The potential anticancer activity of 5-fluorouracil loaded in cellulose fibers isolated from rice straw
Introduction: Green-based materials have been increasingly studied to circumvent off-target cytotoxicity and other side-effects from conventional chemotherapy. Materials and Methods: Here, cellulose fibers (CF) were isolated from rice straw (RS) waste by using an eco-friendly alkali treatment. The...
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Dove Medical Press
2020
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| Online Access: | http://eprints.sunway.edu.my/1563/ http://eprints.sunway.edu.my/1563/1/Teow%20Sin%20Yeang%20The%20Potential%20Anticancer%20Activity%20of%205-Fluorouracil%20Loaded%20in%20Cellulose%20Fibers%20Isolated%20from%20Rice%20Straw.pdf |
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| author | Yusefi, Mostafa Shameli, Kamyar Jahangirian, Hossein Teow, Sin Yeang * Umakoshi, Hiroshi Saleh, Bahram Rafiee-Moghaddam, Roshanak Webster, Thomas |
| author_facet | Yusefi, Mostafa Shameli, Kamyar Jahangirian, Hossein Teow, Sin Yeang * Umakoshi, Hiroshi Saleh, Bahram Rafiee-Moghaddam, Roshanak Webster, Thomas |
| author_sort | Yusefi, Mostafa |
| building | SU Institutional Repository |
| collection | Online Access |
| description | Introduction: Green-based materials have been increasingly studied to circumvent off-target cytotoxicity and other side-effects from conventional chemotherapy.
Materials and Methods: Here, cellulose fibers (CF) were isolated from rice straw (RS) waste by using an eco-friendly alkali treatment. The CF network served as an anticancer drug carrier for 5-fluorouracil (5-FU). The physicochemical and thermal properties of CF, pure 5-FU drug, and the 5-FU-loaded CF (CF/5-FU) samples were evaluated. The samples were assessed for in vitro cytotoxicity assays using human colorectal cancer (HCT116) and normal (CCD112) cell lines, along with human nasopharyngeal cancer (HONE-1) and normal (NP 460) cell lines after 72-hours of treatment.
Results: XRD and FTIR revealed the successful alkali treatment of RS to isolate CF with high purity and crystallinity. Compared to RS, the alkali-treated CF showed an almost fourfold increase in surface area and zeta potential of up to − 33.61 mV. SEM images illustrated the CF network with a rod-shaped structure and comprised of ordered aggregated cellulose. TGA results proved that the thermal stability of 5-FU increased within the drug carrier. Based on UV-spectroscopy measurements for 5-FU loading into CF, drug loading encapsulation efficiency was estimated to be 83 ± 0.8%. The release media at pH 7.4 and pH 1.2 showed a maximum drug release of 79% and 46%, respectively, over 24 hours. In cytotoxicity assays, CF showed almost no damage, while pure 5-FU killed most of the both normal and cancer cells. Impressively, the drug-loaded sample of CF/5-FU at a 250 μg/mL concentration demonstrated a 58% inhibition against colorectal cancer cells, but only a 23% inhibition against normal colorectal cells. Further, a 62.50 μg/mL concentration of CF/5FU eliminated 71% and 39% of nasopharyngeal carcinoma and normal nasopharyngeal cells, respectively.
