Development of multi-epitope peptide-based vaccines against SARS-CoV-2
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a pandemic involving so far more than 15 million infections and 630,211 deaths. Effective vaccines are urgently needed to prevent SARS-CoV-2 infections. No vaccines have yet been approved for licensure by regulatory agencies. Ev...
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| Format: | Article |
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Elsevier
2020
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| Online Access: | http://eprints.sunway.edu.my/1532/ http://eprints.sunway.edu.my/1532/1/lim%20xuan%20lim%20develoment%20of%20multi%20epitope.pdf |
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| author | Lim, Hui Xuan * Lim, Jian Hua * Jazayeri, S. D. * Poppema, Sibrandes * Poh, Chit Laa * |
| author_facet | Lim, Hui Xuan * Lim, Jian Hua * Jazayeri, S. D. * Poppema, Sibrandes * Poh, Chit Laa * |
| author_sort | Lim, Hui Xuan * |
| building | SU Institutional Repository |
| collection | Online Access |
| description | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a pandemic involving so far more than 15 million infections and 630,211 deaths. Effective vaccines are urgently needed to prevent SARS-CoV-2 infections. No vaccines have yet been approved for licensure by regulatory agencies. Even though host immune responses to SARS-CoV-2 infections are beginning to be unravelled, effective clearance of virus will depend on both humoral and cellular immunity. Additionally, the presence of Spike (S)-glycoprotein reactive CD4+ T-cells in the majority of convalescent patients is consistent with its significant role in stimulating B and CD8+ T-cells. The search for immunodominant epitopes relies on experimental evaluation of peptides representing the epitopes from overlapping peptide libraries which can be costly and labor-intensive. Recent advancements in B- and T-cell epitope predictions by bioinformatic analysis have led to epitope identifications. Assessing which peptide epitope can induce potent neutralizing antibodies and robust T-cell responses is a prerequisite for the selection of effective epitopes to be incorporated in peptide-based vaccines. This review discusses the roles of B- and T-cells in SARS-CoV-2 infections and experimental validations for the selection of B-, CD4+ and CD8+ T-cell epitopes which could lead to the construction of a multi-epitope peptide vaccine. Peptide-based vaccines are known for their low immunogenicity which could be overcome by incorporating immunostimulatory adjuvants and nanoparticles such as Poly Lactic-co-Glycolic Acid (PLGA) or chitosan. |
| first_indexed | 2025-11-14T21:17:39Z |
| format | Article |
| id | sunway-1532 |
| institution | Sunway University |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T21:17:39Z |
| publishDate | 2020 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | sunway-15322021-05-28T06:20:56Z http://eprints.sunway.edu.my/1532/ Development of multi-epitope peptide-based vaccines against SARS-CoV-2 Lim, Hui Xuan * Lim, Jian Hua * Jazayeri, S. D. * Poppema, Sibrandes * Poh, Chit Laa * QR Microbiology QR355 Virology Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a pandemic involving so far more than 15 million infections and 630,211 deaths. Effective vaccines are urgently needed to prevent SARS-CoV-2 infections. No vaccines have yet been approved for licensure by regulatory agencies. Even though host immune responses to SARS-CoV-2 infections are beginning to be unravelled, effective clearance of virus will depend on both humoral and cellular immunity. Additionally, the presence of Spike (S)-glycoprotein reactive CD4+ T-cells in the majority of convalescent patients is consistent with its significant role in stimulating B and CD8+ T-cells. The search for immunodominant epitopes relies on experimental evaluation of peptides representing the epitopes from overlapping peptide libraries which can be costly and labor-intensive. Recent advancements in B- and T-cell epitope predictions by bioinformatic analysis have led to epitope identifications. Assessing which peptide epitope can induce potent neutralizing antibodies and robust T-cell responses is a prerequisite for the selection of effective epitopes to be incorporated in peptide-based vaccines. This review discusses the roles of B- and T-cells in SARS-CoV-2 infections and experimental validations for the selection of B-, CD4+ and CD8+ T-cell epitopes which could lead to the construction of a multi-epitope peptide vaccine. Peptide-based vaccines are known for their low immunogenicity which could be overcome by incorporating immunostimulatory adjuvants and nanoparticles such as Poly Lactic-co-Glycolic Acid (PLGA) or chitosan. Elsevier 2020-10-01 Article PeerReviewed text en cc_by_nc_4 http://eprints.sunway.edu.my/1532/1/lim%20xuan%20lim%20develoment%20of%20multi%20epitope.pdf Lim, Hui Xuan * and Lim, Jian Hua * and Jazayeri, S. D. * and Poppema, Sibrandes * and Poh, Chit Laa * (2020) Development of multi-epitope peptide-based vaccines against SARS-CoV-2. Biomedical Journal, 44 (1). pp. 18-30. ISSN 2319-4170 http://doi.org/10.1016/j.bj.2020.09.005 doi:10.1016/j.bj.2020.09.005 |
| spellingShingle | QR Microbiology QR355 Virology Lim, Hui Xuan * Lim, Jian Hua * Jazayeri, S. D. * Poppema, Sibrandes * Poh, Chit Laa * Development of multi-epitope peptide-based vaccines against SARS-CoV-2 |
| title | Development of multi-epitope peptide-based vaccines against SARS-CoV-2 |
| title_full | Development of multi-epitope peptide-based vaccines against SARS-CoV-2 |
| title_fullStr | Development of multi-epitope peptide-based vaccines against SARS-CoV-2 |
| title_full_unstemmed | Development of multi-epitope peptide-based vaccines against SARS-CoV-2 |
| title_short | Development of multi-epitope peptide-based vaccines against SARS-CoV-2 |
| title_sort | development of multi-epitope peptide-based vaccines against sars-cov-2 |
| topic | QR Microbiology QR355 Virology |
| url | http://eprints.sunway.edu.my/1532/ http://eprints.sunway.edu.my/1532/ http://eprints.sunway.edu.my/1532/ http://eprints.sunway.edu.my/1532/1/lim%20xuan%20lim%20develoment%20of%20multi%20epitope.pdf |