Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine
Dengue virus (DENV) is a major public health threat worldwide. A key element in protection from dengue fever is the neutralising antibody response. Anti-dengue IgG purified from DENV-2 infected human sera showed reactivity against several peptides when evaluated by ELISA and epitope extraction techn...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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PLOS
2016
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| Online Access: | http://eprints.sunway.edu.my/454/ http://eprints.sunway.edu.my/454/1/Ramanathan%202016%20Synthetic%20B.pdf |
| _version_ | 1848801824323141632 |
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| author | Ramanathan, Babu * Poh, Chit Laa * Kirk, Kristin McBride, William John Hannan Aaskov, John Grollo, Lara |
| author_facet | Ramanathan, Babu * Poh, Chit Laa * Kirk, Kristin McBride, William John Hannan Aaskov, John Grollo, Lara |
| author_sort | Ramanathan, Babu * |
| building | SU Institutional Repository |
| collection | Online Access |
| description | Dengue virus (DENV) is a major public health threat worldwide. A key element in protection from dengue fever is the neutralising antibody response. Anti-dengue IgG purified from DENV-2 infected human sera showed reactivity against several peptides when evaluated by ELISA and epitope extraction techniques. A multi-step computational approach predicted six antigenic regions within the E protein of DENV-2 that concur with the 6 epitopes identified by the combined ELISA and epitope extraction approach. The selected peptides
representing B-cell epitopes were attached to a known dengue T-helper epitope and evaluated for their vaccine potency. Immunization of mice revealed two novel synthetic vaccine
constructs that elicited good humoral immune responses and produced cross-reactive neutralising antibodies against DENV-1, 2 and 3. The findings indicate new directions for epitope
mapping and contribute towards the future development of multi-epitope based synthetic peptide vaccine. |
| first_indexed | 2025-11-14T21:13:35Z |
| format | Article |
| id | sunway-454 |
| institution | Sunway University |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T21:13:35Z |
| publishDate | 2016 |
| publisher | PLOS |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | sunway-4542019-07-04T08:04:59Z http://eprints.sunway.edu.my/454/ Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine Ramanathan, Babu * Poh, Chit Laa * Kirk, Kristin McBride, William John Hannan Aaskov, John Grollo, Lara QR Microbiology R Medicine (General) Dengue virus (DENV) is a major public health threat worldwide. A key element in protection from dengue fever is the neutralising antibody response. Anti-dengue IgG purified from DENV-2 infected human sera showed reactivity against several peptides when evaluated by ELISA and epitope extraction techniques. A multi-step computational approach predicted six antigenic regions within the E protein of DENV-2 that concur with the 6 epitopes identified by the combined ELISA and epitope extraction approach. The selected peptides representing B-cell epitopes were attached to a known dengue T-helper epitope and evaluated for their vaccine potency. Immunization of mice revealed two novel synthetic vaccine constructs that elicited good humoral immune responses and produced cross-reactive neutralising antibodies against DENV-1, 2 and 3. The findings indicate new directions for epitope mapping and contribute towards the future development of multi-epitope based synthetic peptide vaccine. PLOS 2016 Article PeerReviewed text en http://eprints.sunway.edu.my/454/1/Ramanathan%202016%20Synthetic%20B.pdf Ramanathan, Babu * and Poh, Chit Laa * and Kirk, Kristin and McBride, William John Hannan and Aaskov, John and Grollo, Lara (2016) Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine. PLOS ONE, 11 (5). e0155900. ISSN 1932-6203 http://dx.doi.org/10.1371/journal.pone.0155900 doi:10.1371/journal.pone.0155900 |
| spellingShingle | QR Microbiology R Medicine (General) Ramanathan, Babu * Poh, Chit Laa * Kirk, Kristin McBride, William John Hannan Aaskov, John Grollo, Lara Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine |
| title | Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine |
| title_full | Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine |
| title_fullStr | Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine |
| title_full_unstemmed | Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine |
| title_short | Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine |
| title_sort | synthetic b-cell epitopes eliciting cross-neutralizing antibodies: strategies for future dengue vaccine |
| topic | QR Microbiology R Medicine (General) |
| url | http://eprints.sunway.edu.my/454/ http://eprints.sunway.edu.my/454/ http://eprints.sunway.edu.my/454/ http://eprints.sunway.edu.my/454/1/Ramanathan%202016%20Synthetic%20B.pdf |