Discussion: This study, therefore, showed the strong potential anticancer activity of the novel CF/5-FU formulations, warranting their further investigation. |
| first_indexed | 2025-11-14T21:17:46Z |
| format | Article |
| id | sunway-1563 |
| institution | Sunway University |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T21:17:46Z |
| publishDate | 2020 |
| publisher | Dove Medical Press |
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| spelling | sunway-15632021-01-27T02:34:59Z http://eprints.sunway.edu.my/1563/ The potential anticancer activity of 5-fluorouracil loaded in cellulose fibers isolated from rice straw Yusefi, Mostafa Shameli, Kamyar Jahangirian, Hossein Teow, Sin Yeang * Umakoshi, Hiroshi Saleh, Bahram Rafiee-Moghaddam, Roshanak Webster, Thomas QH301 Biology RC0254 Neoplasms. Tumors. Oncology (including Cancer) Introduction: Green-based materials have been increasingly studied to circumvent off-target cytotoxicity and other side-effects from conventional chemotherapy. Materials and Methods: Here, cellulose fibers (CF) were isolated from rice straw (RS) waste by using an eco-friendly alkali treatment. The CF network served as an anticancer drug carrier for 5-fluorouracil (5-FU). The physicochemical and thermal properties of CF, pure 5-FU drug, and the 5-FU-loaded CF (CF/5-FU) samples were evaluated. The samples were assessed for in vitro cytotoxicity assays using human colorectal cancer (HCT116) and normal (CCD112) cell lines, along with human nasopharyngeal cancer (HONE-1) and normal (NP 460) cell lines after 72-hours of treatment. Results: XRD and FTIR revealed the successful alkali treatment of RS to isolate CF with high purity and crystallinity. Compared to RS, the alkali-treated CF showed an almost fourfold increase in surface area and zeta potential of up to − 33.61 mV. SEM images illustrated the CF network with a rod-shaped structure and comprised of ordered aggregated cellulose. TGA results proved that the thermal stability of 5-FU increased within the drug carrier. Based on UV-spectroscopy measurements for 5-FU loading into CF, drug loading encapsulation efficiency was estimated to be 83 ± 0.8%. The release media at pH 7.4 and pH 1.2 showed a maximum drug release of 79% and 46%, respectively, over 24 hours. In cytotoxicity assays, CF showed almost no damage, while pure 5-FU killed most of the both normal and cancer cells. Impressively, the drug-loaded sample of CF/5-FU at a 250 μg/mL concentration demonstrated a 58% inhibition against colorectal cancer cells, but only a 23% inhibition against normal colorectal cells. Further, a 62.50 μg/mL concentration of CF/5FU eliminated 71% and 39% of nasopharyngeal carcinoma and normal nasopharyngeal cells, respectively. Discussion: This study, therefore, showed the strong potential anticancer activity of the novel CF/5-FU formulations, warranting their further investigation. Dove Medical Press 2020-07-29 Article PeerReviewed text en cc_by_nc_4 http://eprints.sunway.edu.my/1563/1/Teow%20Sin%20Yeang%20The%20Potential%20Anticancer%20Activity%20of%205-Fluorouracil%20Loaded%20in%20Cellulose%20Fibers%20Isolated%20from%20Rice%20Straw.pdf Yusefi, Mostafa and Shameli, Kamyar and Jahangirian, Hossein and Teow, Sin Yeang * and Umakoshi, Hiroshi and Saleh, Bahram and Rafiee-Moghaddam, Roshanak and Webster, Thomas (2020) The potential anticancer activity of 5-fluorouracil loaded in cellulose fibers isolated from rice straw. International Journal of Nanomedicine, 15. pp. 5417-5432. ISSN 1178-2013 http://doi.org/10.2147/IJN.S250047 doi:10.2147/IJN.S250047 |
| spellingShingle | QH301 Biology RC0254 Neoplasms. Tumors. Oncology (including Cancer) Yusefi, Mostafa Shameli, Kamyar Jahangirian, Hossein Teow, Sin Yeang * Umakoshi, Hiroshi Saleh, Bahram Rafiee-Moghaddam, Roshanak Webster, Thomas The potential anticancer activity of 5-fluorouracil loaded in cellulose fibers isolated from rice straw |
| title | The potential anticancer activity of 5-fluorouracil loaded in cellulose fibers isolated from rice straw |
| title_full | The potential anticancer activity of 5-fluorouracil loaded in cellulose fibers isolated from rice straw |
| title_fullStr | The potential anticancer activity of 5-fluorouracil loaded in cellulose fibers isolated from rice straw |
| title_full_unstemmed | The potential anticancer activity of 5-fluorouracil loaded in cellulose fibers isolated from rice straw |
| title_short | The potential anticancer activity of 5-fluorouracil loaded in cellulose fibers isolated from rice straw |
| title_sort | potential anticancer activity of 5-fluorouracil loaded in cellulose fibers isolated from rice straw |
| topic | QH301 Biology RC0254 Neoplasms. Tumors. Oncology (including Cancer) |
| url | http://eprints.sunway.edu.my/1563/ http://eprints.sunway.edu.my/1563/ http://eprints.sunway.edu.my/1563/ http://eprints.sunway.edu.my/1563/1/Teow%20Sin%20Yeang%20The%20Potential%20Anticancer%20Activity%20of%205-Fluorouracil%20Loaded%20in%20Cellulose%20Fibers%20Isolated%20from%20Rice%20Straw.pdf |