Gracilaria changii (Rhodophyta) alleviates bisphenol A-induced adverse reproductive abnormalities in mice

Objective: To evaluate the potential of Gracilaria changii extract in ameliorating the potential adverse effects of bisphenol A. Methods: The antioxidant capacity of Gracilaria changii extracted using different solvents (methanol, ethanol, and aqueous) was studied. The mice were administered by ora...

Full description

Bibliographic Details
Main Authors: Kwong, P. J., Ng, C. L., Tan, G. C., Yow, Yoon Yen *, Gupta, M. K.
Format: Article
Language:English
Published: Hainan Medical University 2021
Subjects:
Online Access:http://eprints.sunway.edu.my/1693/
http://eprints.sunway.edu.my/1693/1/Yow%20yoon%20yen%20Gracilaria%20changii.pdf
_version_ 1848802113574928384
author Kwong, P. J.
Ng, C. L.
Tan, G. C.
Yow, Yoon Yen *
Gupta, M. K.
author_facet Kwong, P. J.
Ng, C. L.
Tan, G. C.
Yow, Yoon Yen *
Gupta, M. K.
author_sort Kwong, P. J.
building SU Institutional Repository
collection Online Access
description Objective: To evaluate the potential of Gracilaria changii extract in ameliorating the potential adverse effects of bisphenol A. Methods: The antioxidant capacity of Gracilaria changii extracted using different solvents (methanol, ethanol, and aqueous) was studied. The mice were administered by oral gavage with bisphenol A (60 mg/kg body weight) for 6 weeks with or without Gracilaria changii aqueous extract. Thereafter, the mice were either euthanized for histology and immunohistochemistry studies or mated to evaluate the pregnancy rate. Results: Gracilaria changii aqueous extract showed the highest antioxidant properties compared with extract using methanol and ethanol. The aqueous extract of Gracilaria changii improved the uterus index and uterine lipid peroxidation after bisphenol A exposure, although the uterine expressions of estrogen receptors and complement C3 were not improved. Histological evaluation of the uterus during the estrus stage has revealed that the extract could mitigate bisphenol A-induced adverse effects in the uterus as there was a lower percentage of mice showing abnormalities like decreased eosin staining in the myometrium, and decrease in the number of eosinophil and endometrial glands in the endometrium. Besides, Gracilaria changii aqueous extract improved the pregnancy rate of mice administered with bisphenol A. Conclusions: Gracilaria changii extract protects against bisphenol A-induced female reproductive abnormalities in mice which may be mediated via modulation of eosinophil migration, endometrial gland formation, and protein expressions associated with prostaglandins in the myometrium.
first_indexed 2025-11-14T21:18:11Z
format Article
id sunway-1693
institution Sunway University
institution_category Local University
language English
last_indexed 2025-11-14T21:18:11Z
publishDate 2021
publisher Hainan Medical University
recordtype eprints
repository_type Digital Repository
spelling sunway-16932021-03-22T08:01:11Z http://eprints.sunway.edu.my/1693/ Gracilaria changii (Rhodophyta) alleviates bisphenol A-induced adverse reproductive abnormalities in mice Kwong, P. J. Ng, C. L. Tan, G. C. Yow, Yoon Yen * Gupta, M. K. QH301 Biology Objective: To evaluate the potential of Gracilaria changii extract in ameliorating the potential adverse effects of bisphenol A. Methods: The antioxidant capacity of Gracilaria changii extracted using different solvents (methanol, ethanol, and aqueous) was studied. The mice were administered by oral gavage with bisphenol A (60 mg/kg body weight) for 6 weeks with or without Gracilaria changii aqueous extract. Thereafter, the mice were either euthanized for histology and immunohistochemistry studies or mated to evaluate the pregnancy rate. Results: Gracilaria changii aqueous extract showed the highest antioxidant properties compared with extract using methanol and ethanol. The aqueous extract of Gracilaria changii improved the uterus index and uterine lipid peroxidation after bisphenol A exposure, although the uterine expressions of estrogen receptors and complement C3 were not improved. Histological evaluation of the uterus during the estrus stage has revealed that the extract could mitigate bisphenol A-induced adverse effects in the uterus as there was a lower percentage of mice showing abnormalities like decreased eosin staining in the myometrium, and decrease in the number of eosinophil and endometrial glands in the endometrium. Besides, Gracilaria changii aqueous extract improved the pregnancy rate of mice administered with bisphenol A. Conclusions: Gracilaria changii extract protects against bisphenol A-induced female reproductive abnormalities in mice which may be mediated via modulation of eosinophil migration, endometrial gland formation, and protein expressions associated with prostaglandins in the myometrium. Hainan Medical University 2021-01-05 Article PeerReviewed text en cc_by_nc_nd_4 http://eprints.sunway.edu.my/1693/1/Yow%20yoon%20yen%20Gracilaria%20changii.pdf Kwong, P. J. and Ng, C. L. and Tan, G. C. and Yow, Yoon Yen * and Gupta, M. K. (2021) Gracilaria changii (Rhodophyta) alleviates bisphenol A-induced adverse reproductive abnormalities in mice. Asian Pacific Journal of Tropical Medicine, 14 (1). pp. 34-43. ISSN 1995-7645 http://doi.org/10.4103/1995-7645.304299 doi:10.4103/1995-7645.304299
spellingShingle QH301 Biology
Kwong, P. J.
Ng, C. L.
Tan, G. C.
Yow, Yoon Yen *
Gupta, M. K.
Gracilaria changii (Rhodophyta) alleviates bisphenol A-induced adverse reproductive abnormalities in mice
title Gracilaria changii (Rhodophyta) alleviates bisphenol A-induced adverse reproductive abnormalities in mice
title_full Gracilaria changii (Rhodophyta) alleviates bisphenol A-induced adverse reproductive abnormalities in mice
title_fullStr Gracilaria changii (Rhodophyta) alleviates bisphenol A-induced adverse reproductive abnormalities in mice
title_full_unstemmed Gracilaria changii (Rhodophyta) alleviates bisphenol A-induced adverse reproductive abnormalities in mice
title_short Gracilaria changii (Rhodophyta) alleviates bisphenol A-induced adverse reproductive abnormalities in mice
title_sort gracilaria changii (rhodophyta) alleviates bisphenol a-induced adverse reproductive abnormalities in mice
topic QH301 Biology
url http://eprints.sunway.edu.my/1693/
http://eprints.sunway.edu.my/1693/
http://eprints.sunway.edu.my/1693/
http://eprints.sunway.edu.my/1693/1/Yow%20yoon%20yen%20Gracilaria%20changii.pdf