Identification of B-cell epitopes for eliciting neutralizing antibodies against the SARS-CoV-2 spike protein through bioinformatics and monoclonal antibody targeting
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global public health crisis. Effective COVID-19 vaccines developed by Pfizer-BioNTech, Moderna, and Astra Zeneca have made significant impacts in controlling the COVID-19 burden, especially in reducing the transmission of SARS...
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MDPI
2022
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| Online Access: | http://eprints.sunway.edu.my/2172/ http://eprints.sunway.edu.my/2172/1/ijms-23-04341%20%285%29.pdf |
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| author | Lim, Hui Xuan * Masomian, Malihe * Kumar, Asqwin Uthaya MacAry, Paul A. Poh, Chit Laa * |
| author_facet | Lim, Hui Xuan * Masomian, Malihe * Kumar, Asqwin Uthaya MacAry, Paul A. Poh, Chit Laa * |
| author_sort | Lim, Hui Xuan * |
| building | SU Institutional Repository |
| collection | Online Access |
| description | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global public health crisis. Effective COVID-19 vaccines developed by Pfizer-BioNTech, Moderna, and Astra Zeneca have made significant impacts in controlling the COVID-19 burden, especially in reducing the transmission of SARS-CoV-2 and hospitalization incidences. In view of the emergence of new SARS-CoV-2 variants, vaccines developed against the Wuhan strain were less effective against the variants. Neutralizing antibodies produced by B cells are a critical component of adaptive immunity, particularly in neutralizing viruses by blocking virus attachment and entry into cells. Therefore, the identification of protective linear B-cell epitopes can guide epitope-based peptide designs. This study reviews the identification of SARS-CoV-2 B-cell epitopes within the spike, membrane and nucleocapsid proteins that can be incorporated as potent B-cell epitopes into peptide vaccine constructs. The bioinformatic approach offers a new in silico strategy for the mapping and identification of potential B-cell epitopes and, upon in vivo validation, would be useful for the rapid development of effective multi-epitope-based vaccines. Potent B-cell epitopes were identified from the analysis of three-dimensional structures of monoclonal antibodies in a complex with SARS-CoV-2 from literature mining. This review provides significant insights into the elicitation of potential neutralizing antibodies by potent B-cell epitopes, which could advance the development of multiepitope peptide vaccines against SARS-CoV-2. |
| first_indexed | 2025-11-14T21:19:49Z |
| format | Article |
| id | sunway-2172 |
| institution | Sunway University |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T21:19:49Z |
| publishDate | 2022 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | sunway-21722023-04-06T10:03:51Z http://eprints.sunway.edu.my/2172/ Identification of B-cell epitopes for eliciting neutralizing antibodies against the SARS-CoV-2 spike protein through bioinformatics and monoclonal antibody targeting Lim, Hui Xuan * Masomian, Malihe * Kumar, Asqwin Uthaya MacAry, Paul A. Poh, Chit Laa * RA Public aspects of medicine Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global public health crisis. Effective COVID-19 vaccines developed by Pfizer-BioNTech, Moderna, and Astra Zeneca have made significant impacts in controlling the COVID-19 burden, especially in reducing the transmission of SARS-CoV-2 and hospitalization incidences. In view of the emergence of new SARS-CoV-2 variants, vaccines developed against the Wuhan strain were less effective against the variants. Neutralizing antibodies produced by B cells are a critical component of adaptive immunity, particularly in neutralizing viruses by blocking virus attachment and entry into cells. Therefore, the identification of protective linear B-cell epitopes can guide epitope-based peptide designs. This study reviews the identification of SARS-CoV-2 B-cell epitopes within the spike, membrane and nucleocapsid proteins that can be incorporated as potent B-cell epitopes into peptide vaccine constructs. The bioinformatic approach offers a new in silico strategy for the mapping and identification of potential B-cell epitopes and, upon in vivo validation, would be useful for the rapid development of effective multi-epitope-based vaccines. Potent B-cell epitopes were identified from the analysis of three-dimensional structures of monoclonal antibodies in a complex with SARS-CoV-2 from literature mining. This review provides significant insights into the elicitation of potential neutralizing antibodies by potent B-cell epitopes, which could advance the development of multiepitope peptide vaccines against SARS-CoV-2. MDPI 2022-04-14 Article PeerReviewed text en cc_by_4 http://eprints.sunway.edu.my/2172/1/ijms-23-04341%20%285%29.pdf Lim, Hui Xuan * and Masomian, Malihe * and Kumar, Asqwin Uthaya and MacAry, Paul A. and Poh, Chit Laa * (2022) Identification of B-cell epitopes for eliciting neutralizing antibodies against the SARS-CoV-2 spike protein through bioinformatics and monoclonal antibody targeting. International Journal of Molecular Sciences, 23 (8). p. 4341. ISSN 1422-0067 https://doi.org/10.3390/ijms23084341 10.3390/ijms23084341 |
| spellingShingle | RA Public aspects of medicine Lim, Hui Xuan * Masomian, Malihe * Kumar, Asqwin Uthaya MacAry, Paul A. Poh, Chit Laa * Identification of B-cell epitopes for eliciting neutralizing antibodies against the SARS-CoV-2 spike protein through bioinformatics and monoclonal antibody targeting |
| title | Identification of B-cell epitopes for eliciting neutralizing antibodies against the SARS-CoV-2 spike protein through bioinformatics and monoclonal antibody targeting |
| title_full | Identification of B-cell epitopes for eliciting neutralizing antibodies against the SARS-CoV-2 spike protein through bioinformatics and monoclonal antibody targeting |
| title_fullStr | Identification of B-cell epitopes for eliciting neutralizing antibodies against the SARS-CoV-2 spike protein through bioinformatics and monoclonal antibody targeting |
| title_full_unstemmed | Identification of B-cell epitopes for eliciting neutralizing antibodies against the SARS-CoV-2 spike protein through bioinformatics and monoclonal antibody targeting |
| title_short | Identification of B-cell epitopes for eliciting neutralizing antibodies against the SARS-CoV-2 spike protein through bioinformatics and monoclonal antibody targeting |
| title_sort | identification of b-cell epitopes for eliciting neutralizing antibodies against the sars-cov-2 spike protein through bioinformatics and monoclonal antibody targeting |
| topic | RA Public aspects of medicine |
| url | http://eprints.sunway.edu.my/2172/ http://eprints.sunway.edu.my/2172/ http://eprints.sunway.edu.my/2172/ http://eprints.sunway.edu.my/2172/1/ijms-23-04341%20%285%29.pdf |