5-Fluorouracil encapsulated chitosan-cellulose fiber bionanocomposites: Synthesis, characterization and in vitro analysis towards colorectal cancer cells
Cellulose and chitosan with remarkable biocompatibility and sophisticated physiochemical characteristics can be a new dawn to the advanced drug nano-carriers in cancer treatment. This study aims to synthesize layer-by-layer bionanocomposites from chitosan and rice straw cellulose encapsulated 5-Fluo...
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| Format: | Article |
| Language: | English |
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MDPI
2021
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| Online Access: | http://eprints.sunway.edu.my/1789/ http://eprints.sunway.edu.my/1789/1/Teow%20Sin%20Yeang%20Nanomaterials-11-01691.pdf |
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| author | Yusefi, Mostafa Chan, H. Y. Teow, Sin Yeang * Kia, P. Soon, M. L. K. Nor Azwadi Che Sidik, Shameli, Kamyar |
| author_facet | Yusefi, Mostafa Chan, H. Y. Teow, Sin Yeang * Kia, P. Soon, M. L. K. Nor Azwadi Che Sidik, Shameli, Kamyar |
| author_sort | Yusefi, Mostafa |
| building | SU Institutional Repository |
| collection | Online Access |
| description | Cellulose and chitosan with remarkable biocompatibility and sophisticated physiochemical characteristics can be a new dawn to the advanced drug nano-carriers in cancer treatment. This study aims to synthesize layer-by-layer bionanocomposites from chitosan and rice straw cellulose encapsulated 5-Fluorouracil (CS-CF/5FU BNCs) using the ionic gelation method and the sodium tripolyphosphate (TPP) cross-linker. Data from X-ray and Fourier-transform infrared spectroscopy showed successful preparation of CS-CF/5FU BNCs. Based on images of scanning electron microscopy, 48.73 ± 1.52 nm was estimated for an average size of the bionanocomposites as spherical chitosan nanoparticles mostly coated rod-shaped cellulose reinforcement. 5-Fluorouracil indicated an increase in thermal stability after its encapsulation in the bionanocomposites. The drug encapsulation efficiency was found to be 86 ± 2.75%. CS-CF/5FU BNCs triggered higher drug release in a media simulating the colorectal fluid with pH 7.4 (76.82 ± 1.29%) than the gastric fluid with pH 1.2 (42.37 ± 0.43%). In in vitro cytotoxicity assays, cellulose fibers, chitosan nanoparticles and the bionanocomposites indicated biocompatibility towards CCD112 normal cells. Most promisingly, CS-CF/5FU BNCs at 250 µg/mL concentration eliminated 56.42 ± 0.41% of HCT116 cancer cells and only 8.16 ± 2.11% of CCD112 normal cells. Therefore, this study demonstrates that CS-CF/5FU BNCs can be considered as an eco-friendly and innovative nanodrug candidate for potential colorectal cancer treatment. |
| first_indexed | 2025-11-14T21:18:32Z |
| format | Article |
| id | sunway-1789 |
| institution | Sunway University |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T21:18:32Z |
| publishDate | 2021 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | sunway-17892021-09-13T08:20:09Z http://eprints.sunway.edu.my/1789/ 5-Fluorouracil encapsulated chitosan-cellulose fiber bionanocomposites: Synthesis, characterization and in vitro analysis towards colorectal cancer cells Yusefi, Mostafa Chan, H. Y. Teow, Sin Yeang * Kia, P. Soon, M. L. K. Nor Azwadi Che Sidik, Shameli, Kamyar RC0254 Neoplasms. Tumors. Oncology (including Cancer) Cellulose and chitosan with remarkable biocompatibility and sophisticated physiochemical characteristics can be a new dawn to the advanced drug