Decoding antioxidant and antibacterial potentials of Malaysian green seaweeds: Caulerpa racemosa and Caulerpa lentillifera

Seaweeds are gaining a considerable amount of attention for their antioxidant and antibacterial properties. Caulerpa racemosa and Caulerpa lentillifera, also known as ‘sea grapes’, are green seaweeds commonly found in different parts of the world, but the antioxidant and antibacterial potentials of...

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Main Authors: Yap, W. F., Tay, Vangene, Tan, S. H., Yow, Yoon Yen *, Chew, Jactty *
Format: Article
Language:English
Published: MDPI 2019
Subjects:
Online Access:http://eprints.sunway.edu.my/1720/
http://eprints.sunway.edu.my/1720/1/Yow%20Yoon%20Yen%20Decoding%20antioxidant.pdf
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author Yap, W. F.
Tay, Vangene
Tan, S. H.
Yow, Yoon Yen *
Chew, Jactty *
author_facet Yap, W. F.
Tay, Vangene
Tan, S. H.
Yow, Yoon Yen *
Chew, Jactty *
author_sort Yap, W. F.
building SU Institutional Repository
collection Online Access
description Seaweeds are gaining a considerable amount of attention for their antioxidant and antibacterial properties. Caulerpa racemosa and Caulerpa lentillifera, also known as ‘sea grapes’, are green seaweeds commonly found in different parts of the world, but the antioxidant and antibacterial potentials of Malaysian C. racemosa and C. lentillifera have not been thoroughly explored. In this study, crude extracts of the seaweeds were prepared using chloroform, methanol, and water. Total phenolic content (TPC) and total flavonoid content (TFC) were measured, followed by in vitro antioxidant activity determination using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Antibacterial activities of these extracts were tested against Methicillin-resistant Staphylococcus aureus (MRSA) and neuropathogenic Escherichia coli K1. Liquid chromatography–mass spectrometry (LCMS) analysis was then used to determine the possible compounds present in the extract with the most potent antioxidant and antibacterial activity. Results showed that C. racemosa chloroform extract had the highest TPC (13.41 ± 0.86 mg GAE/g), antioxidant effect (EC50 at 0.65 ± 0.03 mg/mL), and the strongest antibacterial effect (97.7 ± 0.30%) against MRSA. LCMS analysis proposed that the chloroform extracts of C. racemosa are mainly polyunsaturated and monounsaturated fatty acids, terpenes, and alkaloids. In conclusion, C. racemosa can be a great source of novel antioxidant and antibacterial agents, but isolation and purification of the bioactive compounds are needed to study their mechanism of action.
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spelling sunway-17202021-04-15T01:55:57Z http://eprints.sunway.edu.my/1720/ Decoding antioxidant and antibacterial potentials of Malaysian green seaweeds: Caulerpa racemosa and Caulerpa lentillifera Yap, W. F. Tay, Vangene Tan, S. H. Yow, Yoon Yen * Chew, Jactty * QH301 Biology Seaweeds are gaining a considerable amount of attention for their antioxidant and antibacterial properties. Caulerpa racemosa and Caulerpa lentillifera, also known as ‘sea grapes’, are green seaweeds commonly found in different parts of the world, but the antioxidant and antibacterial potentials of Malaysian C. racemosa and C. lentillifera have not been thoroughly explored. In this study, crude extracts of the seaweeds were prepared using chloroform, methanol, and water. Total phenolic content (TPC) and total flavonoid content (TFC) were measured, followed by in vitro antioxidant activity determination using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Antibacterial activities of these extracts were tested against Methicillin-resistant Staphylococcus aureus (MRSA) and neuropathogenic Escherichia coli K1. Liquid chromatography–mass spectrometry (LCMS) analysis was then used to determine the possible compounds present in the extract with the most potent antioxidant and antibacterial activity. Results showed that C. racemosa chloroform extract had the highest TPC (13.41 ± 0.86 mg GAE/g), antioxidant effect (EC50 at 0.65 ± 0.03 mg/mL), and the strongest antibacterial effect (97.7 ± 0.30%) against MRSA. LCMS analysis proposed that the chloroform extracts of C. racemosa are mainly polyunsaturated and monounsaturated fatty acids, terpenes, and alkaloids. In conclusion, C. racemosa can be a great source of novel antioxidant and antibacterial agents, but isolation and purification of the bioactive compounds are needed to study their mechanism of action. MDPI 2019 Article PeerReviewed text en cc_by_nc_4 http://eprints.sunway.edu.my/1720/1/Yow%20Yoon%20Yen%20Decoding%20antioxidant.pdf Yap, W. F. and Tay, Vangene and Tan, S. H. and Yow, Yoon Yen * and Chew, Jactty * (2019) Decoding antioxidant and antibacterial potentials of Malaysian green seaweeds: Caulerpa racemosa and Caulerpa lentillifera. Antibiotics, 8 (3). p. 152. ISSN 2079-6382 http://doi.org/10.3390/antibiotics8030152 doi:10.3390/antibiotics8030152
spellingShingle QH301 Biology
Yap, W. F.
Tay, Vangene
Tan, S. H.
Yow, Yoon Yen *
Chew, Jactty *
Decoding antioxidant and antibacterial potentials of Malaysian green seaweeds: Caulerpa racemosa and Caulerpa lentillifera
title Decoding antioxidant and antibacterial potentials of Malaysian green seaweeds: Caulerpa racemosa and Caulerpa lentillifera
title_full Decoding antioxidant and antibacterial potentials of Malaysian green seaweeds: Caulerpa racemosa and Caulerpa lentillifera
title_fullStr Decoding antioxidant and antibacterial potentials of Malaysian green seaweeds: Caulerpa racemosa and Caulerpa lentillifera
title_full_unstemmed Decoding antioxidant and antibacterial potentials of Malaysian green seaweeds: Caulerpa racemosa and Caulerpa lentillifera
title_short Decoding antioxidant and antibacterial potentials of Malaysian green seaweeds: Caulerpa racemosa and Caulerpa lentillifera
title_sort decoding antioxidant and antibacterial potentials of malaysian green seaweeds: caulerpa racemosa and caulerpa lentillifera
topic QH301 Biology
url http://eprints.sunway.edu.my/1720/
http://eprints.sunway.edu.my/1720/
http://eprints.sunway.edu.my/1720/
http://eprints.sunway.edu.my/1720/1/Yow%20Yoon%20Yen%20Decoding%20antioxidant.pdf