
| publisher | Wiley-VCH Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | sunway-4942020-10-01T09:29:49Z http://eprints.sunway.edu.my/494/ Apoptosis in Acanthamoeba castellanii belonging to the T4 genotype Baig, Abdul Mannan Lalani, S.* Khan, Naveed Ahmed * Q Science (General) QR Microbiology Here we describe features of apoptosis in unicellular Acanthamoeba castellanii belonging to the T4 genotype. When exposed to apoptosis-inducing compounds such as doxorubicin, A. castellanii trophozoites exhibited cell shrinkage and membrane blebbing as observed microscopically, DNA fragmentation using agarose gel electrophoresis, and phosphatidylserine (PS) externalization using annexinVimmunostaining. Overall,thesefindingssuggesttheexistenceofapoptosis inA.castellaniipossiblymediatedbyintrinsicapoptoticcascade.Furtherresearchin this field could provide avenues to selectively induce apoptosis in A. castellanii by triggering intrinsic apoptotic cascade. Wiley-VCH Verlag 2017 Article PeerReviewed Baig, Abdul Mannan and Lalani, S.* and Khan, Naveed Ahmed * (2017) Apoptosis in Acanthamoeba castellanii belonging to the T4 genotype. Journal of Basic Microbiology, 9999. pp. 1-6. ISSN 0233 111X |
| spellingShingle | Q Science (General) QR Microbiology Baig, Abdul Mannan Lalani, S.* Khan, Naveed Ahmed * Apoptosis in Acanthamoeba castellanii belonging to the T4 genotype |
| title | Apoptosis in Acanthamoeba castellanii belonging to the T4 genotype |
| title_full | Apoptosis in Acanthamoeba castellanii belonging to the T4 genotype |
| title_fullStr | Apoptosis in Acanthamoeba castellanii belonging to the T4 genotype |
| title_full_unstemmed | Apoptosis in Acanthamoeba castellanii belonging to the T4 genotype |
| title_short | Apoptosis in Acanthamoeba castellanii belonging to the T4 genotype |
| title_sort | apoptosis in acanthamoeba castellanii belonging to the t4 genotype |
| topic | Q Science (General) QR Microbiology |
| url | http://eprints.sunway.edu.my/494/ |