nano-carriers in cancer treatment. This study aims to synthesize layer-by-layer bionanocomposites from chitosan and rice straw cellulose encapsulated 5-Fluorouracil (CS-CF/5FU BNCs) using the ionic gelation method and the sodium tripolyphosphate (TPP) cross-linker. Data from X-ray and Fourier-transform infrared spectroscopy showed successful preparation of CS-CF/5FU BNCs. Based on images of scanning electron microscopy, 48.73 ± 1.52 nm was estimated for an average size of the bionanocomposites as spherical chitosan nanoparticles mostly coated rod-shaped cellulose reinforcement. 5-Fluorouracil indicated an increase in thermal stability after its encapsulation in the bionanocomposites. The drug encapsulation efficiency was found to be 86 ± 2.75%. CS-CF/5FU BNCs triggered higher drug release in a media simulating the colorectal fluid with pH 7.4 (76.82 ± 1.29%) than the gastric fluid with pH 1.2 (42.37 ± 0.43%). In in vitro cytotoxicity assays, cellulose fibers, chitosan nanoparticles and the bionanocomposites indicated biocompatibility towards CCD112 normal cells. Most promisingly, CS-CF/5FU BNCs at 250 µg/mL concentration eliminated 56.42 ± 0.41% of HCT116 cancer cells and only 8.16 ± 2.11% of CCD112 normal cells. Therefore, this study demonstrates that CS-CF/5FU BNCs can be considered as an eco-friendly and innovative nanodrug candidate for potential colorectal cancer treatment. MDPI 2021-06 Article PeerReviewed text en cc_by_nc_4 http://eprints.sunway.edu.my/1789/1/Teow%20Sin%20Yeang%20Nanomaterials-11-01691.pdf Yusefi, Mostafa and Chan, H. Y. and Teow, Sin Yeang * and Kia, P. and Soon, M. L. K. and Nor Azwadi Che Sidik, and Shameli, Kamyar (2021) 5-Fluorouracil encapsulated chitosan-cellulose fiber bionanocomposites: Synthesis, characterization and in vitro analysis towards colorectal cancer cells. Nanomaterials, 11 (7). p. 1691. ISSN 2079-4991 http://doi.org/10.3390/nano11071691 doi:10.3390/nano11071691 |
| spellingShingle | RC0254 Neoplasms. Tumors. Oncology (including Cancer) Yusefi, Mostafa Chan, H. Y. Teow, Sin Yeang * Kia, P. Soon, M. L. K. Nor Azwadi Che Sidik, Shameli, Kamyar 5-Fluorouracil encapsulated chitosan-cellulose fiber bionanocomposites: Synthesis, characterization and in vitro analysis towards colorectal cancer cells |
| title | 5-Fluorouracil encapsulated chitosan-cellulose fiber bionanocomposites: Synthesis, characterization and in vitro analysis towards colorectal cancer cells |
| title_full | 5-Fluorouracil encapsulated chitosan-cellulose fiber bionanocomposites: Synthesis, characterization and in vitro analysis towards colorectal cancer cells |
| title_fullStr | 5-Fluorouracil encapsulated chitosan-cellulose fiber bionanocomposites: Synthesis, characterization and in vitro analysis towards colorectal cancer cells |
| title_full_unstemmed | 5-Fluorouracil encapsulated chitosan-cellulose fiber bionanocomposites: Synthesis, characterization and in vitro analysis towards colorectal cancer cells |
| title_short | 5-Fluorouracil encapsulated chitosan-cellulose fiber bionanocomposites: Synthesis, characterization and in vitro analysis towards colorectal cancer cells |
| title_sort | 5-fluorouracil encapsulated chitosan-cellulose fiber bionanocomposites: synthesis, characterization and in vitro analysis towards colorectal cancer cells |
| topic | RC0254 Neoplasms. Tumors. Oncology (including Cancer) |
| url | http://eprints.sunway.edu.my/1789/ http://eprints.sunway.edu.my/1789/ http://eprints.sunway.edu.my/1789/ http://eprints.sunway.edu.my/1789/1/Teow%20Sin%20Yeang%20Nanomaterials-11-01691.pdf |